Integrated Selective Enrichment

Ekstrom, S., Wallman, L., Malm, J., Becker, C., LUja, H., Laurell, T., and Marko-Varga, G, Integrated selective enrichment target—A microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases. Electrophoresis, 25, 3769-3777, 2004. 

The functionality of the silicon nanovial target plates was extended by the development of so-called integrated selective enrichment target (ISET) technology. The ISET device contains... 

Ekstrom S, Wallman L, Hok D, Marko-Varga G, Laurell T (2006) Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target. J Proteome Res 5 1071-1081... 

An integrated module for on-line sample pre-analytical treatment and/or clean-up could be also included in the device. With this respect, field-flow fractionation (FFF) techniques, which can separate analytes based on their morphological characteristics (size, shape and superficial properties) can be exploited to develop pre-analytical modules for cells or macromolecules (e.g., proteins, protein complexes or adducts) fractionation, thus providing a selectively enriched fraction for the analysis. 

The stereogenic centers may be integral parts of the reactants, but chiral auxiliaries can also be used to impart facial diastereoselectivity and permit eventual isolation of enantiomerically enriched product. Alternatively, use of chiral Lewis acids as catalysts can also achieve facial selectivity. Although the general principles of control of the stereochemistry of aldol addition reactions have been well developed for simple molecules, the application of the principles to more complex molecules and the... 

An integrated microfabricated cell sorter has been constructed using a control layer and a fluidic layer fabricated on PDMS [890]. The control layer consists of valves that will be pneumatically controlled by pressurized N2. This device is superior to an electrokinetic sorter, which suffers from buffer incompatibilities and frequent voltage adjustments because of ion depletion or pressure imbalance due to evaporation. E. coli cells expressing EGFP were sorted and selected out from a cell mixture which also contained E. coli cells expressing the / -nitrobenzyl (PNB) esterase. About 480,000 cells have been sorted at a rate of 44 cefls/s in 3 h. The recovery yield was 40% and the enrichment ratio was about 83-fold [890]. 

Mattow J, Siejak F, Hagens K et al (2007) An improved strategy for selective and efficient enrichment of integral plasma membrane proteins of mycobacteria. Proteomics 7 1687-1701... 

Integral Flow In many systems flow plays an integral role in structuring the separation process. The essential character of the separation method therefore hinges on the existence, orientation, and nature of flow. Most commonly the flow—which is inherently nonselective—serves to magnify a local enrichment induced by the selective forces underlying /i. ... 

---