TUNEL staining

Fig. 3A-D Postischemic damage in CA1 on day 4. A Staining for the TUNEL assay showing numerous positive cells. B Statistical analysis of TUNEL+ cells per frame of 0.4 mm2 in CA1 p < 0.001, one-way ANOVA followed by Tukey-Kramer post hoc. C Staining for Fluoro-Jade demonstrating similar pattern to the TUNEL stain. D Statistical analysis of Fluoro-Jade+ cells per frame of 0.4 mm2 in CA1 p < 0.001, one-way ANOVA followed by Tukey-Kramer post hoc. Scale bar = 200 pm...

Maier et al., 1998 SD rats MCAo 2 h, with reperfusion 30, 33, and 37, during first 30 min, 1 h, or 2 h of ischemia Infarct size, neurologic function, apoptosis (TUNEL stain, morphology, DNA fragmentation), inflammation (MPO stain) 1 d and 3 d postreperfusion Reduced infarct size, improved neurologic function, reduced apoptosis and inflammation with 1 h or 2 h hypothermia... 

Figure 29.8. Kidney tissue from a rat treated with para-aminophenol (300mg/kgip). Tissue was stained with hematoxylin and eosin and was subjected to terminal deoxynucleotidyl transferase-diaminobenzidine (TUNEL) staining to reveal DNA strand breaks. Darker gray areas represent tubules that stain positive for DNA strand breaks. Final magnification was lOOx (left panel) and 360x (right panel).

ISEL- or TUNEL-stained samples will make the microscopic assessment easier and perhaps more objective, particularly if the observer is not an experienced microscopist. On the other hand, these methods may underestimate the... 

The cardioprotective effect of EPO has also been demonstrated in animal models. EPO administration at the time of reperfusion significantly reduced infarct size and apoptosis (assessed by TUNEL staining) in a rabbit model of acute coronary occlusion and reperfusion.36 This effect was accompanied by increased activation of ERK and Akt in the unstressed myocardium.36 Similarly, EPO administration throughout the period of low-flow ischemia reduced apoptosis and improved recovery of left ventricular pressure in perfused rat hearts.34... 

Yabu, T., S. Todoriki and M. Yamashita. Stress-induced apoptosis by heat shock, UV and y-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining. Fish. Sci. 

Differentiated cells exposed to O.I-lOO fiM chlorpyrifos oxon or 1-100 pjVf trichloropyridinol show increased apoptosis in the absence of NGF addition of NGF protects against apoptosis induced by <100 xM trichloropyridinol or <1 iiAf chlorpyrifos oxon results from cell death ELISA assays confirmed by TUNEL staining for 1(X) piW chlorpyrifos and 1 pW trichloropyridinol. Results suggest that NGF withdrawal renders differentiated cells more vulnerable than undifferentiated cells to OP-induced apoptosis. 

Seven-week-old Wistar rats at normal iodine basehne were divided into four different groups and fed on water of normal, 1.5-fold, 3-fold and 6-fold iodine levels for 8 months. Transmission electron microscope, flow cytometry, DNA quantitation and annexin V early apoptosis detection technique were combined to evaluate intracellular ROS level expression of apoptosis-related molecules (Fas, FasL, bcl-2, bax) was traced by indirect fluorescent stained flow cytometry and immunohistochemistry qualitative and quantitative analyses of cell apoptosis were performed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining. 

Su and coworkers investigated the induction of apoptosis by Tan-I at 1-10 pg/mL in human colon cancer Colo 205 cells. Tan-I reduced cell growth in a concentration-dependent manner, inducing apoptosis accompanied by an increase in TUNEL-stained cells in the sub-Gl fraction. The treatment with Tan-I at 0, 1, 2.5, 5, and 10 pg/mL for 72 h increased the percentage of cells in sub-Gl phase from 3.83% to 7.22%, 8.68%, 14.4%, and 32.98%, respectively. The expression of p53, p21, bax, and caspase-3 increased in Tan-I-treated cells. The authors suggested that Tan-I induces apoptosis in Colo 205 cells through both mitochondrial-mediated intrinsic ceU-death pathways and p21-mediated GO/Gl cell cycle arrest [50]. 

Figure 13.6 (See color insert) Increased tumor uptake of [ In]DTPA-PEG-annexin V. Note the high correlation of tumor uptake of the radiotracer with the apoptotic index (left) and TUNEL staining. This data is reproduced with permission from Ref. (29).

Protocol 12.1 Dissection Techniques, 203 Protocol 12.2 Antibody Staining, 207 Protocol 12.3 (3-calactosidase Detection, 212 Protocol 12.4 BrdU Labeling, 215 Protocol 12.5 In Situ Hybridization, 216 Protocol 12.6 Cobalt Sulfide, 220 Protocol 12.7 Acridine Orange, 221 Protocol 12.8 TUNEL Staining, 222 Protocol 12.9 Phalloidin, 224 Protocol 12.10 DAPI, 225... 

TUNEL staining cell-death analysis (fixed tissue)... 

Fig. 3 (continued) assayed by TUNEL. Numerous TUNEL-positive cells were observed after treatment with gpl 20, while almost no apoptotic cells were seen in cultures that were not incubated with gpl 20 (p<0.001 serum-free media (control) vs. 200 ng/ml gpl 20 in serum-free media for 24 h). Almost all occludin-positive cells were TUNEL positive for 200 ng/ml gpl 20. Control with buffer only and no enzyme showed no TUNEL staining (not shown), (b) After intra-caudate-putamen (CP) injection of HIV-1 500 ng gpl 20, fewer occludin-positive structures were seen, (c) Decrease in the number of claudin-5 (a tight junction protein)-positive structures parallels the leakage of Evans blue (EB) in CP previously injected with HIV-1 gpl 20. (d) Reduction in the number of laminin (a basement membrane proteinj-positive structures observed after intra-CP injection of HIV-1 gpl 20, suggesting a degradation of the basement membrane protein (modified from [105])... 

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