Matrix-assisted laser desorption/ionization diseases

Wattiez R et al. Human bronchoalveolar lavage fluid protein two-dimensional database study of interstitial lung diseases. Electrophoresis 2000 21 2703-2712. Yanagida M et al. Matrix assisted laser desorption/ionization-time of flight-mass spectrometry analysis of proteins detected by anti-phosphotyrosine antibody on two-dimensional-gels of fibrolast cell lysates after tumor necrosis factor-alpha stimulation. Electrophoresis 2000 21 1890-1898. 

Many diseases are characterized by the expression of specific proteins1 in some cases, malignant cells yield unique protein profiles when total cellular protein extracts are analyzed by proteomic methods such as two-dimensional gel electrophoresis or matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS).2 High-throughput proteomic studies may be useful to differentiate normal cells from cancer cells, to identify and define the use of biomarkers for specific cancers, and to characterize the clinical course of disease. Proteomics can also be used to isolate and characterize potential drug targets and to evaluate the efficacy of treatments. 

Kim J, Kim SH, Lee SU et al. Proteome analysis of human liver tumor tissue by two-dimensional gel eleetrophoresis and matrix-assisted laser desorption/ionization-mass spectrometry for identification of disease-related proteins. Electrophoresis 2002 23 4142 156. 

T. Fujiwaki, M. Tasaka, N. Takahashi, H. Kobayashi, Y. Murakami, T. Shimada, and S. Yamaguchi, Quantitative evaluation of sphingolipids using delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry with sphingosylphosphorylcholine as an internal standard. Practical application to cardiac valves from a patient with Fabry disease, J. Chromatogr. B. 832 (2006) 97-102. 

Snel M, Fuller M (2010) High-spatial resolution matrix-assisted laser desorption ionization imaging analysis of glucosylceramide in spleen sections from a mouse model of gaucher disease. Anal Chem 82(9) 3664-3670. doi 10.1021/ac902939k... 

Ekstrom, S., Wallman, L., Malm, J., Becker, C., LUja, H., Laurell, T., and Marko-Varga, G, Integrated selective enrichment target—A microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases. Electrophoresis, 25, 3769-3777, 2004. 

Ruh, H., Sandhoff, R., Meyer, B., Gretz, N., and Hopf, C. 2013. Quantitative characterization of tissue globotetraosylceramides in a rat model of polycystic kidney disease by primadrop sample preparation and indirect high-performance thin layer chromatography-matrix-assisted laser desorption/ionization-time-of flight-mass spectrometry with automated data acquisition. Anal. Chem., 85 6233-6240. 

Taki, T. 2012. An approach to glycobiology from glycolipidomics Ganglioside molecular scanning in the brains of patients with Alzheimer s disease by TLC-blot/ matrix assisted laser desorption/ionization-time of flight MS, Biol Pharm. Bull, 35 1642-1647. 

Figure 11.5. ) One of the major components in brain matter are lipids, and changes in phospholipid concentrations in tissue are associated with Farber disease, Alzheimer disease, and Gaucher disease. Therefore, by profiling the rat brain lipid metabolism using IM-MS, early detection could be beneficial for prevention or intervention of these diseases. The focus of this work was measuring lipids in rat brain tissue using a low-pressure matrix-assisted laser desorption/ionization (MALDI)-low pressure IM-MS and determining noncovalent complexes between lipids in the tissue and chlorisondamine, a nicotinic antagonist.

Sud M, Fahy E, Cotter D, et al. LMSD LIPID MAPS structure database. Nucleic Acids Res. 2007 35 D527-D532. Tajima Y, Ishikawa M, Maekawa K, et al. Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer s disease. Lipids Health Dis. 2013 12 68. Woods AS, Jackson SN. Brain tissue lipidomics direct probing using matrix-assisted laser desorption/ ionization mass spectrometry. AAPS J. 2006 8(2) E391-395. 

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