Ulex lectins

Ulex lectin UEAI Ulex europaeus a-I.-Fucose... 

Complex, blood-group-active substances have been employed as inhibitors of hemagglutination.196,693 Osawa and Matsumoto found692 inhibition of both Ulex lectins by human A-, B-, and H-active substances, whereas Lea substance and porcine, submaxillary mucin bound only to Ulex II. Chuba and coworkers693 reported that substances from porcine stomach (A-active), equine stomach (B-active), and baboon stomach (A-, B-, and O-active substances), as well as human A-, B-, and O-active substances, bound equally well to both U. europeus lectins. However, porcine, submaxillary mucin and nonsecretor saliva inhibited only Ulex II activity. 

Other associations have been investigated by the same methods. Lectin UE-I from Ulex europaeus (Section 15.4.3) and PT-II from Psophocarpus tetragonolobus both bind the H-type trisaccharide glycoside a-L-Fuc-(l—>2)-j3-D-Gal-(1—>4)-)3- D-GlcNAc-(l-OMe). With the Ulex lectin, the thermodynamic parameters are A/r "-29, TAS -20.5 kcal mol" . Again a decrease in entropy is observed. However, with the Psophocarpus lectin, the figures are ATT "- 5.4, TA5° -I- 0.8 kcal mol" . In the latter case, the small increase in entropy seems to indicate an important hydrophobic effect. 

CD31 (54), Factor Vlll-related antigen (55), and CD34 (56) cannot often be demonstrated in poorly differentiated vascular tumors. The use of the lectin Ulex europaeus agglutinin 1 (UEA-1) (57) is a more sensitive method to detect endothelial cells. Like other lectins, however, UFA-1 reacts with many other cell types. 

Figure 4. Pea stem material was sequentially extracted with hot 70% ethanol, 0.1 M EDTA and 4% KOH-0.1% NaBH4 to leave xyloglucan-cellulose cell wall ghosts . Binding of fluorescent fucose-binding lectin from Ulex europeus as visualized by fluorescence microscopy shows xyloglucan distributed over the whole wall surface. Photograph courtesy of Dr. T. Hayashi.

Cell division is stimulated by lectins (for a review, see Ref. 188) and by other saccharide-directed reagents, for example, sodium periodate and D-galactose oxidase after desialylation,189 but there is no evidence that it is the fucopyranosyl residues that are the specific receptors in these instances. The L-fucose-specific lectins from, for example, Lotus tetragonolobus and Ulex europeus are not mitogenic. 

Cuatrecasas (45). The Dolichos biflorus lectin was a gift from Dr. Marilynn Etzler (46). Ulex europeus and Bandeirea simplicifolia lectins were purified in our laboratory and iodinated with Nal25i (carrier-free) by Sepharose 4B-bound lactoperoxidase (47)and diazotized iodoaniline coupling (48) procedures, respectively. 

Ulex Europeus [ I]Lectin Binding to Neuroblastoma Cell Surfaces... 

The specific activity of Ulex europeus [125I]lectin was 0. 5 x 10 cpm per mg of... 

It is well known that intestinal M cells have the ability to efficiently transcytose macromolecules and inert particles, which has stimulated researchers to target M cells for oral delivery of macromolecular drugs and particles.26,52 For example, Ulex europaeus 1 (UEAl), a lectin specific for a-L-fucose residues, selectively binds to mouse Peyer s patch M cells.56 It has become apparent that UEAl can be used to target macromolecules to mouse Peyer s patch M cells and to enhance macromolecular... 

Clark, M. A., Jepson, M. A., Simmons, N. L, and Hirst, B. H. Selective binding and transcytosis of Ulex europaeus 1 lectin by mouse Peyer s patch M-cells in vivo. Cell Tissue Res. 282 455-461, 1995. 

Synthesis of Type 2 Human Blood Group Antigenic Determinants. The H, X and Y Haptens and Variations of the H Type 2 Determinants as Probes for the Combining Site of the Lectin 1 of Ulex europaeus, O. Hindsgaul, T. Norberg, J. LePendu, and R. U. Lemieux, Carbohydr. Res., 109 (1982) 109-142. 

Molecular Recognition XI. The Synthesis of Extensively Deoxygenated Derivatives of the H-type 2 Human Blood Group Determinant and their Binding by an Anti-H-type 2 Monoclonal Antibody and the Lectin 1 of Ulex europaeus, U. Spohr, E. Paszkiewicz-Hnatiw, N. Morishima, and R. U. Lemieux, Can. J. Chem., 70 (1992) 254-271. 

The recognition of three different epitopes for the H-type 2 human blood group determinant by lectins of Ulex europaeus, Galactia... 

There are two lectins of distinct, sugar-binding specificity in Ulex extracts.196,225 Using ethanol fractionation, Flory225 initially discriminated between Ulex I, which is readily inhibited by L-fucose derivatives, and Ulex II, which binds /3-D-glucosides. (A comprehensive discussion of Ulex II purification and properties is presented in Section III,5.) Flory s 50-70% ethanol precipitate enriched in Ulex I, was slightly inhibited by D-arabinose, D-ribose, and D-lyxose, in addition to L-fucose.225 This preparation agglutinated human, epithelial, cheek-cells of secretory individuals only.225... 

Fig. 15.—Human IgA, Glycopeptide871 Showing the Carbohydrate-binding Loci for Various Lectins, [a. Limulus polyphemus b, Triticum vulgaris c, Ricinus communis d, Sophora japonica e, Abrus precatorius f, Cytisus sessilifolius g, Phaseolus vulgaris-, h, Canavalia ensiformis i, Lens culinaris j, Pisum sativus k, Vicia faba-, 1, Bandeiraea simplicifolia II m, Solanum tuberosum and n, Ulex europeus II.]...

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