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Hgprt assay

Lee CG, Webber TD. 1985. The induction of gene mutations in the mouse lymphoma L5178Y/K+/ assay and the Chinese hamster V79/HGPRT assay. In Ashby J, de Serres FJ, et al, eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers, 547-554. [Pg.112]

Procedure for the Chinese Hamster V79/Hgprt Assay. The assay usually comprises three test concentrations, each in duplicate, and four vehicle control replicates. Suitable positive controls are ethylmethane sulphonate (—S9) and dimethyl benzanthracene (+S9). V79 cells with a low nominal passage number should be used from frozen stocks to help minimize genetic drift. The procedure described includes a reseeding step for mutation expression. [Pg.208]

Demarini DM, Brimer PA, Hsie AW. 1984. Cytotoxicity and mutagenicity of coal oils in the CHO/HGPRT assay. Environ Mutagen 6(4) 517-527. [Pg.172]

BCNU was mutagenic in vitro (Ames assay, human lymphoblast HGPRT assay) and clastogenic in vitro (V79 hamster cell micronucleus assay). [Pg.220]

Chinese Hamster CHO/Hgprt System. Chinese hamster ovary (CHO) cells have 21 or 22 chromosomes with one intact X chromosome and a large acrocentric marker chromosome (Natarajan and Obe, 1982). The use of these cells in mammalian mutation experiments was first reported by Hsie et al. (1975), and was refined into a quantitative assay for mutagenicity testing by O Neill. The performance of this system has been reviewed by the USA EPA Gene-Tox Program. The experimental procedure for this assay is similar to the V79/Hgprt system already described, and for more detailed descriptions the reader is referred to Li et al. (1987). [Pg.209]

Oberly, T.J., Bewsey, B.J. and Probst, G.S. (1987). A procedure for the CHO/HGPRT mutation assay involving treatment of cells in suspension culture and selection of mutants in soft agar. Mutation Res. 182 99-111. [Pg.233]

Yang LL. 1988. CHO/HGPRT mutation assay. Study No. T5196.332. Microbiological Associates Inc., Bethesda, MD. [Pg.200]

Dichlorophenol with or without metabolic activation did not induce an increase in mutagenic response in the Chinese hamster ovary HGPRT forward mutation assay (Litton Bionetics 1986a). This compound was also inactive in the Balb/3T3 in vitro transformation assay (Litton Bionetics 1985). [Pg.100]

Litton Bionetics. 1986a. Mutagenicity evaluation of 2,5-dichlorophenol in the CHO HGPRT forward mutation assay. Report to Bayer AG Institut Fuer Toxicology, West Germany, by Litton Bionetics, The Netherlands. [Pg.255]

The mouse lymphoma assay is in fact just one of several mammalian cell assays designed to determine increases in mutation rate. It focuses on the thymidine kinase (tk) assay in murine lymphoma cells (L5178Y), though tk data have also been produced from the human lymphoblastoid cell line TK6, and at the hgprt (hypox-anthine-guanine phosphoribosyl-transferase) locus in Chinese Hamster ovary or lung (V79) cells and mouse lymphoma cells. Since Ames mutation data and often... [Pg.254]

Fox, M. Delow, G.F. (1985) Tests for mutagenic activity at the HGPRT locus in Chinese hamster V79 cells in culture. In Ashby, J., de Serres, F.J., Draper, M., Ishidate, M., Jr, Margolin, B.H., Matter, B.E. Shelby, M.D., eds, Progress in Mutation Research, Volume 5, Evaluation of Short-Term Tests for Carcinogens. Report of the International Programme on Chemical Safety s Collaborative Study on in vitro assays, Amsterdam, Elsevier Science, pp. 517-523... [Pg.308]

O Neill, J.P. and Hsie, A.W. (1979). "The CHO/HGPRT mutation assay Experimental procedure, pagg 65 in Banbury Report 2, Mammalian Cell Mutagenesis The Maturation of Tkst Systems, HsiE, A.W., O Neill, J.P., AND McElheny, V.K., Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New Yrrk). [Pg.150]

Moore, M.M., Parker, L., Huston, J., Harrington-Brock, K. Dearfield, K.L. (1991) Comparison of mutagenicity results for nine compounds evaluated at the hgprt locus in the standard and suspension CHO assays. Mutagenesis, 6, 77-85... [Pg.1076]

Hirsch, and A.W. Hsie. A quantitative assay of mutation induction at hypoxanthine-guanine phospho-ribosyl transferase locus in Chinese hamster ovary cells (CHO/HGPRT system) Development and definition of the system. Mutat. Res. 45 91-101, 1977. [Pg.279]

Prenatal and postnatal development none Genetic toxicology0 Ames test, human lymphocyte chromosomal aberration assay, CHO/HGPRT gene mutation assay, mouse micronucleus assay Carcinogenicity none... [Pg.932]

Not genotoxic (Ames, CHO/ HGPRT forward point mutation assay, in vitro chromosomal aberrations in human PBLs)... [Pg.1051]

In in vitro (bacterial reverse mutation, CHO/ HGPRT forward mutation, and rat lymphocyte chromosomal aberration assays) and in vivo (mouse bone marrow micronucleus assay) tests, fexofenadine hydrochloride revealed no evidence of mutagenicity. [Pg.1144]

O Neill JP and Hsie AW (1979) The CHO/HGPRT mutation assay experimental procedure. In Hsie AW, O Neill JP, and McElhenny VK (eds.) Mammalian Cell Mutagenesis The Maturation of Test Systems, Banbury Report No. 2, pp. 55-70, 311-318, 407-420. Cold Spring Harbor, NY Cold Spring Harbor Press. [Pg.1243]

It was positive for gene mutations (HGPRT and TK genes) as well as for chromosomal aberrations, sister chromatid exchange, and DNA repair-deficient bacterial assays. [Pg.1685]

See other pages where Hgprt assay is mentioned: [Pg.59]    [Pg.312]    [Pg.1674]    [Pg.806]    [Pg.109]    [Pg.143]    [Pg.23]    [Pg.173]    [Pg.59]    [Pg.312]    [Pg.1674]    [Pg.806]    [Pg.109]    [Pg.143]    [Pg.23]    [Pg.173]    [Pg.193]    [Pg.178]    [Pg.134]    [Pg.140]    [Pg.141]    [Pg.113]    [Pg.133]    [Pg.263]    [Pg.198]    [Pg.387]    [Pg.97]    [Pg.549]    [Pg.54]    [Pg.601]    [Pg.304]    [Pg.691]    [Pg.1146]    [Pg.1239]   
See also in sourсe #XX -- [ Pg.208 , Pg.209 ]



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