Methylene chloride, solubility

An aqueous extract of P. hysterophorus (collected in Puerto Rico) was partitioned into methylene chloride at pH 7, pH 10 and pH 2. Bioassays of the methylene chloride soluble fractions, using the bean second internode bioassay (13), showed that the highest activity was concentrated in the methylene chloride extract at pH 7. Extensive chromatographic purification (flash chromatography, medium pressure LC, preparative TLC) monitored by bioassay led to the isolation of the four sesquiter-... 

Data on c clohexene and a pinene aerosols were reported by Schwartz after a preliminary report from the Battelle Institute group. The experimental conditions and analytic techniques were identical with those just described for the toluene aerosol study. Here again, only the methylene chloride-soluble, water-insoluble fractions were studied. They accounted for about 7% and 65% of the total aerosol mass generated from cyclohexene and a pinene, respectively. Grosjean (unpublished data) has investigated the chemical composition of cyclopentene, cyclohexene, and 1,7-octadiene aerosols. Experiments were conducted in an 80-m Teflon smog chamber filled with ambient air, with irradiation by... 

Soluble in ethanol, benzene, carbon tetrachloride, chloroform, ether, and methylene chloride Solubility in water ... 

We have previously reported (18, 19) the Isolation of herltol (I) from the methylene chloride soluble portion of the chloroform-soluble Fraction B (Scheme I). 

The acetone extracts were diluted 2 1 with water and partitioned against methylene chloride. The methylene chloride-soluble extracts were analyzed by comparative TLC (X). The aqueous phases were concentrated to dryness and dissolved in water that contained 5% acetonitrile and 0.2> acetic acid. These solutions were applied to SEP-PAK cartridges (Waters Associates) and the cartridges were washed with 5-10 ml of water and eluted with 5 ml of 50t acetonitrile. From 90 to 95% of the 1 C was recovered from the cartridges in the 50% acetonitrile eluate. The eluates were concentrated to dryness and dissolved in 18% acetonitrile/1% acetic acid and subjected to HPLC as previously described for PCNB metabolism in peanut roots (6). 

Methylene chloride-soluble residues. Methylene chloride-or chloroform-soluble C-labeled products were major residues in all of the plant tissues examined except peanut cell ciiltures (Figure 3). Chloroform-soluble C accounted for 59.2 of the radioactivity isolated from peanut roots 48 hr after treatment with [ C]PCNB. The radioactivity was in the form of PCNB (28.7 ), pentachloroaniline (22.5 ), pentachlorothiophenol (2.6 ) pentachlorothloanlsole (3.1 ) pentachlorothloanlsole sulfoxide (0.5 ) S-(pentachlorophenyl)-2-thioaoetic acid [(S-(PCP)ThioAcetate] (0.5 ) and S-(pentachlorophenyl)-3-thio-2-hydroxypropionic acid [S-(PCP)ThioLactate] (0.2 ) and S-(PCP)Cys (trace) (J), The structures of these compounds are shown in Figure 13. Based on TLC, the last three compounds in this list were classified as polar chloroform- or methylene chloride-soluble residues and the remaining compounds were classified as nonpolar residues. 

Methylene chloride-soluble radioactivity accounted for only 4-551 of the applied In peanut cell cultures harvested 3 days after treatment with [ CjPCNB. This value remained fairly constant between 3 days and 14 days. Pentachlorothloanlsole and pentachloroanlllne accounted for 1.1)1 and 3.951 of the applied In the cultures 24 hr after treatment. Pentachloroanlllne Increased to 10.5)1 of the applied In cultures that were not shaken during the 24-hr treatment, Indicating that pentachloroanlllne formation was favored under conditions of low oxygen tension. The enzymatic formation of pentachloroanlllne from PCNB In the presence of NADPH, PAD, and an enzyme from peanut occured only under anaerobic conditions IT). 

Polar methylene chloride-soluble residues. Polar methylene chloride-soluble residues were found In most of the plant tissues treated with [ C]PCNB (Figure 14). These products were only Identified In peanut IT). The polar methylene chloride-soluble metabolites from peanut, S-(PCP)Cys, S-(PCP)ThloAcetate, and S-(PCP)ThloLactate, were probably produced from S-(PCP)GSH by the pathway shown In Figure 15. Intact peanut plants treated with S-[( C)PCP]Cys and harvested 20 days later yielded S-(( C)-PCP]ThloAcetate In T.3% yield however, S-(( C)PCP]ThloLactate was not detected. An S-substltuted 2-thloacetlc acid metabolite has also been reported In the metabolism of EPTC In the rat ( 1 ). 

Nonpolar methylene chloride-soluble residues. Pentachloro-thloanisole and pentachlorothioanlsole sulfoxide were present in the nonpolar methylene chloride-soluble fraction from each of the plant systems examined (Figure 14). In addition, pentachloro-thiophenol was detected in some of these extracts. Pentachloro-thioanisole has been reported as an important residue of PCNB in almost every biological system that has been examined for PCNB metabolism and pentachlorothlophenol has also been reported as a residue in several of these systems S). The formation of these residues from S-(PCP)GSH via the pathway shown in Figure 16 was considered highly probable. Recent vivo studies indicated that such a system also operates in mammals in the metabolism of propachlor ( ) and pentachlorothioanlsole (20). vitro... 

Colorless gas pungent suffocating odor density 2.975 g/L fumes in moist air liquefies at -101°C sohdifies at -126.8° vapor pressure at -128°C is 57.8 torr critical temperature -12.2°C critical pressure 49.15 atm critical volume 115 cm3/mol soluble in water with partial hydrolysis solubdity in water at 0°C 332 g/lOOg also soluble in benzene, toluene, hexane, chloroform and methylene chloride soluble in anhydrous concentrated sulfuric acid. 

The methylene chloride soluble portion of a methanol extract from the wood of Eury-coma longifolia shows strong cytotoxic properties. One of the active constituents, longi-lene peroxide (77) (P2i, 0—0 = 1.472 A, Figure 31), was isolated in a 0.0003% yield. The characteristic horse-shoe-like arrangement, which is observed in the crystal structure of squalene derivative 77, has been associated with its biological activity ... 

Analysis of endogenous IAA. The extracts of cells with methanol were subjected to solvent fractionation using methylene chloride to give methylene chloride soluble acidic fractions. The fractions were purified by HPLC on an ODS column and finally by HPLC on a Nucleosil 5N(CH3>2 column with a fluorescent detector. Quantification was made by using an internal standard of indole-3-propionic acid which was added before purification. The results are shown in Figure 7. 

Dimers are a class of compounds isolated from only seven species of Celastraceae family [8,88]. These triterpene dimers were found to be composed of one quinoid type and one aromatic type of triterpene derived from pristimerin, tingenone and / or their congeners joined together by two ether linkages formed between the two A rings or between the A and B rings. From the methylene chloride soluble portion of a methanolic extract of Maytenus chuchuhuasca, four dimeric compounds were isolated by Shirota et al. [88]. 

The soluble and insoluble products were analyzed for elemental content of carbon, hydrogen, nitrogen and oxygen with Perkin-Elmer 240 series instruments. The methylene chloride soluble oil product was also analyzed as a methylene chloride solution on a gas... 

Results from the liquefaction experiments with the five molst-blomass feedstocks are given In Table III. The oil yield Is based on the combined mass of acetone- and methylenechlorlde-soluble oils as a percent of the mass of dried feedstock calculated to an ashfree basis. The product oil elemental analysis Is the calculated composite analysis for the combined acetone- and methylene chloride-soluble oils. 

Macrocycle Vila readily forms the doubly deprotonated nickel complex upon addition of Ni(OAc)2. The methylene chloride soluble complex was unreactive under reaction conditions similar to those employed in the nickel cyclam/PhIO system. However, oxidation of - 8-methylstyrene occurred readily when the hypochlorite phase transfer conditions were used. Epoxidation represented about 50% of the reaction pathway with the remainder yielding a mixture of benzaldehyde and other over-oxidation products. 

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