Scanning electron microscopy image analysis

Keywords tissue scaffolds, mercury porosimetry, capillary flow porometry, scanning electron microscopy, image analysis... 

Table 4.1 Screen Parameters from Scanning Electron Microscopy Image Analysis...

Table 4.2 Comparison of Three Methods for Determining the Effective Pore Diameter 1. Fit to Equation (3.20) Using Pure Fluid Contact Angles, 2. Historical Data, 3. Scanning Electron Microscopy Image Analysis...

Lab-on-a-Chip Devices for Chemical Analysis, Fig. 3 (a) Layout of the resonance cavity for an array of 16 FP optical filters (b) scanning electron microscopy image showing the cross section of one of the FP optical filters... 

Field emission scanning electron microscopy (FESEM) analysis was performed with a HITACHI S-4800 instrument in a cryo-mode with secondary electron image resolution of 1.0-1.4 nm at voltages of 1-15 kV. For all measurements, aqueous solutions of nanogels with a concentration of 5 mg/mL were rapidly vitrified in liquid propane and transferred to the high vacuum Balzers BF freeze-etching chamber. Fracture surfaces were prepared by means of a lever and etched by sublimation of the vitrified water for 5-15 min. 

Fig. 6 Scanning electron microscopy images of L929 mouse fibroblast attachment to a P3HB films, and b P3HB films plasticized with 30% BTHC after 5 days of culture [72]. P3HB/BTHC films show limited ceU adhesion, and cells exhibit a spherical, nonviable morphology. The low cell viability of these films was confirmed by the quantitative analysis (see Fig. 6). Scale bar 20 xm. c L929 mouse fibroblast attachment to P3HB fibers after 9 days of culture [72]. Scale bar 200 jjim...

Boyde, A. 1970. Practical problems and methods in three-dimensional analysis of scanning electron microscopy images. In Published Proceedings of the Third Annual Scanning Electron Microscopy Symposium. IIT Research Institute, Chicago. Pp. 107-12. 

The nascent morphology of polyethylene granules made in a reactor can be investigated by scanning electron microscopy. Competent analysis of such images can yield information regarding catalyst activity and polymerization conditions. Such analyses may be combined with energy-dispersive spectroscopy to aid in the identification of the class of catalyst used. 

Physical testing appHcations and methods for fibrous materials are reviewed in the Hterature (101—103) and are generally appHcable to polyester fibers. Microscopic analyses by optical or scanning electron microscopy are useful for evaluating fiber parameters including size, shape, uniformity, and surface characteristics. Computerized image analysis is often used to quantify and evaluate these parameters for quaUty control. 

Core-shell colloidal crystal films were prepared in three steps as outlined in Table 4.2. First, spherical submicron polystyrene particles were prepared by known methods38 39. The size of isolated polystyrene beads was 326 5 nm as determined by analysis of scanning electron microscopy (SEM) images using standard techniques. 

Thiel, B.L. (2004). Imaging and analysis in materials science by low vacuum scanning electron microscopy. International Materials Reviews 49 109-122. 

Image analysis has been used to characterize the pore structure of synthetic membrane materials. The Celgard films have also been characterized by scanning tunneling microscopy, atomic force microscopy, and field emission scanning electron microscopy. The pore size of the Celgard membranes can also be calculated from eq 5, once the MacMullin number and gurley values are known. 

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