Soy protein concentrates

Commercial soy protein concentrates typically contain 70 to 72% cmde protein, ie, nitrogen x 6.25, dry wt basis. Soy protein isolates are prepared from desolventhed, defatted flakes. A three-stage aqueous countercurrent extraction at pH 8.5 is used to disperse proteins and dissolve water-soluble constituents. Centrifugation then removes the extracted flakes, and the protein is precipitated from the aqueous phase by acidifying with HCl at pH 4.5. 

Pish protein concentrate and soy protein concentrate have been used to prepare a low phenylalanine, high tyrosine peptide for use with phenylketonuria patients (150). The process includes pepsin hydrolysis at pH 1.5 ptonase hydrolysis at pH 6.5 to Hberate aromatic amino acids gel filtration on Sephadex G-15 to remove aromatic amino acids incubation with papain and ethyl esters of L-tyrosine and L-tryptophan, ie, plastein synthesis and ultrafiltration (qv). The plastein has a bland taste and odor and does not contain free amino acids. Yields of 69.3 and 60.9% from PPG and soy protein concentrate, respectively, have been attained. 

Protein-Based Substitutes. Several plant and animal-based proteins have been used in processed meat products to increase yields, reduce reformulation costs, enhance specific functional properties, and decrease fat content. Examples of these protein additives are wheat flour, wheat gluten, soy flour, soy protein concentrate, soy protein isolate, textured soy protein, cottonseed flour, oat flour, com germ meal, nonfat dry milk, caseinates, whey proteins, surimi, blood plasma, and egg proteins. Most of these protein ingredients can be included in cooked sausages with a maximum level allowed up to 3.5% of the formulation, except soy protein isolate and caseinates are restricted to 2% (44). 

Table 6. Analyses of Commercial Soy Protein Concentrates and Isolates ...

In order to produce soy protein, soybeans are first dehulled, flaked, and defatted to make white flakes . Soy protein concentrates are obtained by removing a portion of the carbohydrates from defatted and dehulled soybeans. Alcohol extraction is the method most commonly used to manufacture soy protein concentrates even though it results in the loss of isoflavones. Soy protein concentrates retain most of the fiber in the original soybean and must contain at least 65% protein on a moisture-free basis to meet quality standards. The most concentrated source of soy protein is soy protein isolates (or isolated soy protein, ISP), which is required to be at least 90% protein on a moisture-free basis. It is heat-treated during processing to insure inactivation of trypsin inhibitors. Most isolated soy protein is manufactured by water extraction from defatted and dehulled soybeans and it retains the natural isoflavones. 

POTTER s M, FERTILE J and BERBER-JIMENEZ M D (1996) Soy protein concentrate and isolated soy protein similarly lower blood serum cholesterol hut differently affect thyroid hormones in hamsters. J Nutr. 126 (8) 2007-11. 

Soya Proteins. Early attempts to make albumen substitutes from soya protein also ran into problems. A bean flavour tended to appear in the finished product. A solution to these problems has been found. Whipping agents based on enzyme modified soy proteins are now available. The advantage of enzymatic modification is that by appropriate choice of enzymes the protein can be modified in a very controlled way. Chemical treatment would be far less specific. In making these materials the manufacturer has control of the substrate and the enzyme, allowing the final product to be almost made to order. The substrates used are oil-free soy flakes or flour or soy protein concentrate or isolate. The enzymes to use are chosen from a combination of pepsin, papain, ficin, trypsin or bacterial proteases. The substrate will be treated with one or more enzymes under carefully controlled conditions. The finished product is then spray dried. 

Figure 2. Relationship between calcium solubility and pH after complete digestion for four soy products. Key solid line, full-fat soy flour long-dashed line, soy protein isolate short-dashed line, soy protein concentrate and dotted line, defatted soy flour...

Table I. Effects of 17o Orthophosphate, Tripolyphosphate, and Hexametaphosphate on Calcium Solubility from Ground Beef or Soy Protein Concentrate Subjected to In Vitro Gastric and Gastrointestinal Digestions...

Some of the uses of soy proteins to augment meat proteins are summarized in Table III. Textured soy flour, though not included in that table, can be used in the same meat systems, where soy grit or coarse soy protein concentrate are used. Besides the economics associated with the use of soy protein, they are utilized in such meat products because of their functional and nutritional properties. 

Meat system 50% Protein 70% Protein dry basis Soy protein concentrate 90% Protein dry basis Isolated soy protein... 

An evaluation of the composition of the cooking juices, as presented in Table IV, demonstrates, by difference, that fat is retained to a greater extent by the soy concentrate meat product than by the soy flour meat product or the ground beef. The meat product containing soy flour lost more fat during cooking than did the all-beef (Table IV). Similar results were reported by Anderson and Lind ( ). When soy protein concentrates are used in canned meat products like chili, the fat islands within the chopped meat products and the fat cap are eliminated (10). When 4% soy concentrate was added to a minced pork product, cook out of fat and moisture was reduced 31% for pasteurized product and 34% for sterilized product. 

In the study by Thompson, et al. (11), the ml of gel released per 100 g emulsion for the reference emuTsion without soy, with soy isolate (SIF), soy concentrate (SCF) or soy flour (SF) was 6.07, 5.83, 5.49 and 3.08, respectively, when the hydration ratios were 1 4 (flourrwater) for SIF, 1 3 for SCF and 1 2 for SF. The ml gel released per 100 g emulsion containing 10, 15, 20, and 25% soy protein was 6.70, 5.01, 3.94 and 3.57, respectively. When soy protein concentrate was incorporated into an emulsion at the 3.5% level, the processing yields, textural profile and sensory textural attributes of frankfurters were not different among the products with and without added soy concentrate (13). An objective measure of compression and shear modulus indicated that soy protein concentrate incorporated into frankfurters at the 3.5% level had no effect on batter strength or texture ( M). The addition of a cottonseed protein to frankfurters to replace 5, 10 or 15% of the meat resulted in higher pH, less cured color, less firmness of skin, softer texture and reduced desirability as judged by a sensory panel (J5J. 

Advances in soy protein processing technology have allowed extensive diversification of protein product applications. More sophisticated soy protein products now manufactured have more functionality, better performance, more consistency and better flavor than commercially available defatted soy flour and grits (50% protein dry basis). Among these products are improved textured soy flours, concentrates, and isolates (50%, 70% and 90% protein dry basis, respectfully), functional and non-functional soy protein concentrates (70% protein dry basis) and highly soluble, highly functional isolated soy proteins (90% protein dry basis) (6-8 14-18). 

Soy Protein Concentrates. Both non-functional (low or no solubility) and functional (good solubility, emulsification capacity, and dispersibility) soy protein concentrates (70% protein, dry basis) are commercially available for use in meat products (2-4, 6, j), 15) Normally, a highly functional product with no harsh or bitter flavors is desirable. When used to replace lean meat, non-hydrated concentrate can be used at levels up to 6-7% in finished nonspecific emulsion meats Higher replacement levels or formulas with specific cost/nutrition requirements may use soy protein concentrate with a judicious amount of textured soy protein (6). Excellent yields, cost savings, texture, flavor and nutrient profiles are possible. However, most soy protein concentrates lack sufficient solubility or sufficiently low viscosities to be used in brines for absorption or injection into whole muscle tissue. When legal standards for protein content exist (13), more concentrate must be used to achieve legal minimums. Brine viscosities increase and uniform distribution of brine components throughout the specific whole muscle piece is restricted. Finished product appearance and flavor are easily compromised. Thus, use of soy protein concentrates in whole muscle applications is limited. 

Eight men fed DSM or soy protein concentrate diets, each for 10-day periods 72mg ascobric acid consumed with each meal... 

Eight subjects fed (10 days) formula diets with either (a) DSM or (b) soy protein concentrate as protein source extrinsic zinc label (fecal monitoring method)... 

Plant protein Wheat gluten Peanut protein Soy protein concentrate Promine-R... 

Figure 2. Water binding (grams of unfrozen water/gram of solids) by soy protein preparations, each containing 1 g of water/g of solids, as a function of protein content, where % protein (on a solids basis) = % N X 6.25 (O) soy protein isolate B (9) soy protein concentrate (/ ) soy flour (defatted) fCJl carbohydrate-enriched fraction of soy concentrate f32j.

Wang and Kinsella (19) studied the fat absorption, and other properties of alfalfa leaf protein (ALP) concentrate and used the soy protein concentrate and isolate Promosoy-lOO and Promine-D,respectively, as the references. The fat absorption values are reported in ml oil/g sample. Converting these to percent fat absorbed (based on the specific gravity of peanut oil) results in values that are higher than those reported by Lin et al. (17) this was most evident in the case of the isolate. 

Sato et a2- (34) demonstrated that a variety of common meat additives, inclucnrTg cottonseed flour, nonfat dry milk, spray-dried whey, wheat germ, and textured soy flour, inhibited WOF in the meat system. These products may have exerted their inhibitory effect on WOF through the Maillard reaction, since most of them contain some reducing sugars. Pratt (40) reported soybeans and soy protein concentrate had an inhibitory effect upon development of WOF and was able to demonstrate that the active components are water soluble. Fractionation and analysis of the water-soluble fraction showed the antioxidant activity was due to the presence of isoflavones and hydroxylated cinnamic acids (40). This confirms earlier work showing that the flavonoTcis present in plant extracts inhibit oxidation in sliced roast beef (41 ). 

Ingredients. All ingredients were obtained commercially along with compositional information and consisted of corn starch (National Starch, Bridgewater, NJ), whey protein concentrate (WPC) and sodium caseinate (SC) (Leprino Foods, Denver, CO), defatted soy flour (DSF) (Archer Daniels Midland, Decatur, IL), soy protein concentrate (SPC) (Central Soya Company, Fort Wayne, IN), and gluten (G) (Ogilvie Mills Ltd., Montreal, Canada). 

Development of serum-free medium has great value for large-scale biopesticide production. The latest formulations are serum-free, such as SF900II (GIBCO /Invitrogen) and EX-CELL (JRH Biosciences). Cell culture medium supplementation using yeast extract (usually from alcoholic fermentation processes), milk, or soy protein concentrates, can also be an alternative to decrease cell culture medium costs (more details can be found in Chapter 5). 

Identification Soy Protein Concentrate exhibits the compositional profile specified below with respect to Ash, Fat, Loss on Drying, and Protein. 

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