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Multiwell plates live cells

Live cells, which overexpress the protein/ ycoprotein of interest, can form excellent targets for antibody selection when grovm either in suspension or as adherent monolayers in multiwell plates. For example, tumour cells that overexpress grotvth factor receptors, or Chinese hamster ovary cells expressing recombinant human proteins, or insect cells expressing baculovirus constructs. Also, such cell lines can be used to target intracellular proteins after fixation and permeabilization of the ceUs. [Pg.16]

When canying out ELISAs on live cells it is not necessajy to lyse the cells after the substrate has been added and the coloured product formed can be read directly from the multiwell plate. [Pg.17]

Transfer 50 (jlI aliquots of the mixtures to the cells or antigen coated PVC multiwell plate and incubate for 1 h at 4°C (live cells) or at ambient temperatme (PVC plates). [Pg.21]

Assay using antigen coated PS or PVC coated multiwell plates 15 Assay using live or fixed adherent cells grown in multiwell plates 16 Ligand-binding assays for anti-receptor antibcxlies 17... [Pg.503]

Assay Using Live Adherent Cells Grown in Multiwell PS Plates... [Pg.31]

See other pages where Multiwell plates live cells is mentioned: [Pg.59]    [Pg.733]    [Pg.503]    [Pg.329]    [Pg.21]    [Pg.222]    [Pg.21]    [Pg.373]    [Pg.729]   
See also in sourсe #XX -- [ Pg.16 ]



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