Biomarker protein

Holland, R. D. Rafii, F. Heinze, T. M. Sutherland, J. B. Voorhees, K. J. Lay J. O., Jr. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric detection of bacterial biomarker proteins isolated from contaminated water, lettuce and cotton cloth. Rapid Comm. Mass Spectrom. 2000,14, 911-917. [Pg.149]

Figure 12.1. Different MS strategies for identification of biomarker proteins. (See color insert.)...

In addition to MALDI-TOF and LC-MS/MS, SELDI-TOF-MS can also be used to determine expression profiling of various biological samples, such as serum or plasma for early detection of infection. Serum proteomic profiling assay, for instance, has been used to distinguish patients with acute SARS (severe acute respiratory syndrome) from patients with fever and influenza with 100% accuracy [16]. A major limitation of SELDI-TOF-MS, however, is that it cannot be used for direct amino acid sequence identification of the biomarker proteins, necessitating further sample fractionation and protein purification. [Pg.271]

Nanoscience-Based Electrochemical Sensors and Arrays for Detection of Cancer Biomarker Proteins... [Pg.1]

The next section discusses the nature and significance of biomarker proteins for cancer, followed by a section reviewing the use of nanoparticles in sensors and detection protocols. The section following discusses the combination of nanoscience-assisted sensing with microfluidics for multiplexed protein detection. We end the chapter with an overview and comments on the future of cancer diagnostics based on biomarker detection. [Pg.2]

Many proteins are overexpressed and secreted into the blood beginning at very early stages of developing cancers. Serum levels of these proteins can indicate cancer and guide therapy even before the onset of detectable tumors. Biomarker proteins are often specific to several types of cancer, and panels of such proteins promise a more much reliable assessment of patient status than single biomarkers [2, 5, 8, 16, 17]. The most famous clinically used single biomarker protein is... [Pg.2]

This chapter summarizes recent progress in development of ultrasensitive electrochemical devices to measure cancer biomarker proteins. The emphasis is on the use of nanoparticles and nanostructured sensors aimed for use in clinical cancer... [Pg.19]

The next important issue is the choice of the biomarker protein panel. Reliability of the panels for diagnostics needs to be quantitatively examined by smdies... [Pg.20]

Rusling, J.F., Kumar, C.V., Gutkind, J.S., et al. Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer. Analyst 135, 2496-2511 (2010)... [Pg.21]

Rusling, J. F., Sotzing, G., Papadimitrakopoulos, F. Designing nanomaterials-enhanced electrochemical immunosensors for cancer biomarker proteins, Bioelectrochem. 76, 189-194 (2009)... [Pg.24]

Sardesai, N.P., Barron, J.C., Rusting, LF. Carbon nanotube microwell array for sensitive electrochemilumineseent detection of cancer biomarker proteins Anal. Chem. 83, 6698-6703 (2011)... [Pg.26]

Jensen, G.C., Krause, C.E., Sotzing, G.A., Rusling, J.F. Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarker protein. Phys. Chem. Chem. Phys. 13, 4888 894 (2011)... [Pg.26]

Tang, C.K., Vaze, A., Rusling, J.F. Fabrication of immunosensor microwell arrays from gold compact discs for ultrasensitive detection of cancer biomarker proteins. Lab on a Chip (2011, in press). doi 10.1039/ClLC20833K... [Pg.26]

Mani, V., Chikkaveeraiah, B.V., Patel, V., Gutkind, J.S., Rusting, J.F. Ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-paiticle amplification. ACS Nano 3, 585-594 (2009). doi 10.1021/nn800863w... [Pg.101]

This book provides a comprehensive compilation of seven chapters, with important contributions of several authors. Chapter 1 reviews the recent research in using nanoparticle labels and multiplexed detection in protein immunosensors. This chapter summarizes recent progress in development of ultrasensitive electrochemical devices to measure cancer biomarker proteins, with emphasis on the use of nanoparticles and nanostmctured sensors aimed for use in clinical cancer diagnostics. Based on recent strategies focused on nanomaterials for electrochemical biosensors development. Chap. 2 discusses the development of new... [Pg.141]

FIGURE 19.1 Schematic illustrating whole-cell mass spectrometry. The intact bacterial cell is crystallized within the matrix, and intact biomarker proteins are ionized during the MALDI process, and are detected by TOF-MS [8]. [Pg.425]

In order to evaluate the proteomic profile of whole cells and to correctly identify proteins based solely on the intact mass, advanced instrumentation offering high mass accuracy is necessary. The application of Fourier transform mass spectrometry (FTMS) to intact cell analysis allowed for unambiguous identification of biomarker proteins due to the high resolution and mass... [Pg.427]

Set-up and publish lists of organism-specific biomarkers (proteins or peptides) as a community resource for quick and reliable identification... [Pg.295]

The presence of certain biomarker proteins and/or irregular protein concentrations is a sign of cancer and other disease states. Sensitive, convenient, and precise proteinsensing methods provide crucial tools for the early diagnosis of diseases and successful treatment of patients. Recently, the chemical nose approach was developed, which provides an alternative for the sensing protocols that use exclusive analyte-receptor binding pairs as its basis. The strategy for the creation of protein sensors is to use the particle surface for protein... [Pg.3617]

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