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Cell Imaging of mRNA Using PNA FIT Probes

Life Cell Imaging of mRNA Using PNA FIT Probes [Pg.351]

Andrea Knoll, Susann Kummer, Felix Hovelmann, Andreas Herrmann, and Oliver Seitz [Pg.351]

Methods to study the biosynthesis of RNA in living cells, their intracellular transport, subcellular localization, and degradation are of great interest for understanding cellular networks and their malfunction [1-3] during diseases. Here, we describe the live cell RNA imaging with peptide nucleic acid-based FIT forced intercalation probes that enabled a simultaneous localization of two viral messenger ribonucleic acid (mRNA) molecules. [Pg.351]



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Cells imaging

Image Using

Image probing

Imaging probes

Imaging using

MRNA

PNA

PNA probe

Useful image

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