Lupin proteins

Lupin proteins have been analyzed by a number of different techniques, including liquid chromatography (Duranti et al., 1995), differential sedimentation (Franco et al., 1997 Freitas et al., 2000), proteomic analysis by one- and two-dimensional gel electrophoresis (Duranti et al., 1992 Magni et al., 2005b) or liquid chromatogra-phy/electrospray ionization tandem mass spectrometry (Schwend et al., 2003 Wait et al., 2005 Magni et al., 2007), isoelectrofocusing (Quaresma et al., 2007), crystalb-zation (Biesiadka et al., 1999), and expression of recombinant protein (Scarafoni et al., 2001). 

Until recently the detection of lupine protein was based on the use of serum from lupin-allergic patients and performed by immunoblot and radioallergosorbent test (RAST) analyses (Figure 22.10). The standardization of serum is difficult, and limited availability makes such methods unsuitable for routine analysis. Recently, a sandwich... 

FIGURE22.8 Iinmunoblot on lupin protein extract (L. aftMj )usingseraof 11 patients who are sensitized to lupin. Ml, M2, molecular-weight markers L, lupin extract (Coomassie-stained SDS-gel) 1-11, patient sera. (From Faeste et al., unpublished data.)... 

FIGURE 22.10 (a) Immunoblot using polyclonal anti-lupin antibody (1, molecular-weight marker 2, L. aftMj-derived extract 3, L. angustifolius flour extract) (b) Representative linear six-point calibration curve based on the standard curve obtained using the lupin protein standard in the sandwich ELISA. (From Holden etal., 2005.) (Reprinted with permission from Reference Citation. Copyright 2005 American Chemical Society.)... 

Furthermore, a novel polyclonal/monoclonal-based sandwich ELISA for the detection of lupin proteins in foods was described (Holden et al., 2007). The monoclonal antibody reacts specifically with a-conglutin (Dooper et al., 2007). The assay has a detection limit of Img protein/kg food and is sensitive to both native and processed proteins from L. angustifolius and L. albus. A selection of 112 food samples, both with and without lupin declaration, was analyzed showing that the majority of products were labeled correctly. 

D Agostina A, Antonioni C, Resta D, et al. (2006). Optimization of a pilot-scale process for producing lupin protein isolates with valuable technological properties and minimum thermal damage. J. Agric. Food. Chem., 54 92-98. 

Guarneri E, Guarneri C, Benvenga S (2005). Identiflcation of potentially cross-reactive peanut-lupine proteins by computer-assisted search for amino acid sequence homology. Int. Arch. Allergy Immunol, 138 273-277. 

Wasche A, Muller K, Knauf U (2001). New processing of lupin protein isolates and functional properties. Nahrung, 45 393-395. 

HA. See Hydroquinone monomethyl ether Haarlem oil. See Cade oil Haematoxylin. See Hematoxylin Haematoxylon campechianum Haematoxylon campechianum extract. See Logwood (Haematoxylon campechianum) extract Hairactive . See Hydrolyzed lupine protein Hairfix. See... 

Hydrolyzed lecithin. See Lysolecithin Hydrolyzed lupine protein CAS 84082-55-3 EINECS/ELINCS 282-001-8 Synonyms Lupine protein hydrolysate Definition Hydrolysate of lupine protein derived by acid, enzyme, or other hydroiysis Uses Antistat, hair conditioner, skin conditioner in cosmetics protective sunscreen for skin care... 

Lupine protein hydrolysate. See Hydrolyzed lupine protein... 

Structuring. See Hydrolyzed lupine protein Struktoi 6PPD. See N-(1,3-Dimethylbutyl)-N -phenyl-p-phenylenediamine Struktoi Caicium Stearate. See Calcium st0d rst0... 

Hydrolyzed DNA Hydrolyzed fibronectin Hydrolyzed hemoglobin Hydrolyzed lupine protein Hydrolyzed milk protein Hydrolyzed oat flour Hydrolyzed oats Hydrolyzed pea protein Hydrolyzed placental protein Hydrolyzed potato protein Hydrolyzed reticulin Hydrolyzed rice protein Hydrolyzed RNA Hydrolyzed serum protein Hydrolyzed silk ethyl ester Hydrolyzed soy protein Hydrolyzed sweet almond protein Hydrolyzed wheat protein hydroxypropyl polysiloxane Hydrolyzed yeast Hydroxyethyl cetyidimonium phosphate... 

Diglycol/CHDM/isophthalates/SIP copolymer sunscreen protectant, skin care Hydrolyzed lupine protein sunscreen vehicle, creams C12-15 alkyl benzoate sunscreen vehicle, oils C12-15 alkyl benzoate sunscreen, anti-UV-A Pansy (Viola tricolor) extract sunscreen, cosmetics TEA-salicylate sunscreen, topical p-Aminobenzoic acid Cinnamal sunscreening agent, cosmetics Guanine Homosalate suntan lamp monitors Thallium (I) chloride suntan lotions 3-Methylumbelliferone suntan preps. 

In a 90-day feeding study, rats were fed with diets supplemented with 20 % lupin protein from sweet varieties of L. albus and L. luteus, containing 0.05 % (0.5 g kg ) and 0.09 % (0.9 g kg ) of lupin alkaloids, respectively. The actual levels of alkaloids present in the diets were not reported, but taking into account the protein levels in sweet lupins of 35 % and 39 % and assuming full incorporation of the alkaloids into the diet, the lupin flour dietary levels would amount to about 290 and 460 mg alkaloids kg diet, respectively, resulting in dietary intakes of about 14.5 and 23 mg lupin alkaloids kg bw day . No adverse effects were observed in terms of food intake, organ weight, and microscopic examinations [43]. 

Franco, J.M., Raymundo, A., Sousa, 1. and Gallegos, C. (1998) Influence of processing variables on the rheological and textural properties of lupin protein-stahiUzed emnlsions. /. Agric. Food... 

Yoshie-Stark Y, Wasche A. 2004. In Vitro Binding of Bile Adds by Lupin Protein Isolates and Their Hydrolysates. Food Chemistry 88(2) 179-184. 

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