Adenovirus cells infected with

Furthermore, infection of adult rat myocytes with adenoviral vectors containing either wild-type or L39stop PLN cDNAs indicated that wild type PLN decreased the contractile parameters and calcium kinetics, compared to control cells infected with an adenovirus expression GFP. However, the PLN-L39stop did not alter myocyte mechanics or calcium cycling. 

TNF-a, IL-12, and GMCSF (CaudeU et aL, 2002). However, when IL-24 is over expressed via an adenovirus gene delivery system, it selectively induces apoptosis of cancer cells but not normal cells (Madireddi et at, 2000 Mhashilkar et at, 2001). The mechanisms that distinguish the different biological activities of lL-24 are currently under intense investigation. Finally, lL-26 was identified as a molecule secreted by T-cells infected with herpesvirus saimiri (Knappe et at, 2000). lL-26 has been shown to be up-regulated in NK and T cells by 1L-2/1L-12 and anti-CD3 antibody. However, the specific functional properties of lL-26 have not been reported. 

The isolation and characterization of adenovirus mRNA from cells infected with adenovirus type 2 was described [114]. Purification of the poly(A) containing mRNA was achieved on a poly(U)-Sepharose column and the mRNA was separated from 95% of the rRNA and tRNA. Poly(U)-Sepharose has also been used to separate mRNA from polysomes of KB cells, and recoveries of 90% were reported [115]. 

Azaguanine-resistant avian sarcoma virus-infected cells were less tu-morigenic than nonresistant cells in hamsters. Incubation of cells (infected with adenoviruses in tissue culture) with 8-azaguanine (10 M) inhibited reproduction of the virus. At this concentration, the drug was not toxic for healthy mammalian cells. ... 

McIntosh, K., Payne, S., and Russell, W. C., 1971, Studies on lipid metabolism in cells infected with adenovirus, J. Gen. Virol. 10 251. 

More recent observations have suggested that adenovirus infection specifically inhibits one or more cellular DNases. In cells infected with U2ts, a DNA-negative early mutant, at a nonpermissive temperature, both viral and cellular DNA become considerably more degraded than in corresponding wild-type infections (D Halliun et al., 1981). Somewhat enhanced degradation of viral DNA was also ob-... 

Burlingham, B. T., and Doerfler, W., 1972, An endonuclease in cells infected with adenoviruses and associated with adenovirions. Virology 48 1. 

Friefeld, B. R., Lichy, J. H., Hurwitz, J., and Horwitz, M. S., 1983, Evidence for an altered adenovirus DNA polymerase in cells infected with the mutant H5tsl49, Proc. Natl. Acad. Sci. USA 80 1589. 

Ginsberg, H. S., Bello, L. J., and Levine, A. J., 1967, Control of biosynthesis of host macromolecules in cells infected with adenovirus, in The Molecular Biology of Viruses (J. S. Colter, and W. Paranchych, eds.), p. 547, Academic Press, New York. 

Ross, S. R., Levine, A. J., Galos, R. S., Williams, J., and Shenk, T., 1980, Early viral proteins in HeLa cells infected with adenovirus type 5 host range mutants. Virology 163 475. 

Soderlund, H., Pettersson, U., Vennstromm, B., Philipson, L., and Mathews, M. B., 1976, A new species of virus-coded, low molecular weight RNA from cells infected with adenovirus type 2, Cell 7 585. 

Takahashi, M., Ogino, T., Baba, K., and Onaka, M., 1969, Synthesis of deoxyribonucleic acid in human and hamster kidney cells infected with human adenovirus types 5 and 12, Virology 37 513. 

Pereira, H. G., 1960, A virus inhibitor produced in HeLa cells infected with adenovirus, Virology 11 590. 

Recombinant adenoviruses are versatile tools for gene delivery into mammalian tissue-culture cells. Adenoviruses can infect both dividing and nondividing cells, with efficiencies approaching 100%. Here we describe the use of adenoviruses to express reporter proteins in high-throughput cell-based assays. 

Fig. l.A sample plate from a screen to identify small molecules that upregulate the expression of a cyclin-luciferase reporter protein. HeLa cells were infected with an adenovirus expressing cyclin-luciferase, as described in the methods section. The graph shows the activity of all wells from a single 384-well plate. Several compounds that upregulate cyclin-luciferase expression were identified. 

Other protocols involve cell lines with integrated rep/cap cassettes (Clark et al., 1995 Gao et al., 1998 Liu et al., 1999 Chadeuf et al., 2000 Mathews et al., 2002 Qiao et al., 2002a,b) infected with adenovirus or, alternatively, a recombinant herpesvirus system has been used to provide both helper virus function and rep/cap (Conway et al., 1997, 1999). In a switch away from using mammalian cell and helper virus production systems, rAAV vectors have been made in insect cells where the AAV genes are expressed under the control of insect promoters and the traditional helper virus gene products are not required (Urabe et al., 2002). Stable producer cell... 

Adeno-associated virus contains linear single-stranded DNA genome comprising only rep and cap genes non-immunogenic replication requies co-infection with lack of suitable packaging cell lines lowtiters (105—106) virus particles/ml) small DNA insertion capacity ( 4.7kb) adenovirus or other viruses... 

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