Syngeneic models

Demeuse P, Kerkhofs A, Struys-Ponsar C, Knoops B, Remade C, van den Bosch de Aguilar P. Compartmentalized coculture of rat brain endothelial cells and astrocytes a syngenic model to study the blood-brain barrier. J Neurosci Methods 2002 121 21-31. 

Bruckheimer, E., Harvie, R, Orthel, J., et al. 2004. In vivo efficacy of folate-targeted hpid-protamine-DNA (LPD-PEG-Folate) eomplexes in an immunocompetent syngeneic model for hreast adenocarcinoma. Cancer Gene Then 11 128-34. 

CT-2103 has been studied in a wide variety of S5mgeneic and xenogeneic tumour models (Tables 3A and 3B). In aH cases it has enhanced efficacy when its maximum tolerated dose (MID) was compared to that of free paclitaxel administered in Cremophor EL/ethanol. In the mouse strains that were evaluated, the single dose IV or IP MTD for CT-2103 was approximately twice that of paclitaxel administered in Cremophor EL/ethanol [80 mg/kg for paclitaxel in immunocompetent mice (syngeneic models) and 60-70 mg/kg in nu/nu mice respectively compared with 160-200 mg/kg in syngeneic mice and 120-150 mg/kg in nu/nu mice for CT-210311.18 example of the enhanced efficacy of CT-2103 in nude mice bearing the HT-29 human colon cancer is shown in Eigure 6. 

Host resistance Bacterial models—Listeria monocytogenes (mortality or spleen clearance) Streptococcus species (mortality) Viral models—influenza (mortality) Parasitic models—Plasmodium yoelii (parasitemia) or Trichinella spiralis (muscle larvae counts and worm expulsion) Syngeneic tumor models—PYB6 sarcoma (tumor incidence) B16F10 melanoma (lung burden). 

The primary clinical application of the antiproliferative effects of the bisindole alkaloids has been in the treatment of human neoplasms. A variety of experimental antitumor systems have been used to study the antitumor properties of these alkaloids in animal models, and much of the information presented for specific compounds in this chapter highlights these effects. Early studies employed syngeneic mice bearing hematopoietic... 

Depletion of JARIDIB inhibits proliferation of breast cancer cell lines and tumor growth in a syngeneic mouse mammary tumor model. 

We tested the effect of LPS in a model of peritoneal carcinomatosis (solid tumor) induced by PROb colon cancer cells in syngeneic BDEX rats. We showed that i.p. injections of LPS from E. coli can cure 20 % of the rats [157], Comparing the effect of LPS from different strains in this model, we found that the efficacy depends on the bacterial strain and on the structure of the lipid A used. Whatever the lipid A used, we have shown a correlation with in vitro macrophage secretion of IL-ip but not with NO, TNF-a or IL-6 [83],... 

When crude endotoxin from the heptose-less mutant of Salmonella typhimurium is combined with trehalose dimycolate from mycobacteria in oil droplets and injected directly into established tumors (line 10 hepatocellular carcinoma) in syngeneic guinea pigs, rapid regression of the tumors occurs and over 90% of the animals are cured. The three required components for activity in this tumor model are (a) the endotoxin (b) the mycobacterial adjuvant, trehalose dimycolate and (c) a compound satisfying the minimal structural requirement (muramyl dipeptide) for adjuvant activity by bacterial cell wall materials. The mycobacterial cell wall skeleton is able to replace the latter two components. 

Perhaps the most common model used to assess outcomes uses syngeneic mice given a subcutaneous injection of a transplantable, syngeneic tumor or xenogenic tumor injected into nude or SCID mice. These tumor models allow a broad range of outcome strategies in response to therapeutic intervention. Alternatively, models... 

Peer, D., and Margalit, R. (2004), Tumor-targeted hyaluronan nanoliposomes increase the antitumor activity of liposomal doxorubicin in syngeneic and human xenograft mouse tumor models, Neoplasia, 6, 343-353. 

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