Sodium ethylenediaminetetraacetate EDTA

If the analyte is very volatile (e.g., propane or butane) and a quantitative analysis is required, a blood sample should be collected directly into the headspace vial in which the analysis will be carried out. Many other volatile compounds are relatively stable in blood and other tissues if simple precautions are taken. In the case of blood, the container used for the sample should be glass, preferably with a cap lined with metal foil greater losses may occur if plastic containers are used. The tube should be as full as possible and should only be opened when required for analysis and then only when cold (4°C). If the sample volume is limited, it is advisable to select the container to match the volume of blood so that there is minimal headspace. An anticoagulant [sodium ethylenediaminetetraacetate (EDTA) or lithium heparin] should be used. Specimen storage between - 5 and 4°C is recommended and 1% (w/v)... 

Ethylene cyanohydrin, 1 358, 404 10 639 Ethylenediamine, 2 129 8 485 as chelant, 5 709 molecular formula, 5 712t physical properties, 8 486t pK values, 8 487t prices of commercial, 8 496t sodium solubility in, 22 764 typical specifications, 8 496t Ethylenediaminetetraacetate (EDTA), 7 576... 

Salt and chelate formation with edetate (ethylenediaminetetraacetate, EDTA). A In a solution of calcium disodium salt of EDTA, the sodium and hydrogen ions are chemically and biologically available. B In solutions of calcium disodium edetate, calcium is bound by coordinate-covalent bonds with nitrogens as well as by the usual ionic bonds. C In the lead-edetate chelate, lead is incorporated into five heterocyclic rings. 

On Day 2, sub-samples of the effluent are adjusted to pH 3 and 11 (pH 9 for the Cl 8 treatment), and then filtered, aerated or passed through a Cl 8 column. After the manipulations are done, the samples are adjusted back to the initial pH of the effluent (pH / ) and tested for toxicity. The Ethylenediaminetetraacetate (EDTA), sodium thiosulfate and graduated pH adjustments are also conducted on Day 2, but testing is delayed until Day 3. The delay allows EDTA to complex with metals, thiosulfate to react with oxidative substances and metals, and pH to stabilize in the graduated pH test. 

N EDTA/0.2 N TRIS Solution Transfer 18.6 g of disodium ethylenediaminetetraacetate (EDTA) and 6.05 g of tris (hydroxymethyl)aminomethane (TRIS), both accurately weighed, into a 250-mL beaker. Add 200 mL of hot, deionized water, and stir until dissolved. Adjust the pH to 7.5 to 7.6 by adding 5 N sodium hydroxide. Cool the solution, and adjust the pH to 8.0 with 5 N sodium hydroxide. Transfer the solution into a 250-mL volumetric flask, and dilute to volume with deionized water. Mix well, and store in a plastic container. 

Some strains of P. aeruginosa are resistant to BAC and, in fact, can be grown in solutions concentrated in this agent. This has caused great concern because of the virulent nature of this organism in ocular infections, as discussed previously. Thus, it was an important finding in 1958 that the acquired resistance could be eliminated by the presence of ethylenediaminetetraacetic acid (sodium edetate EDTA) in the formulation. This action of EDTA has been correlated with its ability to chelate divalent cations and is commonly used as a preservative aid (155). The use of EDTA, where it is compatible, is recommended in concentrations up to 0.1%. 

Fig. 12.3. The phosphorus composition of broiler litter from a farm in Delaware, USA, determined by extraction with a solution containing sodium hydroxide and ethylenediaminetetraacetate (EDTA) and solution nuclear magnetic resonance spectroscopy. The extract contained 97% of the total phosphorus in the litter, of which 40% was phosphate and 60% was myo-inositol hexakisphosphate (phytic acid). Traces of pyrophosphate were also detected. The inset spectrum shows the four signals from myo-inositol hexakisphosphate, corresponding to the positions of the phosphates on the inositol ring (C-1 to C-6). Details of analytical methodology can be found in Turner (2004).

Ethylenediaminetetraacetic acid or its sodium salt (EDTA) is a well-known com-plexing agent which can form an abundant variety of water-soluble complexes with all metals. EDTA is widely used in industry and environmental studies are concerned with the degradation of its complexes [2] or the bioaccumulation of platinum group metals [3]. Directly related to the aim of this book is the catalytic activity in various carbonylation reactions of the precursor [Ru(EDTA)(H20)], as reported by Taqui Khan and co-workers [4], In fact the two nitrogen and three carboxylate functions bind the ruthenium(III) center, and under a CO atmosphere this complex is reduced to the ruthenium(II) species [Ru(EDTA)(CO)] in which only one carboxylate group... 

SODIUM IRON(III) ETHYLENEDIAMINETETRAACETIC ACID (SODIUM IRON EDTA) (addendum)... 

Sodium iron(lll) ethylenediaminetetraacetic acid (sodium iron EDTA)... 

Ethylenediaminetetraacetic acid, ferric-sodium salt Ethylenediaminetetraacetic acid, sodium ferric salt. See Sodium ferric EDTA Ethylenediaminetetraacetic acid, sodium salt Ethylenediaminetetraacetic acid, tetrasodium salt. See Tetrasodium EDTA Ethylenediaminetetraacetic acid, tripotassium salt. See Tripotassium EDTA Ethylenediaminetetraacetic acid, trisodium salt. See Trisodium EDTA... 

Sodium ethylenediaminetetraacetate. See Tetrasodium EDTA Sodium 2-ethyl hexanoate CAS 19766-89-3 EINECS/ELINCS 243-283-8 Synonyms 2-Ethylcaproic acid sodium salt 2-Ethylhexanoic acid sodium salt Hexanoic acid, 2-ethyl-, sodium salt Sodium barbitol Sodium 2-EHT... 

Coat plates with a mixture of silica gel G (after Stahl) and sodium ethylenediaminetetraacetate (sodium EDTA). Mix 30 silica gel in a soln. of 9 g sodium EDTA in 50 ml water and coat to a thickness of 0.25 mm. Dry at 100 C for 30 min Solvent... 

Synonyms Acetic acid, (ethylenedinitrilo) tetra-, tetrasodium salt Edathanil tetrasodium Edetate sodium Edetic acid tetrasodium salt EDTA Na4 EDTA sodium salt EDTA tetrasodium salt N,N -1,2-Ethanediylbis (N-(carboxymethyl)) glycine tetrasodium salt Ethylenebis (iminodiacetic acid) tetrasodium salt N,N -Ethylene-diaminediacetic acid tetrasodium salt Ethylenediaminetetraacetic acid, sodium salt Ethylenediaminetetraacetic acid, tetrasodium salt Sodium ethylenediamine-tetraacetate Tetrasodium edetate Tetrasodium ethylenediamine tetraacetate Tetrasodium (ethylenedinitrilo) tetraacetate ClassiTication Substituted amine Definition Powdered sodium salt that reacts with metals Empirical C, jH,2N208 4Na Formula (NaOOCCH2)2NCH2CH2N(CH2COONa)2 Properties Wh. amorphous powd. freely sol. in water m.w. 380.20 dens. 6.9 lb/ gal m.p. > 300 C... 

EDTA (ethylenediaminetetraacetic acid, [60-00-4]) chelates any trace metals that would otherwise decompose the hydrogen peroxide [7722-84-1]. The amine is preheated to 55—65°C and the hydrogen peroxide is added over one hour with agitation the temperature is maintained between 60 —70°C. The reaction is exothermic and cooling must be appHed to maintain the temperature below 70°C. After all the peroxide has been added, the temperature of the reaction mixture is raised to 75°C and held there from three to four hours until the unreacted amine is less than 2.0%. The solution is cooled and the unreacted hydrogen peroxide can be destroyed by addition of a stoichiometric amount of sodium bisulfite. This may not be desirable if a low colored product is desired, ia which case residual amounts of hydrogen peroxide enhance long-term color stabiUty. 

A method suitable for analysis of sulfur dioxide in ambient air and sensitive to 0.003—5 ppm involves aspirating a measured air sample through a solution of potassium or sodium tetrachloromercurate, with the resultant formation of a dichlorosulfitomercurate. Ethylenediaminetetraacetic acid (EDTA) disodium salt is added to this solution to complex heavy metals which can interfere by oxidation of the sulfur dioxide. The sample is also treated with 0.6 wt % sulfamic acid to destroy any nitrite anions. Then the sample is treated with formaldehyde and specially purified acid-bleached rosaniline containing phosphoric acid to control pH. This reacts with the dichlorosulfitomercurate to form an intensely colored rosaniline—methanesulfonic acid. The pH of the solution is adjusted to 1.6 0.1 with phosphoric acid, and the absorbance is read spectrophotometricaHy at 548 nm (273). 

STPP = sodium tripolyphosphate NTA = nitnlotriacetic acid EDTA = ethylenediaminetetraacetic acid EDTPO = ethylenediaminetetra(methylenephosphonic acid) (see Table 1). 

From this material, samples are cut and swelled to constant weight in a buffered saline solution prepared from 8.43 g sodium chloride (NaCl), 9.26 g boric acid (H3BO3), 1.0 g sodium borate (Na3B03), and 0.1 g of the disodium salt of the dihydrate of ethylenediaminetetraacetic acid [Na2 EDTA -(/ 0)21 ini L of distilled water. 

The sodium and calcium salts of EDTA (ethylenediaminetetraacetic acid, Fig. 9.3.1.) are common sequestrants in food products. A three-dimensional representation of EDTA is shown in color Fig. 9.3.2. The EDTA ion is an especially effective sequestrant, forming up to six coordinate covalent bonds with a metal ion. These bonds are so named because a lone pair of electrons on a single atom serves as the source of the shared electrons in the bond between the metal ion and EDTA. The two nitrogen atoms in the amino groups and the oxygen... 

Standard EDTA Solutions. Di sodium dihydrogen ethylenediaminetetraacetate dihydrate is available commercially of analytical reagent purity. After drying at 80°C for at least 24 hr, its composition agrees exactly with the dihydrate formula (molecular weight 372.25). It may be weighed directly. If an additional check on the concentration is required, it may be standardized by titration with nearly neutralized zinc chloride or zinc sulfate solution. 

Corynantheidol (255) has been prepared by Hanaoka et al. (155), who started from piperideine derivative 268 and tryptophyl bromide (197). The key cyclization step, resulting in indolo[2,3-a]quinolizine 270 as the major product besides 271, was carried out by mercuric acetate oxidation in the presence of the disodium salt of ethylenediaminetetraacetic acid (EDTA), followed by sodium borohydride reduction. Finally, lithium aluminum hydride reduction of 270 provided ( )-corynantheidol in good yield (155). 

List of Abbreviations cDNA, complementary DNA ddH20, double-distilled H2O dNTP, deoxyribonu-cleotide triphosphate EDTA, ethylenediaminetetraacetic acid MgCl2, magnesium chloride mRNA, messenger ribonucleic acid NaOH, sodium hydroxide PCR, polymerase chain reaction qRT PCR, quantitative reverse transcriptase polymerase chain reaction RNase, ribonuclease RT PCR, reverse transcriptase polymerase chain reaction UTR, untranslated region... 

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