Proteins lung surfactant

The well-defined helical structure associated with appropriately substituted peptoid oligomers (Section 1.6) can be employed to fashion compounds that closely mimic the stracture and function of certain bioactive peptides. There are many examples of small helical peptides (<100 residues) whose mimicry by non-natural ohgomers could potentially yield valuable therapeutic and bioactive compounds. This section describes peptoids that have been rationaUy designed as mimics of antibacterial peptides, lung surfactant proteins, and coUagen proteins. Mimics of HIV-Tat protein, although relevant to this discussion, were described previously in this chapter (Sections 1.3.2 and 1.4.1). 

Peptoid-Based Mimics of Lung Surfactant Proteins... 

Lipp, M.M., Lee, K.Y.C., Waring, A., and Zasadzinski, J.A. Fluorescence, polarized fluorescence, and Brewster angle microscopy of palmitic acid and lung surfactant protein B monolayers. Biophys. J. 1997, 72, 2783-2804. 

Seurynck, S.L., Johnson, M., and Barron, A. E. Simple helical peptoid analogues of lung surfactant protein B. 2003. (Manuscript in preparation). 

Guillot, L., et al., Cutting edge The immunostimulatory activity of the lung surfactant protein-A involves Toll-like receptor 4, J. Immunol. 168, 12, 5989, 2002. 

Oosterlaken-Dijksterhuis MA, Haagsman HP, van Golde LM, et al. Characterization of lipid insertion into monomolecular layers mediated by lung surfactant proteins SP-B and SP-C. Biochemistry 1991 30(45) 10965-10971. 

Cal Bio cloned the gene that encodes human lung surfactant protein, a major step toward reducing a premature birth complication. 

Lausen, M., Lynch, N., Schlosser, A., Tornoe, I., Sackmose, S. G., Teisner, B., Willis, A. C., Crouch, E., Schwaeble, W., and Holmskov, U. (1999). Microfibril-associated protein 4 is present in lung washings and binds to the collagen region of lung surfactant protein D. / Biol. Chem. 274, 32234-32240. 

Pulmonary alveolar proteinosis Lung surfactant protein C Local... 

Lung surfactant protein C Res 9-22 (WWVVVLVVVVIV) (deMeUo and Lin 2001 Hosia et al. 2002 Schmitt-Bemard et al. 2000 Seymour and PresneiU 2002 Trapnell et al. 2003) Pulmonary alveolar proteinosis... 

Section six is devoted to Amphiphiles at Vapor-Liquid Interfaces. Zasadzinski and coworkers present a review of lung surfactant protein and the effects of such protein on altering phase structures in palmitic acid monolayers. They articulate some... 

Effects of curcumin, an antioxidant lipophilic drug on membrane structure by Solid-State NMR Spectroscopy. xviii. The elfect of the cationic, helical peptide based on the essential lung surfactant protein B on oriented lipid bilayers characterized by H... 

Laboratory aberrations are nonspecific (2). The erythrocyte sedimentation rate (ESR) is elevated in >60% of patients circulating antinuclear antibodies (ANA) or rheumatoid factor are present in 10% to 26% (2,15). These serological parameters do not correlate with the extent or activity of the disease and have no prognostic value (2,15). Elevations of the glycoprotein KL-6 (19,20) and the lung surfactant proteins (SP) A and D (21) have been noted in serum and bronchoalveolar lavage fluid (BALF) in patients with IPF, and may have prognostic value. These assays are available in only a few research laboratories and additional studies are required to assess their specificity and clinical role. 

Honda Y, Kuroki Y, Shijubo N, et al. Aberrant appearance of lung surfactant protein A in sera of patients with idiopathic pulmonary fibrosis and its clinical significance. Respiration 1995 62(2) 64-69. 

Breathing is enabled by lung surfactant, a mixture of proteins and lipids that forms a surface-active layer and reduces surface tension at the air-water interface in lungs. Surfactant protein B (SP-B) is an essential component of lung surfactant. Researchers probed the mechanism underlying the important functional contributions made by the N-terminal 7-residues of SP-B, a region sometimes called the insertion sequence . These studies employed a construct of SP-B, SP-B (1-25,63-78), also called Super Mini-B, which is a 41-residue peptide with internal disulfide bonds comprising the N-terminal 7-residue insertion sequence and the N- and C-terminal helixes of SP-B. CD, solution NMR, and SS NMR were used to study the structure of SP-B (1-25,63-78) and its interactions with phospholipid bilayers. Comparison of results for SP-B (8-25,63-78) and SP-B (1-25,63-78) demonstrates that the presence of the 7-residue insertion sequence induces substantial disorder near the center of the lipid bilayer. ... 

Wang JY, Kishore U, Lim BE, Strong P, Reid KB. Interaction of human lung surfactant proteins A and D with mite Dermatophagoides pteronyssinus) allergens. Clin Exp Immunol 1996 106 367-373. 

Malhotra R, Haurum J, Thiel S, Sim R. Interaction of C1 q receptor with lung surfactant protein A. Eur J Immunol 1992 22 1437-1445. 

---