Polymerase chain reaction amplification reactions

C. E. Gustafson, C. J. Thomas, and T, J. Trust, Detection of Aera/tm/tav-salmoni-cida from fish by using polymerase chain-reaction amplification of the virulence surface array protein gene. Appl. Environ. Microbiol., 5S 3825 (1992). 

Iqbal, S. Robinson, J. Deere, D. Saunders, J. R. Edwards, C. Porter, J. Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water. Lett. Appl. Microbiol. 1997,24,498-502. 

Sato Y, Sugie R, Tsuchiya B, et al. Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalin-fixed and paraffin-embedded tissues. Diagn. Mol. Pathol. 2001 10 265-271. 

Fig. 7.3 Polymerase chain reaction amplification of part of the phaseolin genes surrounding the 15 repeat. 1 T phaseolin (Andean) 2 S phaseolin (Mesoamerican) 3 1 phaseolin (Ecuador and northern Peru) (Kami et al. 1995)...

Price T, Aitken J, Simpson ER. 1992. Relative expression of aromatase cytochrome P450 in human fetal tissues as determined by competitive polymerase chain reaction amplification. J Clin Endocrinol Metab 74 879-883. 

As the NNRTIs are structurally diverse and yet bind to RT at a common site, the similar occurrence of resistance-conferring mutations is not surprising. As a consequence, the effectiveness of other NNRTIs may be compromised by the emergence of HIV-1 variants caused by a previous NNRTI therapy (Sardana et ah, 1992). Experiments were performed in which HIV-1 strains (JR-CSF or ME) are cultured in human lymphocytes in the presence of partially inhibitory concentrations of delavirdine (Dueweke et al., 1993b). These conditions yield mutants that are 100-fold resistant. In order to determine what mutation(s) occurs, PCR (polymerase chain reaction) amplification and DNA sequence analysis of the RT coding region were applied and indicated that mutation P236L had occurred. Mutations at amino acids 181 or 183, which have been associated with resistance to other NNRTIs, are not detected. 

From R. H. Liu, J. Yang, R. Lenigk, J. Bonanno, and R Grodzinskl, "Self-Contained, Fully Integrated Biochip tor Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection." Anal. 

Baumann, R. E., and Henschen, A. H. (1993). Human fibrinogen polymorphic site analysis by restriction endonuclease digestion and allele-specific polymerase chain reaction amplification Identification of polymorphisms at positions A alpha 312 and B beta 448. Blood 82, 2117-2124. 

Moskaluk, C. A., and Kern, S. E. 1997. Microdissection and polymerase chain reaction amplification of genomic DNA from histological tissue sections. Am. J. Pathol. 750 1547-1552. 

Liu, R. H., Yang, J., Lenigk, R., Bonanno, J., Grodzinski, P., Self-contained, hilly integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection, Anal. Chem. 2004, 76, 1824-1831. 

Nuovo GJ, Gallery F, MacConnell P, Becker J, Bloch W. An improved technique for the in situ detection of DNA after polymerase chain reaction amplification. Am J Pathol 1991 139 1239-1244. 

Marks JD, Tristem, Karpas A, Winter G, Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes, Eur. J. Immunol., 21 985-991, 1991. 

A conclusive diagnosis of G6PD deficiency can be made by DNA analysis. This is of particular use when the most likely variants in a population are known since polymerase chain reaction amplification of the appropriate region of the G6PD gene can be performed and the mutation identified. 

Oda RP, Strausbauch MA, Huhmer AF, Borson N, Jurrens SR, Craighead J, et al. Infrared-mediated thermocycling for ultrafast polymerase chain reaction amplification of DNA. Anal Chem 1998 70 4361-4368. 

Huhmer AF, Landers JP. Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Anal Chem 2000 72 5507-5512. 

Manos MM, Ting Y, Wright DK, et al. The use of polymerase chain reaction amplification for tire detection of genital human papillomaviruses. Cancer Cells 1989 7 209-14. 

Culture incubation on suitable media for 4 to 10 days Polymerase chain reaction amplification of specific DNA sequences... 

Chan, W.K. and R.H. Devlin. Polymerase chain reaction amplification and functional characterisation of sockeye salmon histone H3, metallothionein-B, and protamine promoters. Mol. Mar. Biol. Biotechnol. 2 308-318, 1993. 

Kai M, Wada I, Imai S, Sakane F, Kanoh H (1996) Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes. Polymerase chain reaction amplification of mouse H2O2-inducible hic53 clone yielded the cDNA encoding phosphatidic acid phosphatase. J Biol Chem 271 18931-18938... 

E2. Eisenach, K. D., Cave, M. D., Bates, J. H., and Crawford, J. T., Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J. Infect. 

Olive, D. M., Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase. J. Clin. Microbiol. 27, 261-265 (1989). 

Zietkiewicz, E. et al.. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176, 1994. 

Lasota, J., Jasinski, M. ot al. (1998) Detection of ihe SYT-SSX fusion transcripts in formaldehyde-fixed, paraffin embedded tissue a reverse transcriptase polymerase chain reaction amplification assay useful in the diagnosis of synovial sarcoma. Mod Pathol, 11, 626-633. 

Molor, R.J., Meeker, T.C., Wittwer, C.T. (1994) Antigen expression and polymerase chain reaction amplification of mantle cell lymphoma. Blood, 83, 1626—1631. 

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