Apoptosis TUNEL staining

Maier et al., 1998 SD rats MCAo 2 h, with reperfusion 30, 33, and 37, during first 30 min, 1 h, or 2 h of ischemia Infarct size, neurologic function, apoptosis (TUNEL stain, morphology, DNA fragmentation), inflammation (MPO stain) 1 d and 3 d postreperfusion Reduced infarct size, improved neurologic function, reduced apoptosis and inflammation with 1 h or 2 h hypothermia... 

The cardioprotective effect of EPO has also been demonstrated in animal models. EPO administration at the time of reperfusion significantly reduced infarct size and apoptosis (assessed by TUNEL staining) in a rabbit model of acute coronary occlusion and reperfusion.36 This effect was accompanied by increased activation of ERK and Akt in the unstressed myocardium.36 Similarly, EPO administration throughout the period of low-flow ischemia reduced apoptosis and improved recovery of left ventricular pressure in perfused rat hearts.34... 

Yabu, T., S. Todoriki and M. Yamashita. Stress-induced apoptosis by heat shock, UV and y-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining. Fish. Sci. 

Differentiated cells exposed to O.I-lOO fiM chlorpyrifos oxon or 1-100 pjVf trichloropyridinol show increased apoptosis in the absence of NGF addition of NGF protects against apoptosis induced by <100 xM trichloropyridinol or <1 iiAf chlorpyrifos oxon results from cell death ELISA assays confirmed by TUNEL staining for 1(X) piW chlorpyrifos and 1 pW trichloropyridinol. Results suggest that NGF withdrawal renders differentiated cells more vulnerable than undifferentiated cells to OP-induced apoptosis. 

Seven-week-old Wistar rats at normal iodine basehne were divided into four different groups and fed on water of normal, 1.5-fold, 3-fold and 6-fold iodine levels for 8 months. Transmission electron microscope, flow cytometry, DNA quantitation and annexin V early apoptosis detection technique were combined to evaluate intracellular ROS level expression of apoptosis-related molecules (Fas, FasL, bcl-2, bax) was traced by indirect fluorescent stained flow cytometry and immunohistochemistry qualitative and quantitative analyses of cell apoptosis were performed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining. 

Su and coworkers investigated the induction of apoptosis by Tan-I at 1-10 pg/mL in human colon cancer Colo 205 cells. Tan-I reduced cell growth in a concentration-dependent manner, inducing apoptosis accompanied by an increase in TUNEL-stained cells in the sub-Gl fraction. The treatment with Tan-I at 0, 1, 2.5, 5, and 10 pg/mL for 72 h increased the percentage of cells in sub-Gl phase from 3.83% to 7.22%, 8.68%, 14.4%, and 32.98%, respectively. The expression of p53, p21, bax, and caspase-3 increased in Tan-I-treated cells. The authors suggested that Tan-I induces apoptosis in Colo 205 cells through both mitochondrial-mediated intrinsic ceU-death pathways and p21-mediated GO/Gl cell cycle arrest [50]. 

Furthermore, since the cell growth arrest is often linked to cell death. The annexin V staining positive cell or the amount of DNA fragmentation assessed by TUNEL and FACS analysis has been interpreted as indicative of apoptosis. The HDACI-induced apoptosis can also be determined by Western blotting of target proteins, detection of mitochondrial membrane potentials, activation of caspases and their substrate cleavages in a dose- and time-dependent manner. 

FIGURE 8.5 Proapoprotic experimental therapies in pulmonary arterial hypertension (PAH). A common denominator of these therapies is the induction of apoptosis as well as suppression of proliferation in the pulmonary artery wall. On the left are untreated rats with monocrotaline-induced PAH and on the right are rats treated with elastase inhibitors. Increased apoptosis of the intima-media is showed by TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling), and decreased proliferation is imaged by staining for proliferation cell nuclear antigen (PCNA). The same pattern has been shown in the media of treated rats by all the therapies shown in the box. (From Ref. 116, with permission.)... 

To explore the contribution of resveratrol to the proliferation and apoptosis of lens epithelial cells, the thiazolyl tetrazolium (MTT) assay was conducted [47]. The stilbene effects on the proliferation of lens epithelial cells cultured with different concentrations of this compound were measured. HE staining, transmission electron microscopy (TEM), TUNEL fluorescence staining, and the aimexin V assay... 

Fig. 1. Apoptosis in the rhombencephalic neural crest of the chick embryo as revealed by acridine orange staining (A,B) and TUNEL (C,D). The embryos shown in (A) and (C) are at stage 10, but those in (B) and (D) are older, stage 11 (see Note 1 and color plate 3 appearing after p. 368). (See Color Plate)...

Fig. 1C,D shows the results obtained using TUNEL, and streptavidin-horserad-ish peroxidase detection, on whole chick embryos during the period of hindbrain neural crest apoptosis. As was found with the acridine orange staining, during the early phase of crest production, there is staining over rhombomere 3 (Fig. 1C), and... 

Lovastin induced apoptosis in acute lung injury and idiopathic pulmonary fibrosis fibroblasts as determined by TUNEL, acridine orange staining, and flow cytometry (Tan et al. 1997). 

Daniel, B. and DeCoster, M. A. (2004). Quantification of sPLA2-induced early and late apoptosis ehanges in neuronal eell cultures using combined TUNEL and DAPI staining. Brain Res. 

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