Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Cyclodextrins capillary electrophoresis

Conradi, S. Vogt, C. Rohde, E. Separation of Enatiomeric Barbiturates by Capillary Electrophoresis Using a Cyclodextrin-Containing Run Buffer, /. Chem. Educ. 1997, 74, 1122-1125. [Pg.614]

A stereoselective determination of enantiomers of 5, its A -oxide and N-desmethyl metabolites in human urine was developed by capillary electrophoresis using laser-induced fluorescence detection and sulfonated /1-cyclodextrin in the running buffer (01JC(B)169). [Pg.266]

Enantioresolution in capillary electrophoresis (CE) is typically achieved with the help of chiral additives dissolved in the background electrolyte. A number of low as well as high molecular weight compounds such as proteins, antibiotics, crown ethers, and cyclodextrins have already been tested and optimized. Since the mechanism of retention and resolution remains ambiguous, the selection of an additive best suited for the specific separation relies on the one-at-a-time testing of each individual compound, a tedious process at best. Obviously, the use of a mixed library of chiral additives combined with an efficient deconvolution strategy has the potential to accelerate this selection. [Pg.62]

Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. Conventionally, instrumental chiral separations have been achieved by gas chromatography and by high performance liquid chromatography.127 In recent years, there has been considerable activity in the separation and characterization of racemic pharmaceuticals by high performance capillary electrophoresis, with particular interest paid to using this technique in modem pharmaceutical analytical laboratories.128 130 The most frequently used chiral selectors in CE are cyclodextrins, crown ethers, chiral surfactants, bile acids, and protein-filled... [Pg.405]

Miniaturized columns have provided a decisive advantage in speed. Uracil, phenol, and benzyl alcohol were separated in 20 seconds by CEC in an 18 mm column with a propyl reversed phase.29 A19 cm electrophoretic channel was etched into a glass wafer, filled with a y-cyclodextrin buffer, and used to resolve chiral amino acids from a meteorite in 4 minutes.30 A 6 cm channel equipped with a syringe pump to automate sample derivatization was used to separate amino acids modified with fluorescein isothiocyanate.31 Nanovials have been used to perform tryptic digests on the 15 nL scale for subsequent separation on capillary Electrophoresis.32 A microcolumn has also been used to generate fractions representing time-points of digestion from a 40 pL sample.33 A disposable nanoelectrospray emitter has been... [Pg.429]

Kang, J. and Ou, Q., Chiral separation of racemic mexilitine hydrochloride using cyclodextrins as chiral additive by capillary electrophoresis,. Chromatogr. A, 795, 394, 1998. [Pg.439]

Lin et al. [95] used capillary electrophoresis with dual cyclodextrin systems for the enantiomer separation of miconazole. A cyclodextrin-modified micellar capillary electrophoretic method was developed using mixture of /i-cyclodextrins and mono-3-0-phenylcarbamoyl-/j-cyclodextrin as chiral additives for the chiral separation of miconazole with the dual cyclodextrins systems. The enantiomers were resolved using a running buffer of 50 mmol/L borate pH 9.5 containing 15 mmol/L jS-cyclodextrin and 15 mmol/L mono-3-<9-phcnylcarbamoyl-/j-cyclodextrin containing 50 mmol/L sodium dodecyl sulfate and 1 mol/L urea. A study of the respective influence of the /i-cyclodcxtrin and the mono-3-(9-phenylcarbamoyl-/i-cyclodextrin concentration was performed to determine the optical conditions with respect to the resolution. Good repeatability of the method was obtained. [Pg.55]

Organolead and organoselenium compounds were separated satisfactorily by high-performance capillary electrophoresis, using /1-cyclodextrin-modified micellar electroki-netic chromatography with on-column UVV detector set at 210 nm130. [Pg.442]

Malekinejad H, Schoevers EJ, Daemen IJ, Zijlstra C, Colenbrander B, Fink-Gremmels F, Roelen BAJ (2007) Exposure of oocytes to the Fusarium toxins zearalenone and deoxynivalenol causes aneuploidy and abnormal embryo development in pigs. Biol Reprod 77 840-847 Maragos CM, Appell MD (2007) Capillary electrophoresis of the mycotoxin zearalenone using cyclodextrin-enhanced fluorescence. J Chromatogr A 1143 252-257... [Pg.433]

Anions and uncharged analytes tend to spend more time in the buffered solution and as a result their movement relates to this. While these are useful generalizations, various factors contribute to the migration order of the analytes. These include the anionic or cationic nature of the surfactant, the influence of electroendosmosis, the properties of the buffer, the contributions of electrostatic versus hydrophobic interactions and the electrophoretic mobility of the native analyte. In addition, organic modifiers, e.g. methanol, acetonitrile and tetrahydrofuran are used to enhance separations and these increase the affinity of the more hydrophobic analytes for the liquid rather than the micellar phase. The effect of chirality of the analyte on its interaction with the micelles is utilized to separate enantiomers that either are already present in a sample or have been chemically produced. Such pre-capillary derivatization has been used to produce chiral amino acids for capillary electrophoresis. An alternative approach to chiral separations is the incorporation of additives such as cyclodextrins in the buffer solution. [Pg.146]

With capillary electrophoresis (CE), another modern primarily analytically oriented separation methodology has recently found its way into routine and research laboratories of the pharmaceutical industries. As the most beneficial characteristics over HPLC separations the extremely high efficiency leading to enhanced peak capacities and often better detectability of minor impurities, complementary selectivity profiles to HPLC due to a different separation mechanism as well as the capability to perform separations faster than by HPLC are frequently encountered as the most prominent advantages. On the negative side, there have to be mentioned detection sensitivity limitations due to the short path length of on-capillary UV detection, less robust methods, and occasionally problems with run-to-run repeatability. Nevertheless, CE assays have now been adopted by industrial labs as well and this holds in particular for enantiomer separations of chiral pharmaceuticals. While native cyclodextrins and their derivatives, respectively, are commonly employed as chiral additives to the BGEs to create mobility differences for the distinct enantiomers in the electric field, it could be demonstrated that cinchona alkaloids [128-130] and in particular their derivatives are applicable selectors for CE enantiomer separation of chiral acids [19,66,119,131-136]. [Pg.87]

Fillet, M., Hubert, P., Crommen, J. Method development strategies for the enantiosep-aration of drugs by capillary electrophoresis using cyclodextrins as chiral additives. Electrophoresis 1998, 19, 2834—2840. [Pg.208]

Koppenhoeffer, B., Epperlein, U., Zhu, X., Lin, B. Separation of enantiomers of drugs by capillary electrophoresis. IV. Hydroxypropyl-y-cyclodextrin as chiral solvating agent. Electrophoresis 1997, 18, 924—930. [Pg.208]

Liu, L., Nussbaum, M.A. Systematic screening approach for chiral separations of basic compounds by capillary electrophoresis with modihed cyclodextrins. J. Pharm. Biomed. An. 1999,19, 679-694. [Pg.209]

Bergholdt, A.B., Jprgensen, K.W., Wendel, L., Lehmaim, S.V. Fast chiral separations using sulfated fS-cyclodextrin and short-end injection in capillary electrophoresis. [Pg.209]

Perrin, C., Vander Heyden, Y, Maftouh, M., Massart, D.L. Rapid screening for chiral separations by short-end injection capillary electrophoresis nsing highly sulfated cyclodextrins as chiral selectors. Electrophoresis 2001, 22, 3203-3215. [Pg.209]

P. A. (2000). Generic approach to chiral separations chiral capillary electrophoresis with ternary cyclodextrin mixtures. /. Microcol. Sep. 12, 568 — 576. [Pg.142]

Hammitzsch, M., Rao, R. N., and Scriba, G. K. E. (2006). Development and validation of a robust capillary electrophoresis method for impurity profiling of etomidate including the determination of chiral purity using a dual cyclodextrin system. Electrophoresis 27(21), 4334—4344. [Pg.166]

Marini, R. D., Servais, A.-C., Rozet, E., Chiap, P., Boulanger, B., Rudaz, S., Crommen, J., Hubert, P., and Fillet, M. (2006). Nonaqueous capillary electrophoresis method for the enantiomeric purity determination of 5-timolol using heptakis(2,3-di-0-methyl-6-0-sulfo)-p-cyclodextrin validation using the accuracy profile strategy and estimation of uncertainty. /. Chromatogr. A 1120(1—2), 102-111. [Pg.166]

Schmitt, U., Ertan, M., and Holzgrabe, U. (2004). Chiral capillary electrophoresis facts and fiction on the reproducibility of resolution with randomly substituted cyclodextrins. Electrophoresis 25, 2801-2807. [Pg.256]

Gong, Z. L., Zhang, Y., Zhang, H., and Cheng, J. K. (1999). Capillary electrophoresis separation and permanganate chemiluminescence on-line detection of some alkaloids with beta-cyclodextrin as an additive.. Chromatogr. A 855, 329—335. [Pg.307]


See other pages where Cyclodextrins capillary electrophoresis is mentioned: [Pg.266]    [Pg.299]    [Pg.406]    [Pg.406]    [Pg.407]    [Pg.424]    [Pg.430]    [Pg.433]    [Pg.434]    [Pg.55]    [Pg.144]    [Pg.201]    [Pg.208]    [Pg.208]    [Pg.208]    [Pg.208]    [Pg.208]    [Pg.143]    [Pg.143]    [Pg.256]    [Pg.307]   
See also in sourсe #XX -- [ Pg.246 ]

See also in sourсe #XX -- [ Pg.1555 , Pg.1556 , Pg.1557 , Pg.1558 ]




SEARCH



Cyclodextrins in capillary electrophoresis

© 2024 chempedia.info