Integrated Biochips

Figure 26-40 Integrated biochip for DNA analysis of biological samples. Dimensions are 100 x 60 x 2 mm. Two bright circles are piezoelectric vibrators for ultrasonic agitation. 

From R. H. Liu, J. Yang, R. Lenigk, J. Bonanno, and R Grodzinskl, "Self-Contained, Fully Integrated Biochip tor Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection." Anal. 

Liu, R. H., Yang, J., Lenigk, R., Bonanno, J., Grodzinski, P., Self-contained, hilly integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection, Anal. Chem. 2004, 76, 1824-1831. 

H.F. Cui, J.S. Ye, Y. Chen, S.C. Chong, X. Liu, T.M. Lim and F.S. Sheu, In situ temporal detection of dopamine exocytosis from 1-dopa-incubated nm9d cells using microelectrode array-integrated biochip, Sensors and Actuators B-Chemical, 115(2), 634-641 (2006). 

Biomedical microdevices Blood analysis Drug delivery Integrated biochips i-STAT... 

A Total Integrated Biochip System for Detection of SNP in Cancer... 

In this paper we report a total integrated BIOCHIP system for rapid detection and identification of SNPs in cancer. The system consists of a disposable biochip and a control/ actuation unit that can realize the detection of SNPs and point mutations in K-ras and TP3 gene in an automated fashion. This means that the genotyping answer can be obtained for the individual patient in 4-5 hours compared to at present where the patient may have to wait up to several weeks since at the hospital samples from several patients are collected and the procedure only perform 1-2 times a month. 

Vo-Dinh T., Alarie J.P., Isola N., Landis D., Wintenberg A.L., Ericson M.N., DNA biochip using a pliototransistor integrated circuit, Anal. Chem. 1998 71 358-363. 

T. Basarsky, Overview of a microarray scanner design essentials for an integrated acquisition and analysis platform, in Microarray Biochip Technology (M. Schena, ed.), p. 265, Eaton Publishing (2000). 

Figure 1.19 Molecular diagnostics. The future of the biochip rests on its ability to serve as a fully integrated device for multiplexed analysis.

Other extensions that have been made include the construction of a biochip in which a reverse transcription PCRprocess can be carried out [384] and the integration of PCR with capillary electrophoresis [385], DNA microarray hybridization [386] and sample preparation [387,388]. The speed of analysis, the ease of integrating different functions and the relative low costs of micro-PCR production are important advantages of the miniaturized PCR technique that suggest that this bioorganic microreactor device will be thoroughly implemented in many analytical labs. 

---