Reaction Rate and Substrate Concentration

Figure 3.5 Relation between reaction rate and substrate concentration.

Parameters for the simple model were determined graphically by Eadie-Hofstee plotting of initial reaction rates and substrate concentrations. Details are given elsewhere (30). As has been observed in hydrolysis of other solid substrates, a residue of non-lysed substrate was found at extended reaction times, when dY/dt tended toward zero. The extent of reaction was strongly dependent on initial substrate and enzyme concentrations (33,34). An empirical funciton for Y was fitted to the ultimate turbidity data for lysis runs at a variety of initial yeast and enzyme concentrations using a least squares method. The calculated values for Yco were used in the simulations (30). Figure 3 shows results of the simple model. 

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