Recombination, homologous

HMG CoA-Reductase HMG-CoA-Reductase Inhibitors Homologous Desensitization Homologous Proteins Homologous Recombination Homology Modeling Hormonal Contraceptives Hormone Replacement Therapy (HRT)... 

De Lozanne, A. Spudich, J.A. (1987). Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science 236, 1086-1091. 

Gene knockouts were performed by homologous recombination in mice. The enzymes are characterized as neuronal, inducible (macrophage), and endothelial because these were the sites in which they were first identified. However, all three enzymes have been found in other sites, and the neuronal enzyme is also inducible. Each gene has been cloned, and its chromosomal location in humans has been determined. 

Ohtsubo Y, M Shimura, M Delawary, K Kimbara, M Takagi, T Kudo, AOhta, Y Nagata (2003) Novel approach to the improvement of biphenyl and polychlorinated biphenyl degradation activity promoter implantation by homologous recombination. Appl Environ Microbiol 69 146-153. 

Each of the -6000 PCR products was then co-transformed into yeast along with the recipient vector that had been linearized using a restriction enzyme that digests the plasmid at the desired cloning site. The 70 bp of homologous flanking sequence on each end of the PCR products is sufficient for the yeast homologous recombination system to act upon and insert the PCR product into the vector (Hudson et al., 1997 Ma et al., 1987). 

Figure 4.7. In vivo recombinational cloning in yeast. Two successive PCR reactions are performed. Each set of primers contains 5 flanking sequences that are eventually used for homologous recombination with vector sequences after transformation of yeast with the 2nd PCR fragment and the linearized vector.

Simple plants Physcomitrella patens Chlamydomonas reinhardtii Lemna minor Containment, clonal propagation, batch consistency, secretion of proteins into medium, regulatory compliance, homologous recombination in Physcomitrella Scalability... 

Homologous Two DNA sequences that are very similar or identical. Homologous recombination occurs between two genes that have very similar or identical sequences. Nonhomologous Two DNA sequences that are very different. Nonhomologous recombination can occur between two unrelated genes. 

ALIGNED, HOMOLOGOUS RECOMBINATION swaps information between the same genes on two copies of the same chromosome. Genes are not lost or duplicated, nor is their order changed. Different combination of specific alleles (copies of same gene) does occur. 

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