Imaging single plant cells

Holscher, D., Shroff, R., Knop, K., Gottschaldt, M., Crecelius, A., Schneider, B., Heckel, D. G., Schubert, U. S., Svatos, A. (2009) Matrix-free UV-laser des-orption/ionization (LDI) mass spectromet-ric imaging on the single-cell level distribution of secondary metabolites of Arabidopsis thaliana and Hypericum species. Plant/, 60, 907-918. 

Fig. 13.17. SEM image of the ablation crater produced by a single laser pulse on the ad-axial leaf surface of a Zebra plant Aphelandra squarrosa). Scale bar indicates 200 pm. Multiple laser pulses penetrated deeper. The wrinkles on the cuticle were caused by the collapse of the underlying cells due to water loss in the vacuum enviromnent during SEM imaging. Adapted from Ref. [53] with permission. The American Chemical Society, 2008.

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