Reverse-phase high-pressure liquid chromatography

J. Korpi, D. P. Wittmer, B. J. Sandman, and W. C. Haney, Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high-pressure liquid chromatography Reversed-phase, ion-pair approach, J. Pharm. Sci., 65 1087 (1976). 

High-pressure liquid chromatography, reversed phase (HPLC, RP) ... 

High Pressure Liquid Chromatography.—Reverse phase h.p.l.c. using octadecyl bonded groups has been developed for the rapid and efficient separation of nucleosides, nucleotides, and protected oligonucleotides on both analytical and preparative scales. ... 

The stationary phase matrices used in classic column chromatography are spongy materials whose compress-ibihty hmits flow of the mobile phase. High-pressure liquid chromatography (HPLC) employs incompressible silica or alumina microbeads as the stationary phase and pressures of up to a few thousand psi. Incompressible matrices permit both high flow rates and enhanced resolution. HPLC can resolve complex mixtures of Upids or peptides whose properties differ only slightly. Reversed-phase HPLC exploits a hydrophobic stationary phase of... 

During, A. et al., Assay of P-carotene 15,15-dioxygenase activity by reverse phase high-pressure liquid chromatography, Anal. Biochem., 241, 199, 1996. 

Bennett, H.P.J. Browne, C.A. Goltzman, D. and Solomon, S. Isolation of peptide hormones by reverse-phase high pressure liquid chromatography. In Gross, E., and Meinenhofer, J., ed, Pepti des. Structure and Bioloai cal Function. Rockford,... 

Unger, S. H. Cook, J. R. Hollenberg, J. S., Simple procedure for determining octanol-aqueous partition, distribution, and ionization coefficients by reverse-phase high-pressure liquid chromatography, J. Pharm. Sci. 67, 1364—1366 (1978). 

Reverse Phase High-Pressure Liquid Chromatography of Halcino-nide. Progesterone is used as internal standard. 

J. A. Montgomery, T. P. Johnston, H. J. Thomas, J. R. Piper, and C. Temple, Jr., The use of microparticu-late reversed-phase packing in high pressure liquid chromatography of compounds of biological interest. 

JN Thompson, WB Maxwell. Reverse-phase high-pressure liquid chromatography of vitamin A in margarine, infant formula, and fortified milk. J Assoc Off Anal Chem 60 766-771, 1977. 

DS Smyly, BB Woodward, EC Conrad. Determination of saccharin, sodium benzoate and caffeine in beverages by reverse phase high pressure liquid chromatography. J Assoc Off Anal Chem 59(1) 14-19, 1976. 

A series of closely related oligosaccharides with increasing complexity and in decreasing abundance were observed on reverse-phase high pressure liquid chromatography as the peracetylated derivatives [procedure adapted from those of Wells and Lester... 

Oxyphenbutazone, and other non-narcotic analgesics are screened by reverse-phase high-pressure liquid chromatography (39). The column is LiChrosorb RP8 and the mobile phase is a acetonitrile-water mixture. Blood and organs are homogenized in 4% perchloric acid before extraction with the solvents. Urine is extracted without primary treatment. 

The removal of unmodified RNA fragments following the dye-labeling step is accomplished by reverse-phase high-pressure liquid chromatography (HPLC) (Fig. 2.4B) (Walter, 2003). Reversed-phase HPLC uses a column... 

B Flouret, D Mengin-Lecreulx, J van Heijenoort. Reverse-phase high-pressure liquid chromatography of uridine diphosphate N-acetylmuramyl peptide precursors of bacterial cell wall peptidoglycan. Anal Biochem 114 59-63, 1981. 

A. Richard and G. Andermann, Simultaneous determination of atropine sulfate and tropic acid by reversed-phase high pressure liquid chromatography, Pharmazie, 39 866 (1984). 

Various workers [23-32] have studied the application of high performance liquid chromatography to the determination of PAHs in water samples. Hagenmaier et al. [31] used a reversed phase high-pressure liquid chromatography procedure for the determination of trace amounts of polycyclic aromatic hydrocarbons in water. Different column packing materials were tested, in conjunction with non polar stationary phases of various polarities, for separation efficiency, detection limits and long-term stability. The method was suitable for concentrations as low as 2ng L 1 in a IL sample. Compounds... 

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