Magnets plate

Plate Magnets. Plate magnets (Fig. 6a) are simple devices usually mounted in the bottom of a chute or duct. These magnetized plates are manufactured in various models and are normally of the permanent magnet type. The largest units provide protection to about 115 mm of material depth, at chute angles of up to 45° from the horizontal. 

SPRI plate. The multiwelled plates move through vacuum manifolds, shakers, heating blocks, and magnetic plate shelf. Gripper arms and conveyer belts are used as necessary. 

In order to preserve the high temperature of the lipid hydrating solution in the screw-capped bottle, a 50-mL beaker with water is placed on the hot (magnetic) plate and the botde is placed in the beaker (see Fig. 2). The water in the beaker is occasionally gendy stirred and re-filled, to compensate for evaporation. 

Twenty-four well plate format magnetic plate (OZ Biosciences, France or chemicell, Germany)... 

To evaluate the effect of magnetic field targeting of folate targeted magnetic liposomes, the culture plate with the respective samples is positioned on a 24 well format magnetic plate. 

As described before, cells incubated with magnetic liposome formulations are positioned on a 24 well format magnetic plate during the first hour of incubation to see the effects of magnetic field on targeting. 

To evaluate the cytoxicity of folate targeted magnetic liposomes under permanent magnetic field, the plate with magnetic liposomes is put on a 96 well format magnetic plate for the first hour of incubation by another hour incubation without magnetic field (total 2 h incubation). 

Magnets magnetic plate (magnetic plate OZ Biosciences). 

To sediment magnetic transfection complexes, place the plate on the magnetic plate for 30 min. 

Place the cell culture plate on a magnetic plate for 15-30 min to create a permanent magnetic fleld at the cell layer with a field strength and gradient of 70-250 mT and 50-130 T/m, respectively. 

Remove the magnetic plates afrer 20-30 min exposure of the cells to the magnetic field (see Note 19), and incubate the plate containing the transfected cells in a cell culture incubator at 37°C in a 5% CO atmosphere until evaluation. 

Centrifuging the plates, before adding the transfection complexes to ensure that the cells are at the bottom of the plate. This is important to enable the transport of the transfection complexes to the cell membrane, under the influence of the magnetic force during incubation at the magnetic plate. 

The optimal incubation conditions and tiie optimal exposure time at the magnetic plate to sediment the transfection complexes at the cell membrane may differ from one cell type (or transfection complex) to another and must be determined experimentally. 

Fig. y.3.y I KIc I i [nside-oul NMR the entire NMR speclromcter is lowered inlo a bore hole. Data are acquired while the device is pulled up with constant velocity. The cross-.sectii>n through the probe shows that a region of homogeneous field outside the device is generated by two permanent magnet plates with parallel polarization. A half coaxial antenna is used for excitation... 

In a similar implementation, an electromagnetic actuator was introduced in Judy and Muller (1996, 1997). This actuator consists of a torsional polysilicon beam, firmly fixed at both ends to the substrate, and an attached magnetic plate made of NiFe at the middle of the beam. When a magnetic field is applied by an electromagnet, a pure moment is induced and the magnetic plate is rotated out of plane (Figure 2.11). The electromagnet is actually a 10-turn coil... 

---