Laboratory biochemistry

Laboratory Biochemistry of Reproduction Oudwijk 11, 3581 TE Utrecht The Netherlands... 

The November 2001 issue of the Journal of Chemical Education ipp 1533-1534) describes an introductory biochemistry laboratory ex periment involving cycio hexanone monooxygenase oxidation of cyclic ketones... 

Ben May Laboratory for Cancer Research and Departments of Chemistry and Biochemistry University of Chicago Chicago, Illinois... 

Aldehydes and ketones are among the most important of ail compounds, both in biochemistry and in the chemical industry. AUdehydes are normally prepared in the laboratory by oxidation of primary alcohols or by partial reduction of esters. Ketones are similarly prepared by oxidation of secondary alcohols or by addition of diorganocopper reagents to acid chlorides. 

NCMH Physical Biochemistry Laboratory, University of Nottingham,... 

Hughes, GJ. and Frutier, S. 1990 Amino acid analysis protocols, possibilities, and pretensions. In Fini, C, Floridid, A., Finelli, V.N. and Wittman-Leibold, B., eds., Laboratory Methodology in Biochemistry Amino Acid Analysis and Protein Sequencing. Boca Raton, FL, CRC Press, Inc. 44-61. 

Scientist, Research Instimte, Hospital for Sick Children, Toronto, and Associate Professor, Departments of Laboratory Medicine and Pathobiology and Department of Biochemistry, University of Toronto... 

C18-0119. In a biochemistry laboratory, you are asked to prepare a buffer solution to be used as a solvent for isolation of an enzyme. On the shelf are the following solutions, all 1.00 M formic acid (Za = 1.8 X 10 ), acetic acid = 1.8 x 10 ), sodium formate (NaHC02), and sodium acetate (NaCH3 CO2). Describe how you would prepare 1.0 L of a pH = 4.80 buffer solution... 

The energy provision by carbohydrate metabolism has been extensively studied Ihm the beginning of this century, chiefly in an attempt to understand the basic biochemistry of alcohol production from carbohydrafe. However, many laboratory culture media contain only nitrogenous compounds and their metabolism is of importance as it clearly provides energy for growth and maintenance. 

Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh, Groningen, The Netherlands... 

Rice-Evans, C.A., Diplock, A.T. and Symons, M.C.R. (1991). Mechanisms of radical production. In Laboratory Techniques in Biochemistry and Molecular Biology , Vol. 22, Techniques in Free Radical Research , (eds. R.H. Burdon and P.H. van Knippenberg) pp. 19-50. Elsevier, Amsterdam. 

The aim of this review was to summarize those aspects of fluorescence spectroscopy that may have value for solving problems in food science and technology. The techniques described, which are mainly based on front-face fluorescence spectroscopy coupled with multidimensional statistical methods, have been illustrated by examples taken from the literature and the work done in our laboratory. Although fluorescence spectroscopy is a technique whose theory and methodology have been extensively exploited for studies of both chemistry and biochemistry, the utility of fluorescence spectroscopy for molecular studies has not yet been fully recognized in food science. Fluorescence spectroscopy has the same potential to address molecular problems in food science as in the biochemical science field, because the scientific questions that need to be answered are closely related. We hope that this coverage will introduce a novel class of techniques in the emulsion and gel fields. 

Department of Chemistry and Biochemistry, Materials Department, Materials Research Laboratory, and Department of Chemical Engineering University of California, Santa Barbara, CA, 93106... 

KRISTI LEW is a former high school science teacher with degrees in biochemistry and genetics. After years in the classroom and cytogenetics laboratories, she decided that she wanted to write full-time. She is the author of more than 20 nonfiction science books for students and teachers. 

Richard C. Honeycutt, Ph.D., was born in Newport News, VA, in 1945. He attended Anderson University in Anderson, IN, from 1963 to 1967 and earned an A.B. in Chemistry. He received his Ph.D. in Biochemistry from Purdue University in 1971 and served as a Postdoctoral Fellow from 1971 to 1973 at the Smithsonian Institution s Radiation Biology Laboratory. Dr. Honeycutt worked as a Senior Chemist at Rohm and Haas Company from 1973 to 1976 and as a Senior Metabolism Chemist at Ciba Geigy from 1976 to 1989. Currently, he is President of the Hazard Evaluation and Regulatory Affairs Company, Inc., which he founded in 1990, and is an analytical biochemist and field research specialist/consultant engaged in exposure assessment of pesticides to humans and the environment. 

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