Oligosaccharides specificity

Velupillai, P. and Ham, D.A. (1994) Oligosaccharide-specific induction of interleukin-10 production by B220+ cells from schistosome-infected mice - a mechanism for regulation of CD4+ T-cell subsets. Proceedings of the National Academy of Sciences USA 91, 18—22. 

Kasai, K Oligosaccharide specificity of galectins a search by frontal affinity chromatography. Biochim Biophys Acta 2002, 1572, 232-254. Hirabayashi, J., Arata, Y, Kasai, K. Glycome project concept, strategy and preliminary application to Caenorhabditis elegans. Proteomics 2001, 1, 295-303. 

Bolmstedt, A.J., et al. 2001. Cyanovirin-N defines a new class of antiviral agent targeting N-linked, high-mannose glycans in an oligosaccharide-specific manner. Mol Pharmacol 59 949. 

Fig. 1 Cell membrane of a gram-negative bacteria, including the lipopolysaccharide, protein, and phospholipid of the outer membrane (O-specific = oligosaccharide-specific).

H. Leffler, U.J. Nilsson, Efficient and expedient two-step pyranose-retaining fluorescein conjugation of complex reducing oligosaccharides galectin oligosaccharide specificity studies in a fluorescence polarization assay, Bioconjugate Chem. 2003, 14, 1289-1297. 

A combination between MALDI, TLC, and antibody detection was also recently suggested [49] whereby the authors used the following workflow (1) TLC separation of putative cancer-associated GSLs from human hepatocellular and pancreatic tumors, (2) their detection with oligosaccharide-specific proteins, and (3) the in situ MS analysis of the previously protein-(antibody)-detected GSL. Detection limits of less than 1 ng of immunostained GSL could be obtained at these conditions. It is a particular advantage of this approach that only crude lipid extracts of biological sources are required for TLC/IR-M ALDI/MS and no laborious previous GSL purification is needed. 

Velupillai, P and Ham, DA (1994) Oligosaccharide-specific induction in interleukin 10 production by B22+ cells from schistosome-infected mice A mechanism for regulation of CD4-I-T-cell subsets. Proc Natl Acad Sci U S A91, 18-22. 

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