Rabbit chondrocytes

Ascorbic acid stimulates proliferation of rabbit chondrocytes at high cell density, chick embryo and bovine articular chondrocytes, and rabbit cartilage explants. Although evidence suggests that ascorbic acid treatment does not directly stimulate transcription of ECM products, cultured chondrocytes undergo changes in gene expression characteristic of hypertrophy. 

Malemud CJ, Stevenson S, Mehraban F et al. (1994) The proteoglycan synthesis repertoire of rabbit chondrocytes maintained in type II collagen gels. Osteoarthritis and Cartilage 2 29-42... 

IGFs are measured in plasma or serum by a variety of methods including in vitro bioassay, radioreceptor assay, or immunoassay techniques.Most bioassays measure the sum of IGF-I and IGF-II bioactivity using rabbit chondrocytes, chick pelvic cartilage, or porcine rib cartilage." Such assays are relatively imprecise, insensitive, and often not specific. 

Porous poly(DL-lactide) Cell viability was Rabbit chondrocytes Cells grown on PDLLA/chitosan PDLLA/chitosan Wu et al. 

Poly(L-lactide) (PLLA) microspheres using chitosan on the surface Bioactivity study on control PLLA and the chitosan-coated PLLA microspheres by in vitro culture Rabbit chondrocytes Larger amount of chitosan-coated PLLA microspheres exhibited enhanced cell attachment and proliferation Chitosan-coated PLLA microspheres may serve as injectable cell microcarriers for chondrogenesis in cartilage tissue Lao et al. [105]... 

Choi S J, Na K, Kim S, Woo D G, Sun B K, Chung H M and Park K H (2007), Combination of ascorbate and growth factor (tgf beta-3) in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes for neocartilage formation , JBiomed Mater Res A, 83, 897-905. 

Na, K., Kim, S., Woo, D. G., Sun, B. K., Yang, H. N., Chung, H. M. Park, K. H. (2007b) Synergistic effect of TGFbeta-3 on chondrogenic differentiation of rabbit chondrocytes in thermo-reversible hydrogel constructs blended with hyaluronic acid by in vivo test. J Biotechnol, 128, 412-22. 

Yun, K. Moon, H.T. 2008, Inducing chondrogenic differentiation in injectable hydrogels embedded with rabbit chondrocytes and growth factor for neocartilage formation . Journal of Bioscience and Bioengineering, vol. 105, no. 2, pp. 122-126. 

Green Jr. W.T. Articular cartilage repair Behavior of rabbit chondrocytes during tissue culture and subsequent allografting. 1977 Clinical Orthopaedics and Related Research 124, 237. 

Fukuda, K., K. Ohtani, F. Matsumura, and S. Tanaka. 1993. Keratan sulfate inhibits its release in rabbit chondrocyte. Conn. Tiss. Res. 30 75-83. 

EDI), and water to produce a group of biodegradable PU foams. The interconnected pores varied in size from 10 to 2 mm in diameter. Rabbit bone-marrow stromal cells cultured on the materials for up to 30 days formed multilayers of confluent cells and were phenotypically similar to those grown on tissue culture PS. It supported the adherence and proliferation of both bone-marrow stromal cells and chondrocytes in vitro. In subdermal implants the investigators found that the material showed infiltration of both vascular cells and connective tissue. 

Grandolfo M, Calabrese A, D Andrea P. Mechanism of mechanically induced intercellular calcium waves in rabbit articular chondrocytes and in HIG-82 synovial cells. / Bone Miner Res. 1998 13 443-453. 

A6. Ashton, I. K., and Pocock, A. E., Action of multiplication-stimulating activity on [3H]thymidine incorporation in rabbit and human fetal chondrocytes in vitro. J. Endocrinol. 99, 93-98 (1983). 

P14. Postel-Vinay, M. C., Corvol, M. T., Lang, F., Fraud, F., Guyda, H., and Posner, B. 1., Receptors fi>r insulin-like growth fiictors in rabbit articular and growth plate chondrocytes in culture. Exp. Cell Res. 148, 105-116 (1983). 

Bone Toxicity. Beta emissions from radiostrontium bound to bone resulted in various bone lesions (trabecular osteoporosis, sclerosis, osteolytic lesions), particularly in animals that were exposed chronically (Book et al. 1982 Clarke et al. 1972 Momeni et al. 1976). In young rats and rabbits exposed orally to 90Sr, necrotic effects on the vasculature of developing bone secondarily disrupted the process of osteogenesis (Casarett et al. 1962 Downie et al. 1959). Disruption in the metaphyseal microvasculature disorganized the transformation of cartilage into bone, so that chondrocytes inappropriately resumed active proliferation. 

Zheng, Z. Bei, F. F. Xian, H. L. and Chen, G. Q. Effects of crystallization of poly-hydroxyaUsanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes. Biomater. 2005, 26, 3537-3548. 

Biosynthesis. — Up to 56% of sulphated glycosaminoglycans synthesized by explant cultures of human and rabbit articular chondrocytes are located in the trypsin-digestible pericellular coat. ° Sulphate is incorporated into both D-glucopyranosyluronic acid and L-idopyranosyluronic acid residues in hybrid glycosaminoglycans by monolayers of chick embryo arterial fibroblasts. Several oligosaccharides, released on treatment with chondroitin lyase ABC and chondroitin lyase AC, have been characterized. 

Deng Y, Zhao K, Zhang XF, Hu P, Chen GQ (2002) Study on the three-dimentional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalktmoate scaffolds. Biomaterials 23 4049-4056... 

---