Double Monod kinetics

Figure 5.25. Representation of the influence of endogenous metabolism (as per Equ. 5.76 with kf) on obtaining model parameters for microbial growth with Monod kinetics using a double reciprocal plot. (From Moser and Steiner, 1975.)...

Figure 6.3. Linear plot for Monod kinetics according to Equ. 6.15 in the form of a double reciprocal plot (cf. Fig. 4.24c) applied to a CSTR with D = 1IT= fi, which is suitable for parameter estimation ( max and Ks).

Monod kinetics with double substrate limitation (cf. Sect. 5.5) ... 

Lineweaver-Burk plot A double-reciprocal plot used to determine the two constants featured in simple enzyme kinetic equations such as Michaelis-Menten kinetics, Monod kinetics, and in similar adsorption isotherm models such as the Langmuir adsorption isotherm. The constants are determined from the intercept with the y-axis and the gradient (see Fig. 26). It was devised and published in 1934 by American chemist Hans Lineweaver (1907-2009) and American biochemist Dean Burk (1904-1988). 

A double reciprocal plot of kinetic data for a biochemical reaction that obeys a Monod rate law will yield a value of Pmax from the reciprocal of the intercept of the y-axis. The corresponding slope is equal to At high val-... 

This is in agreement with the requirements proposed by Sykes (1973) and defines the shaded region in Fig. 5.51, indicating that both double-S limitations and a switch between limiting substrates are quite common. However, Equ. 5.172 holds only for kinetic models such as the Monod and exponential models approaching saturation asymptotically. To handle this problem, it is desirable to overlay curves of constant jll on Fig. 5.51 for some of the models discussed earlier. Two different philosophies can be distinguished interactive and noninteractive models. 

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See also in sourсe #XX -- [ ]

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