Substrate and Product Concentration in Enzymes Following Classical Michaelis-Menten Kinetics

Modulation by Substrate and Product Concentration in Enzymes Following Classical Michaelis-Menten Kinetics 

Theoretically, any metabolic pathway could be regulated by the availability of substrate. A reduction of substrate concentration will decrease the activity of an enzyme, and this could result in a decreased flux stepwise through the entire pathway. However, studies demonstrating the relative constancy of substrate levels in vivo imply that such a mechanism, while perhaps important for single enzymes, is not operative for pathways in higher animals. Nonetheless, the plasma fatty 

Product can also affect enzyme activity. If the product of an enzyme-catalyzed reaction is not removed, thermodynamic inhibition will be encountered as the reaction approaches equilibrium and the 

While product inhibition in vitro is a common observation, there appear to be few examples which have physiologic meaning. Recently, Richman and Meister (1975) demonstrated the inhibition of glutamyl- 

Modulation of Enzyme Activity by Binding of Small Molecules to Regulatory Sites 

---