Mouse xenograft tumor model

The hits identified from these screens also displayed interesting structure-activity relationships. Almost all of the top performers had a hydrophobic diacrylate monomer, and among the most effective polymers amino dialcohol and bis(secondary amines) monomers were well represented. In vivo evaluation of the best-performing polymer, labeled C32, was compared to PEI for toxicity and DNA delivery in a mouse xenograft tumor model with human prostate cancer cells. C32 transfected a luciferase DNA plasmid fourfold better than PEI and 26-fold more efficiently than the naked... 

Pimm, M. V., Durrant, L. G., and Baldwin, R. W. Influence of circulating antigen on the biodistribution and tumor localization of radiolabelled monoclonal antibody in a human tumor Nude mouse xenograft model. Eur. J. Cancer. Clin. Oncol. 25 1325-1332, 1989. 

No rodent bioassay Tumor promotion potential was evaluated by first identifying human tumor cell lines that bind natalizumab in vitro Tumor promotion potential was then evaluated by assessing tumor growth in athymic nude mice after tumor transplantation (sc) followed by treatment with natializumab. Natalizumab did not show any tumor promoting potential on two oc4 expression tumors (leukemia, melanoma) in a nude mouse xenograft model Not genotoxic (Ames, in vitro chromosomal aberrations in human PBLs)... 

Peer, D., and Margalit, R. (2004), Tumor-targeted hyaluronan nanoliposomes increase the antitumor activity of liposomal doxorubicin in syngeneic and human xenograft mouse tumor models, Neoplasia, 6, 343-353. 

This comparative ABPP analysis was subsequently extended to a more sophisticated in vivo model of human cancer-breast cancer xenografts grown in immunodeficient mice [46]. The mixed species nature of the xenograft model enabled the discrimination of active enzymes that were tumor-associated (human) or host-derived (mouse), resulting in the identification of several different classes of activities, including carcinoma enzyme activities expressed selectively in culture or in xenograft tumors, as well as host stromal activities that either infiltrated or were excluded from xenograft tumors. 

T cells in vitro [121]. The Tat-p27 protein dose-dependently induced cell cycle arrest at Gl. Snyder et al. demonstrated that the Tat-p27 tumor suppressor protein actually inhibited tumor growth in two mouse models, such as a HI299 subcutaneous solid tumor xenograft model and a more clinical-relevant peritoneal tumor model [122]. 

Fig. 8 Antitumor activity of aptamer-siRNA chimera in a mouse xenograft model of prostate cancer, (a) Chimeric siRNA and PBS were administered intratumorally in mice bearing either PSMA-negative prostate cancer cells, PC-3 left panel) or PSMA-positive cells, LNCaP right panel). Tumors were measured every 3 days and analyzed using a one-way ANOVA ( P<0.0001 P<0.001 P<0.01, n= 6-8). (b) Histology of LNCaP tumors treated with chimeric RNAs. Formalin-fixed tumors were embedded in paraffin and stained with hematoxylin and eosin. (Adopted from Nature Biotechnology 2006 24 1005-1015)...

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