Adenovirus gene delivery

TNF-a, IL-12, and GMCSF (CaudeU et aL, 2002). However, when IL-24 is over expressed via an adenovirus gene delivery system, it selectively induces apoptosis of cancer cells but not normal cells (Madireddi et at, 2000 Mhashilkar et at, 2001). The mechanisms that distinguish the different biological activities of lL-24 are currently under intense investigation. Finally, lL-26 was identified as a molecule secreted by T-cells infected with herpesvirus saimiri (Knappe et at, 2000). lL-26 has been shown to be up-regulated in NK and T cells by 1L-2/1L-12 and anti-CD3 antibody. However, the specific functional properties of lL-26 have not been reported. 

Tallone T, Malin S, Samuelsson A Wilbertz J, Miyahara M, Okamoto K, Poellinger L, Philipson L, Pettersson S. A mouse model for adenovirus gene delivery. Proc Natl Acad Sci USA 2001 98(14) 7910-7915. 

S. S. Diebold, H. Lehrmann, M. Kursa, E. Wagner, M. Cotten, and M. Zenke, Efficient gene delivery into human dendritic cells by adenovirus polyethyleni-mine and mannose polyethylenimine transfection, Hum. Gene Ther., 10 (1999) 775-786. 

Curiel DT, Agarwal S, Wagner E, et al. Adenovirus enhancement of transfer-rin-polylysine-mediated gene delivery. Proc Natl Acad Sci USA 1991 88(19) 8850-8854. 

Marshall, D.J., Palasis, M., Lepore, J.J. and Leiden, J.M. (2000) Biocompatibility of cardiovascular gene delivery catheters with adenovirus vectors An important determinant of the efficiency of cardiovascular gene transfer. Mol. Then, 1,423 129. 

Wirtz, S., P.R. Galle, and M.F. Neurath. 1999. Efficient gene delivery to the inflamed colon by local administration of recombinant adenoviruses with normal and modified fibre structure. Gut 44 800. 

Bennett, J., Zeng, Y., Bajwa, R., Klatt, L., Li, Y., and Maguire, A.M. (1998). Adenovirus-mediated delivery of rhodopsin-promoted bcl-2 results in a delay in photoreceptor cell death in the rd/rd mouse. Gene Ther. 5 1156-1164. 

Recombinant adenoviruses are versatile tools for gene delivery into mammalian tissue-culture cells. Adenoviruses can infect both dividing and nondividing cells, with efficiencies approaching 100%. Here we describe the use of adenoviruses to express reporter proteins in high-throughput cell-based assays. 

R. D. Alvarez, G. P. Siegal, J. T. Douglas, and D. T. Curiel, Basic fibroblast growth factor enhancement of adenovirus-mediated delivery of the herpes simplex virus thymidine kinase gene results in augmented therapeutic benefit in a murine model of ovarian cancer, Clin. Cancer Res. 4 2455 (1998). 

Yang, B., Ma, T., Dong, J. Y. and Verkman, A. S. (2000). Partial correction of the urinary concentrating defect in aquaporin-1 null mice by adenovirus-mediated gene delivery. Hum. Gene Ther. 11, 567-575. 

Ghadge, G. D. et al. (1995). CNS gene delivery by retrograde transport of recombinant replication-defective adenoviruses. Gene Ther. 2(2), 132-137. 

Another viral-based gene delivery system that has been extensively studied is the adenovirus (Ad) vector. One of the first in vivo Ad-mediated gene transfer experiments was performed with the use of... 

Wagner, E., Zatloukal, K., Cotten, M., Kirlappos, H., Mechtler, K., Curiel, D.T., and Bimstiel, M.L. (1992). Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc. Natl. Acad Sci. USA 89, 6099 6103. 

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