V79 hamster cells

Leukotrienes. Leukotrienes, products of the Hpoxygenase pathway, are generally less radioprotective than the PGs, with the exception of LTC4, which is among the most potent of the naturally occurring eicosanoids (214). LTC radioprotects V79 hamster cells in vitro and mouse CEU-S and... 

Figure 1 Absorbed dose necessary to produce a given biological effect on a given system, in the present case 50% inactivation of V79 hamster cells in exponential growth phase. The dose needed depends on the type of the particles (photons, alpha particles, or uranium ions) and on their energy. (From Ref 15.)...

Slamenova, D., Dusinska, M., Bastlova, T. Gabelova, A. (1990) Differences between survival, mutagenicity and DNA replication in MMS- and MNU-treated V79 hamster cells. Mutat. Res., 228, 97-103... 

Besides expression in insect cells and bacteria (E. coli) a variety of other expression systems are used like mammalian cells, V79 hamster cells, systems in yeast (Friedberg 1999 and references cited therein). High expression level and/or high yields in producing cells are achieved in baculovirus and E. coli. Therefore, these systems are nowadays in broad application in the industrial environment, also because of their commercial availability. 

BCNU was mutagenic in vitro (Ames assay, human lymphoblast HGPRT assay) and clastogenic in vitro (V79 hamster cell micronucleus assay). 

Bartsch, H., C. Malaveille, A.M. Camus, G. Martel-Planche, G. Brun, A. Hautefeuille, N. Sabadie, A. Barbin, T. Kuroki, C. Drevon, C. Piccoli, and R. Montesano Validation and comparative studies on 180 chemicals with S. typhimurium strains and V79 hamster cells in the presence of various metabolizing systems Mutation Res. 76 (1980) 1-50. 

Boronated nucleosides bearing a cobaltacarborane group (e.g., 42) have also been synthesized. Compound 42 was found to have low cytotoxicity and to readily accumulate within V79 hamster cells. 

Romert, L. and Jenssen, D. (1983). Rabbit alveolar macrophage-mediated mutagenesis of polycyclic aromatic hydrocarbons in V79 Chinese hamster cells. Mutat. Res. Ill, 245-252. 

DMT, TMT, DBT, TBT and DPhT chlorides exhibited in vitro spindle disturbance in V79 Chinese hamster cells of brain tubulin. The V79 cells lose stainable spindles at higher concentration. The cell mitosis activity effect at low concentration increased with the lipophilicity of the OTC, but all compounds showed a concentration dependence on microtubules. The OTC seem to act through two different cooperative mechanisms inhibition of microtubule assembly and interaction with hydrophobic sites. The latter mechanism might involve Cl/OH ion exchange28. 

Jansson, K, and V. Jansson. 1991. Induction of mutation in V79 Chinese hamster cells by tetrahydroquinone, a metabolite of pentachlorophenol. Mutat. Res. 260 83-87. 

Thust, R., Mendel, J., Schwarz, H. and Warzoki, R. (1980). Nitrosated urea pesticide metabolites and other nitrosamides. Activity in clastogenicity and SCE assays, and aberration kinetics in Chinese hamster V79-E cells. Mutation Res. 79 239-248. 

V79 Chinese hamster cells Gene mutation + - Paschin and Bahitova 1982... 

Paschin YV, Bahitova LM. 1982. Mutagenicity of benzo[a]pyrene and the antioxidant phenol at the HGPRT locus of V79 Chinese hamster cells. Mutat Res 104 389-393. 

Escherichia coli WP2 uvrA, WP2, other strains, reverse mutation DNA strand breaks, cross-links or related damage, Chinese hamster V79 lung cells in vitro°... 

The metabolism of 7V-nitrosodiethanolamine by a-hydroxylation, which is a cytochrome P450 (CYP)-mediated pathway, was not detected in liver preparations from uninduced male Fischer 344 rats (Farrelly et al., 1984,1987). The existence of a-hydroxylation was proved later, notably by the formation of glycol aldehyde in liver microsomes from rats pretreated with CYP2E1 inducers. The microsomal metabolism of N-nitrosodiethanolamine was slower by a-oxidation than by [3-oxidation (Loeppky, 1999). Bioactivation tests of jV-nitrosodiethanolamine in V79 Chinese hamster cells showed that cytotoxicity was observed only in cells transfected with human CYP2E1 but not in cells expressing CYP2B1 or in the controls (Janzowski et al., 1996 Loeppky, 1999). 

In mammalian assays, glycidol has been tested in human lymphocytes and Chinese hamster cells in vitro for induction of chromosomal aberrations and sister chromatid exchanges. It was also tested in vivo in the mouse micronucleus assay. All test results were positive, as were those of gene mutation assays using Chinese hamster V79 cells and mouse lymphoma L5178Y cells. An in-vivo assay to detect chromosomal aberrations in mouse bone-marrow cells gave negative results. 

Table 1.3—Mutagenic potency of various chemicals in V79 Chinese hamster cells ...

Kuroki, T. Munakata, K. (1985) Assays for the induction of mutations to ouabain resistance in V79 Chinese hamster cells in culture with cell- or microsome-mediated metabolic activation. Prog. Mutat. Res., 5, 543-545... 

Dimethylformamide inhibited intercellular communication (as measured by metabolic co-operation) between Chinese hamster V79 hprt cells. 

Ellard, S. Parry, E.M. (1993) Induction of micronuclei in V79 Chinese hamster cells by hydroquinone and econazole nitrate. Mutat. Res.. 287, 87-91... 

In addition to genotoxicify studies with chlorophenols themselves, the corresponding activity of some of their metabolites has also been examined. The major metabolite of pentachlorophenol in mice and rats, tetrachloro-7 ora-hydroquinone, induced mutations in the hprt locus (but not the ouabain-resistance locus) of V79 Chinese hamster cells (Jansson Jansson, 1991), covalent damage (including 8-hydroxyguanine) in naked DNA in the presence of Cu(Il) (Naito et al., 1994), DNA strand breaks and accumulation of p53 in NIH 3T3 cells in vitro and transfonnation of mouse embryo fibroblasts in a two-stage model (Wang et al., 1997). The same metabolite, as well as tetrachloro-1,4-benzoquinone and tetrachloro-l,2-benzoquinone, caused DNA strand breaks in V79 Chinese hamster cells (Dahlhaus et al., 1996). 

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