Acridine orange stain

Ratinaud MH, Leprat P, Julien R. 1988. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry 9 206-212. 

Another important limitation to be aware of is that the induction of apoptosis in a population of cells will not always result in a clearly distinct subdiploid peak (12), We have seen this particularly when apoptosis is induced in Burkitt lymphoma cells by serum deprivation. After approx 2 wk, all cells are apoptotic as can be clearly seen by the two-dimensional light scatter assay and confirmed by microscopic analysis of acridine orange-stained cells, but the cell-cycle profile gives the appearance of a mainly viable population (Fig. 5). 

A 65-year-old man with bilateral osteoarthritis of the knees developed an effusion in the left knee. The swollen joint was treated with an intra-articular injection of triamcinolone hexacetonide 40 mg. The next day, he developed acute arthritis in the injected knee the joint was swollen and tender and he was unable to walk. Examination of the joint fluid showed 35 ml of a thick, turbid, yellowish synovial fluid with a leukocyte count of 13 x 106/1 (95% neutrophils). Gram and acridine orange stains were negative. Wet preparations of... 

Remove the covering dish and add rapidly a suspension of L. monocytogenes (acridine orange stained, 200 pL of 10 °cfu ml in MOPS buffer (50 ml) and continue stirring for 3 min. 

Acridine orange staining has the advantages that it is extremely rapid, cheap, and easy to perform. On the other hand, it has a number of disadvantages. Firstly, because it is used vitally, and since the dye is not fixable and consequently the stain is transient, the embryos cannot be processed for other detection procedures,... 

Fig. 1. Apoptosis in the rhombencephalic neural crest of the chick embryo as revealed by acridine orange staining (A,B) and TUNEL (C,D). The embryos shown in (A) and (C) are at stage 10, but those in (B) and (D) are older, stage 11 (see Note 1 and color plate 3 appearing after p. 368). (See Color Plate)...

Fig. 1C,D shows the results obtained using TUNEL, and streptavidin-horserad-ish peroxidase detection, on whole chick embryos during the period of hindbrain neural crest apoptosis. As was found with the acridine orange staining, during the early phase of crest production, there is staining over rhombomere 3 (Fig. 1C), and... 

Lovastin induced apoptosis in acute lung injury and idiopathic pulmonary fibrosis fibroblasts as determined by TUNEL, acridine orange staining, and flow cytometry (Tan et al. 1997). 

Cells separated by filtration from large aliquots of water can be visualized and counted on a 0.25 xm filter using the epifluorescent technique and a stain such as acridine orange. Stains such as fluorescein diacetate, 5-cyano-2,3-ditolyltetrazolium chloride or p-iodonitrotetrazolium violet indicate active metabolism by the formation of fluorescent products [9]. Antibody fluorescence microscopy is similar to general fluorescent microscopy, except that the fluorescent dye used is bound to antibodies specific to SRBs. Only bacteria recognized by the antibodies fluoresce. Results can be analyzed within two hours. The technique detects viable and nonviable bacteria but it is limited to the type of SRB used in the manufacture of these antibodies [19]. 

Meseguer, M. de Rafael, L. Baquero, M. Martinez, F. M Lopez-Brea, M. Acridine orange stain in the early detection of bacteria in blood cultnres. Eur. J. Clin. Microbiol. 1984, 3, 113-115. 

Sciotto, C. G. Lauer, B. A. White, W. L. Istre, G. R. Detection of Borrelia in acridine orange-stained blood smears by fluorescence microscopy. Arch. Pathol. Lab. Med. 1983, 107, 384-386. 

Bartzatt, R. Acridine orange staining I. In vitro derived cells lines. J. Histotechnol. 1987, 10, 91-93. 

Polard, T. Jean, S. Medina, G. Laplanche, C. Pinelli, E. Gauthier, L. Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring. Ecotoxicol. Environ. Saf. 2011, 74,144-149. 

McCarthy, L. R. Senne, J. E. Evaluation of acridine orange stain for detection of microorganisms in... 

Freedman, D. O. Berry, R. S. Rapid diagnosis of Bancroftian filariasis by acridine orange staining of centrifuged parasites. Am. J. Prop. Med. Hyg. 1992, 47, 787-793. 

Wongsrichanalai, C. Webster, H. K. Brown, A. E. Rapid diagnosis of malaria by acridine orange staining of centrifuged parasites. Lancet 1989, 1, 967. 

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