Ulex Europaeus Lectin

The expression of many endothelial markers including CD34, factor VIII, CD31, or Ulex europaeus lectin is fairly weak in normal vasculature, whereas it can become quite prominent in pathologic conditions such as chronic liver disease and hepatocellular carci-... 

Affinity chromatography has been used to purify Ulex europaeus lectin II.The lectin precipitates to different extents with blood group Ai, A2, B, H, Le , Le , and I precursor substances as well as with milk oligosaccharides. The maximum inhibition of the precipitin reaction occurs with H-specific oligosaccharide and to a lesser extent with the related oligosaccharide (8), 2 -< -Q -L-fucosyl-lactose, and oligomers of 2-acetamido-2-deoxy-D-glucose. 

Glycohybrid of peptido lactam 34 was developed using CuAAC reaction of sugar azide 33 with a-acetamido-a-propargyl-P-lactam 32 (Scheme 6.11). Glycohybrid 35, obtained from P-l-azido-L-fucose, was bound to Ulex Europaeus Lectin-1 (UEL-I) after conformational change around a rotatable triazolylmethylene moiety [27]. 

CD31 (54), Factor Vlll-related antigen (55), and CD34 (56) cannot often be demonstrated in poorly differentiated vascular tumors. The use of the lectin Ulex europaeus agglutinin 1 (UEA-1) (57) is a more sensitive method to detect endothelial cells. Like other lectins, however, UFA-1 reacts with many other cell types. 

It is well known that intestinal M cells have the ability to efficiently transcytose macromolecules and inert particles, which has stimulated researchers to target M cells for oral delivery of macromolecular drugs and particles.26,52 For example, Ulex europaeus 1 (UEAl), a lectin specific for a-L-fucose residues, selectively binds to mouse Peyer s patch M cells.56 It has become apparent that UEAl can be used to target macromolecules to mouse Peyer s patch M cells and to enhance macromolecular... 

Clark, M. A., Jepson, M. A., Simmons, N. L, and Hirst, B. H. Selective binding and transcytosis of Ulex europaeus 1 lectin by mouse Peyer s patch M-cells in vivo. Cell Tissue Res. 282 455-461, 1995. 

Synthesis of Type 2 Human Blood Group Antigenic Determinants. The H, X and Y Haptens and Variations of the H Type 2 Determinants as Probes for the Combining Site of the Lectin 1 of Ulex europaeus, O. Hindsgaul, T. Norberg, J. LePendu, and R. U. Lemieux, Carbohydr. Res., 109 (1982) 109-142. 

Molecular Recognition XI. The Synthesis of Extensively Deoxygenated Derivatives of the H-type 2 Human Blood Group Determinant and their Binding by an Anti-H-type 2 Monoclonal Antibody and the Lectin 1 of Ulex europaeus, U. Spohr, E. Paszkiewicz-Hnatiw, N. Morishima, and R. U. Lemieux, Can. J. Chem., 70 (1992) 254-271. 

The recognition of three different epitopes for the H-type 2 human blood group determinant by lectins of Ulex europaeus, Galactia... 

Ulex lectin UEAI Ulex europaeus a-I.-Fucose... 

Foster, N. Clark, M.A. Jepson, M.A. Hirst, B.H. Ulex europaeus 1 lectin targets microspheres to mouse peyer s patch M-Cells in vivo. Vaccine 1998, 16, 536-541. 

In the same study, intravenous injection of Ulex europaeus 1 lectin microspheres were shown to be specifically taken up by lung endothelial cells and rapidly underwent migration into the extravascular tissues and into the airspace within 5-10 minutes after injection, with 90% of the injected dose being identified in the lung. Intratracheal administration of heparin nanospheres of 200 to 800-nm diameter containing iron oxide (Fe304) and ionic iron (Fe3+) to pentobarbital-anesthetized adult male CBA/J mice indicated a very similar deposition profile to that observed after intravenous injection of amphotericin B-containing nanospheres (Table 3) [138],... 

Other associations have been investigated by the same methods. Lectin UE-I from Ulex europaeus (Section 15.4.3) and PT-II from Psophocarpus tetragonolobus both bind the H-type trisaccharide glycoside a-L-Fuc-(l—>2)-j3-D-Gal-(1—>4)-)3- D-GlcNAc-(l-OMe). With the Ulex lectin, the thermodynamic parameters are A/r "-29, TAS -20.5 kcal mol" . Again a decrease in entropy is observed. However, with the Psophocarpus lectin, the figures are ATT "- 5.4, TA5° -I- 0.8 kcal mol" . In the latter case, the small increase in entropy seems to indicate an important hydrophobic effect. 

Ulex europaeus seeds contain two agglutinins an L-fucose-binding lectin (U. europaeus I, UFA I) and U. europaeus II (UEA II), which is most potently Inhibited by V-acetylated chitodextrins and L-Fuc(al-2)Gal( 3l-4)GlcNAc. 

Anti-L//ex europaeus 1 lectin (P) Dako 1 4000 NT Recognition of endothelial tumors via binding of Ulex europaeus 1 lectin... 

Ulex europaeus 1 (lectin) Dako 1 1000 NT Recognition of endothelial tumors... 

KER, keratin, detected by a mixture of GAMS.2, MAK-6, and AEl /AE3 EMA, epithelial membrane antigen VIM, vimentin DES, desmin MSA, muscle-specific actin SMA, smooth muscle (alpha isoform) actin GALD, h-caldesmon S-IOOP, S-100 protein OCN, osteocalcin LM, laminin UL, Ulex europaeus I lectin binding FS, fibrosarcoma SGRMS, spindle cell rhabdomyosarcoma LMS, leiomyosarcoma MPNST, malignant peripheral nerve sheath tumor MSS, monophasic spindle cell synovial sarcoma SCAS, spindle cell angiosarcoma KS, Kaposi s sarcoma FOS, fibroblastic osteosarcoma. 

Ordonez NG, Batsakis JG. Comparison of Ulex europaeus I lectin and factor Vlll-related antigen in vascular lesions. Arch Pathol Lab Med. 1984 108 129-132. 

Yonezawa S, Maruyama I, Sakae K, et al. Thrombomodulin as a marker for vascular tumors Comparative study with factor VIII and Ulex europaeus I lectin. Am J Clin Pathol. 1987 88 405-411. 

Holthofer H, Virtanen I, Kariniemi AL, et al. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab Invest. 1982 47 60-66. 

Heng MC, Fallon-Friendlander S, Bennett R. Expression of Ulex europaeus agglutinin I lectin-binding sites in squamous cell carcinomas and their absence in basal cell carcinomas. Indicator of tumor type and differentiation. Am J Dermatopathol. 1992 14 216-219. 

CDCs are positive for EMA, vimentin, and the lectin Ulex europaeus Agglutinin (UEA-1). The diagnosis of CDC carries a poor prognosis with the majority of patients dying of metastatic disease within 2 years of... 

For example, glomerular microvascular endothelial cells in culture should maintain the characteristic fenestration, the presence of Weibel-Palade bodies (both observed by transmission electron microscopy), the basal expression of von Willebrand factor and CD 31 (plate-let-endothelial cell adhesion molecule-1) and the binding of lectin Ulex Europaeus (for human cells) [18,19]. 

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