Intra-cell mapping of drug delivery using Raman imaging

Intra-cell mapping of drug delivery using Raman imaging 

as weU as common chemotherapy of cancers, critically depends on the (overwhelmingly) selective absorption of the administered drug by the cancer cell healthy cells should, as far as possible, not be destroyed. Therefore, the need for cost-effective and efficient approaches to evaluate the effectiveness of selective accumulation in the cancer cell is unquestionable. 

we describe one representative example, namely the application of direct Raman imaging to the visualization of an anticancer drug, paclitaxel, within living tumour cells (see Ling et al. (2002)). Paclitaxel is an important antimitotic agent whose mechanisms of interaction with cells are reasonably well understood thus, it can be viewed as a suitable candidate for validation of the capabilities of Raman imaging. The human breast tumour cell line MDA-435 was used in these studies. 

Note that in the methodology described here the laser serves as an analytical tool (in the form of Raman spectroscopy) to follow the chemical reaction process induced by the drug this is in contrast to PDT, 

The Raman peak at 617 cm is due to deformation of benzene rings in the stmcture, the strong 

---

INTRA-CELL MAPPING OF DRUG DELIVERY USING RAMAN IMAGING... 

---