Lupin proteins detection

Until recently the detection of lupine protein was based on the use of serum from lupin-allergic patients and performed by immunoblot and radioallergosorbent test (RAST) analyses (Figure 22.10). The standardization of serum is difficult, and limited availability makes such methods unsuitable for routine analysis. Recently, a sandwich... 

Furthermore, a novel polyclonal/monoclonal-based sandwich ELISA for the detection of lupin proteins in foods was described (Holden et al., 2007). The monoclonal antibody reacts specifically with a-conglutin (Dooper et al., 2007). The assay has a detection limit of Img protein/kg food and is sensitive to both native and processed proteins from L. angustifolius and L. albus. A selection of 112 food samples, both with and without lupin declaration, was analyzed showing that the majority of products were labeled correctly. 

Mellenthin O, Galena R. Analysis of polyphenols using capillary zone electrophoresis and HPLC detection of soy, lupin and pea protein in meat products. J Agric Food Chem 1999 47 594-602. 

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