Matrix-assisted laser desorption instrumentation

For non-volatile sample molecules, other ionisation methods must be used, namely desorption/ionisation (DI) and nebulisation ionisation methods. In DI, the unifying aspect is the rapid addition of energy into a condensed-phase sample, with subsequent generation and release of ions into the mass analyser. In El and Cl, the processes of volatilisation and ionisation are distinct and separable in DI, they are intimately associated. In nebulisation ionisation, such as ESP or TSP, an aerosol spray is used at some stage to separate sample molecules and/or ions from the solvent liquid that carries them into the source of the mass spectrometer. Less volatile but thermally stable compounds can be thermally vaporised in the direct inlet probe (DIP) situated close to the ionising molecular beam. This DIP is standard equipment on most instruments an El spectrum results. Techniques that extend the utility of mass spectrometry to the least volatile and more labile organic molecules include FD, EHD, surface ionisation (SIMS, FAB) and matrix-assisted laser desorption (MALD) as the last... 

R. S. Bordoli, K. Howes, R. G. Vickers, R. H. Bateman, and D. J. Harvey. Matrix-Assisted Laser Desorption Mass Spectrometry on a Magnetic Sector Instrument Fitted with an Array Detector. Rapid Commun. Mass Spectrom., 8(1994) 585-589. 

API offers unique opportunities for the implementation of new sources or to develop new applications. Atmospheric pressure matrix assisted laser desorption (AP-MALDI) [21] can be mounted on instruments such as ion traps which were originally designed only for electrospray and LC-MS. New API desorption techniques such as desorption electrospray (DESI) [22] or direct analysis in real time (DART) [23] have been described and offer unique opportunities for the analysis of surfaces or of solid samples. 

ToF mass spectrometers as dynamic instruments gained popularity with the introduction of matrix assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) as effective pulsed ion sources for the soft ionization of large biomolecules (up to 10s dalton) due to their high ion transmission.38 ToF mass spectrometers, quadrupole analyzers and/or magnetic sector fields can be combined in tandem mass spectrometers (MS/MS) for the analysis of organic compounds. 

Mass spectrometry has played a role in biochemistry since the early 1940s when it was introduced for use in following isotopic labels during metabolism.199-20 However, it was not until the 1990s that suitable commercial instruments were developed to permit mass spectrometry using two new methods of ionization. The techniques are called matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry. 

Electrospray ionization and matrix-assisted desorption ionization were both introduced around the same time, in the late 1980s. In fact matrix-assisted laser desorption ionization (MALDI) was first mentioned in the literature in 1987 (Karas et al., 1987). In the years prior to that, there were limited reports of the application of laser desorption MS. Early developments in MALDI focused primarily on macromolecules, particularly peptides and proteins. Historically, MALDI ion sources have predominantly been coupled to time-of-flight (TOF) instruments. TOF requires precise timed ionization events, and since ions are generated in MALDI by a pulsed desorption, this combination is complementary. Mass spectra generated by MALDI can be relatively simple, containing predominantly singly charged ions. The importance of both ESI and MALDI are well proven in the analysis of biomolecules, and both techniques were awarded the Nobel Prize for chemistry in 2002 (Chapter 1). 

Mass spectrometers are used not only to detect the masses of proteins and peptides, but also to identify the proteins, to compare patterns of proteins and peptides, and to scan tissue sections for specific masses. MS is able to do this by giving the mass-to-charge ratio of an ionized species as well as its relative abundance. For biological sample analysis, mass spectrometers are connected to an ionizing source, which is usually matrix-assisted laser desorption ionization (MALDI) [14], surface-enhanced laser desorption/ioni-zation (SELDI, a modified form of MALDI) [15], or electrospray ionization [16]. These interfaces enable the transfer of the peptides or proteins from the solid or liquid phase, respectively, to the gas (vacuum) phase inside the mass spectrometer. Both MALDI and electrospray ionization can be connected to different types of mass analyzers, such as quadrupole, quadruple-ion-traps, time of flight (TOF), or hybrid instruments such as quadrupole-TOF or Fourier transform-ion cyclotron resonance. Each of these instruments can... 

Developments in mass spectrometry technology, together with the availability of extensive DNA and protein sequence databases and software tools for data mining, has made possible rapid and sensitive mass spectrometry-based procedures for protein identification. Two basic types of mass spectrometers are commonly used for this purpose Matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF) mass spectrometry (MS) and electrospray ionization (ESI)-MS. MALDI-TOF instruments are now quite common in biochemistry laboratories and are very simple to use, requiring no special training. ESI instruments, usually coupled to capillary/nanoLC systems, are more complex and require expert operators. We will therefore focus on the use of MALDI-... 

Stoeckli, M. Farmer, T. B. Caprioli, R. M. 1999. Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument. J. Am. Soc. Mass Spectrom., 10, 67-71. 

Figure 6 The SELDI technology. This type of proteomic analytical tool is a class of mass spectroscopy instrument that is useful in high-throughput proteomic fingerprinting of serum. Using a robotic sample dispenser, 1 p,L of serum is applied to the surface of a protein-binding chip. A subset of the proteins in the sample binds to the surface of the chip. The bound proteins are treated with a matrix-assisted laser desorption and ionization matrix and are washed and dried. The chip, which contains multiple patient samples, is inserted into a vacuum chamber where it is irradiated with a laser. The laser desorbs the adherent proteins and causes them to be launched as ions. The TOF of the ion before detection by an electrode is a measure of the mass-to-charge (m/z) value of the ion. The ion spectra can be analyzed by computer-assisted tools that classify a subset of the spectra by characteristic patterns of relative intensity (adapted from www.evmsdoctors.com).

Naven T, Harvey D, Brown J, Critchley G (1997) Fragmentation of complex carbohydrates following ionization by matrix-assisted laser desorption with an instrument fitted with time-lag focusing. Rapid Commun Mass Spectrom 11 1681-1686... 

The critical aspect of this otherwise simple instrument is the need to produce the ions at an accurately known start time and position. These constraints generally limit TOF spectrometers to use pulsed ionization techniques, which include plasma and laser desorption (e.g., MALDI, matrix assisted laser desorption ionization). 

Two techniques that have become preffered for ionisation of proteins/peptides is electrospray ionisation (ESI) and matrix-assisted laser desorption/ionisation (MALDI). Although different combinations of ionisation techniques and mass analyser exist, MALDI usually is coupled with a time-of-flight (TOF) (Figure 7) tube as a mass analyser while ESI is tradionally combined with quadrupole mass analysers. Instruments capable of MS/MS have the ability to select ions of particular m/z ratio from a mixture, to fragment selected ions and to record the precise masses of the resulting fragment ions. If this process is applied to the analysis of peptide ions, in principle the amino acid sequence of the peptide can be deduced. 

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