Reverse-phase HPLC performance liquid chromatography

The aim of the work is investigate possibilities of application of Cartridges Packed DIAPAK for concentrating antibiotics Cefazoline and Levomycetine and analyze them by Reversed Phase High Performance Liquid Chromatography (RP HPLC). 

Reversed-phase high-performance liquid chromatography (HPLC) column 50 mm x 3.2-mm i.d. with Kromasil 5- um Gig packing High-performance liquid chromatograph coupled to a triple-quadrupole mass spectrometer with an atmospheric pressure chemical ionization (APCI) source Gel permeation chromatograph with a 60 mm x 25-mm i.d. column packed with Bio-Beads SX-3 (50-g)... 

RP-HPLC Reversed-phase high performance liquid chromatography TFA Trifluoroacetic acid... 

The ELISA can be used as one component of a battery of analyses. Rarely is only one method used in isolation. Other tests include chromatographic methods such as reversed-phase high-performance liquid chromatography (HPLC), size exclusion chromatography, and physical structure analytical methods such as UV spectral analysis, mass spectroscopy, etc. 

Lipophilicity represents the affinity of a molecule or a moiety for a lipophilic environment. It is commonly measured by its distribuHon behaviour in a biphasic system, either liquid-liquid (e.g. partition coefficient in 1-octanol/water) or solid-liquid (retention on reversed-phase high-performance liquid chromatography (RP-HPLC) or thin-layer chromatography (TLC) system). 

Bartke, N., Fischbeck, A., Humpf, H. U. (2006). Analysis of sphingolipids in potatoes Solanum tuberosum L.) and sweet potatoes Ipomoea batatas (L.) Lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESl-MS/MS). Mol. Nutr. Food Res., 50,1201-1211. 

The chemical properties of HVA, 5HIAA and 3-MD make them amenable to reverse-phase high-performance liquid chromatography (HPLC) with electrochemical detection. Furthermore, the composition of CSF means that little, if any, sample preparation is required prior to analysis. However, the susceptibility of these metabolites to oxidation means that careful sample collection and storage is required in order to minimise analyte degradation. 

We have followed the pathway of TNT degradation in the consortium using reverse phase high performance liquid chromatography (HPLC) and mass spectroscopy (MS). Analysis of the supernatant in the consortium showed sequential reduction of TNT to TAT however, TAT only occasionally accumulated, and even then only in very low concentrations. In addition, methylphloroglucinol (MPG) and />-cresol transiently appeared in the supernatant. All compounds were identified using HPLC by UV spectra and retention times as compared to authentic standards chromatographed under the same conditions. The mono- and diamino intermediates were also confirmed by the MS results. 

Reversed-phase high-performance liquid chromatography (RP-HPLC), cholesterol measurement, 456-458 Rhamnogalacturonanas, 735 Rice bran oil, tocopherols/tocotrienols in, 488 (figs.)... 

In this unit, methods for reversed-phase high-performance liquid chromatography (HPLC) are described for the analysis of polyphenolics. HPLC analysis can be employed in an easy and fast manner to obtain an accurate elucidation and quantification of individual polyphenolic compounds found in plant-based materials. The separation of each polyphenolic is based on the polarity differences among polyphenolics with structural similarities and uses various combinations of mobile and stationary phases. 

I de Noni, G de Bernardi, L Pellegrino. Detection of common-wheat (Triticum aestivum) flour in Durum-wheat (Triticum durum) semolina by reverse-phase high-performance liquid chromatography (RP-HPLC) of specific albumins. Food Chem 51 325-329, 1994. 

---