Calcium imaging single-cell

Most of the fluorescent calcium indicators and their cell-permeant acetoxymethyl (AM) esters are variations of the nonfluorescent calcium chelator BAPTA and have been proposed by Tsien/134-1365 Among them Fura-2 and Indo-1 (Figure 5.22) are particularly used formeasuring Ca2+in single cells by imaging or flow cytometry/65... 

Total K, Na, and Ca concentrations calculated from SIMS ion microscopy images of single cells shown in control (Fig. 6.5) and cisplatin-treated (Fig. 6.6) renal epithelial LLC-PKi cells. Concentrations in control cells are listed as the mean SD for all cells shown in Fig. 6.5. For cisplatin-treated cells, concentrations are listed in individual cells identified by the cell numbers in Fig. 6.6. Total Ca concentrations reflect the level of calcium stores in designated subcellular compartments... 

Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry Cisplatin Affects Calcium... 

Using fluorescent indicators with single-cell video imaging, the [Ca], responses of individual parietal cells are typically found to be biphasic, consisting of an initial peak elevation that rapidly declines to a sustained steady-state elevation. This pattern of [Ca]i response is seen in many cell types and is interpreted to result from an initial release of calcium from intracellular storage sites followed by a sustained influx of calcium from the extracellular fluid via a receptor-operated Ca channel. Similar mechanisms appear to be involved in the agonist-induced elevation of [Ca], of parietal cells. Accordingly,... 

Bolton TB, Gordienko DV 1998 Confocal imaging of calcium release events in single smooth muscle cells. Acta Physiol Scand 164 567—575... 

We believe that single molecule imaging can contribute to a more thorough understanding of the role of pectin in the cell wall. Our future efforts will be focused on pectin gels formed in the presence of calcium. Eventually, it should be possible to visualize side chains on pectin and determine how rhamnose residues in the rhamnogalacturonan backbone of tobacco pectin disrupt the formation of helical regions. 

Chandra, S. (2008) Subcellular imaging of RNA distribution and DNA replication in single mammalian cells with SIMS the localization of heat shock induced RNA in relation to the distribution of intranuclear bound calcium. J Microsc, 232, 27-35. 

Single compartment models are desirable in this work, because any interaction between the mathematical models and the corresponding experimental systems should be based on the same known quantities. It would not be reasonable to include variables in the models that either cannot be determined from separate equations or cannot be provided as an input from the available experimental data. To check and validate models, we are using microscopy and imaging techniques to capture dynamic calcium events taking place within a cell as a whole, as opposed to calcium diffusion across a cell or intracellular calcium waves, for example (Patterson et al., 2007). 

Demuro, A. Parker, I. Optical single-channel recording imaging Ca flux through individual A-type voltage-gated channels expressed in Xenopus oocytes. Cell Calcium 2003, 34, 499-509. 

Churchill, G. C. Louis, C. F. Imaging of intracellular calcium stores in single permeabilized lens cells. Am. J. Physiol. 1999, 276, C426-C434. 

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