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Restriction endonucleases digestion

FIGURE 11.33 Restricdon mapping of a DNA molecule as determined by an analysis of the electrophoretic pattern obtained for different restriction endonuclease digests. (Keep in mind that a dsDNA molecule has a unique nucleotide sequence and therefore a definite polarity thus, fragments from one end are distinctly different from fragments derived from the other end.)... [Pg.354]

Figure 40-2. Results of restriction endonuclease digestion. Digestion with a restriction endonuclease can result in the formation of DNA fragments with sticky, or cohesive, ends (A) or blunt ends (B).This is an important consideration in devising cloning strategies. Figure 40-2. Results of restriction endonuclease digestion. Digestion with a restriction endonuclease can result in the formation of DNA fragments with sticky, or cohesive, ends (A) or blunt ends (B).This is an important consideration in devising cloning strategies.
Baumann, R. E., and Henschen, A. H. (1993). Human fibrinogen polymorphic site analysis by restriction endonuclease digestion and allele-specific polymerase chain reaction amplification Identification of polymorphisms at positions A alpha 312 and B beta 448. Blood 82, 2117-2124. [Pg.285]

Restriction endonuclease digest compatible with cleavage site of vector repaired ends fir added linker... [Pg.162]

Day 1 Restriction Endonuclease Digestion of pUC19 and pUC4K, Ethanol Precipitation of DNA Fragments, and DNA Ligation... [Pg.351]

Day 4 Restriction Endonuclease Digestion and Agarose-Gel Electrophoresis of Plasmid DNA... [Pg.354]

Peerbooms PG, Kuijt P, Maclaren DM (1987) Application of chromosomal restriction endonuclease digest analysis for use as typing method for Clostridium difficile. J Chn Pathol 40 771-776 Pirttijiarvi TS, Andersson MA, Salkinoja-Salonen MS (2000) Properties of Bacillus cereus and other bacilli contaminating biomaterial-based industrial processes. Int J Food Microbiol 60 231-239... [Pg.209]

Restriction of one or two hairpins to expose sticky ends. Cohesive ends are liberated by the controlled removal of DNA hairpins utilizing restriction endonuclease digestion. [Pg.426]

Earlier reports on the use of MALDI-TOF-MS for the detection of mutahons and/or sequence changes have employed more conventional techniques such as restriction endonuclease digests [146] or the ligase chain reaction [147]. Inihally, allele-specific hybridization was also combined with mass spectrometric detection. Smith and coworkers, for example, used pephde NA probes hybridized against an immobilized PCR template for genotyping [148]. Finally, the direct measurement of PCR products has been attempted as a means of NA analysis and genotyping [17, 24, 92, 149, 150]. [Pg.193]

Fractionation of restriction endonuclease digests of plasmid and bacteriophage DNA 202... [Pg.13]

Restriction endonuclease digests of DNA are routinely analysed on agarose or 202... [Pg.217]

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See also in sourсe #XX -- [ Pg.15 , Pg.391 ]



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