Reverse-phase liquid chromatography copper

Figure 8.43 Separation of enantiomers using complexation chromatography. A, Separation of alkyloxiranes on a 42 m x 0.2S mm I.O. open tubular column coated with 0.06 M Mn(II) bis-3-(pentafluoro-propionyl)-IR-camphorate in OV-ioi at 40 C. B, Separation of D,L-amino acids by reversed-phase liquid chromatography using a mobile phase containing 0.005 M L-histidine methyl ester and 0.0025 M copper sulfate in an ammonium acetate buffer at pH 5.5. A stepwise gradient using increasing amounts of acetonitrile was used for this separation.

SH Lee, TS Oh, HW Hae. Enantiomeric separation of free amino acids using Af-alkyl-L-proline copper (II) complex as chiral mobile phase additive in reversed phase liquid chromatography. Bull Korean Chem Soc 13 280-285, 1992. 

Mills, G.L., Hanson, A.K., Quinn, 3.G. and Lammela, W.L., 1982. Chemical studies of copper-organic complexes Isolated from estuarine waters using Ci8 reverse-phase liquid chromatography. Mar. Chem., 11 355-377. 

Mills, G. L., and Quinn, J. G. (1981). Isolation of dissolved organic matter and dissolved copper-organic complexes from estuarine waters using reverse-phase liquid chromatography. Mar. Chem. 10, 93-102. 

Liquid chromatography-absorption spectrophotometry was used by Vlacil and Hamplova [281] for the determination of lead and copper in natural waters. The metal diethyldithiocarbamates are extracted and concentrated by evaporation, followed by reversed phase liquid chromatography of the chelates. The copper and lead chelates can also be sequentially detected by spectrophotometry at 440 and 280nm. The detection limits for copper and lead were 8.6 and 17pg L 1 respectively, when liquid chromatography was used, and were 58 and 17pg L respectively when spectrophotometry was used. 

The chromatographic procedure intially investigated was reverse-phase liquid chromatography (RPLC) using Cig-bonded silica gel. This technique has been used previously for the preconcentration of trace metals from seawater prior to analysis by atomic absorption spectrophotometry (23, 24) and for the isolation of organically bound copper from seawater (4). These methods were modified and adapted for automation. 

THE OFF-LINE DETERMINATION OF COPPER AS A DITHIOCARBAMATE COMPLEX BY REVERSED-PHASE LIQUID CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION... 

Before considering the special requirements for automated on-line determination of metals from industrial effluents, it is worthwhile examining the features of standard laboratory procedures associated with the off-line determination of copper as a dithiocarbamate complex by liquid chromatography with electrochemical detection. The off-line determination of copper as its diethyldithiocarbamate complex in aqueous samples, zinc plant electrol3d e, and urine have been described [3, 7, 10] using reverse phase liquid chromatography with amperometric detection. A standard instrumental configuration for the conventional laboratory off-line method as used in these studies is depicted in Fig. 7.2. 

When reverse-phase liquid chromatography is used with electrochemical detection in a flowing solution, a buffer is usually present in the mobile phase. The buffer itself should be electroinactive and not interfere with the determination of copper. Instead of using 30% water (0.2 M NaNOa), a suitable aqueous component would be say 30% wa-... 

Inoue, H. et al., Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography, J. Ghromatogr. A, 679, 99, 1994. 

Further applications are described for sulfur (Fig. 2.6) and copper in water by reversed-phase or ion-exchange high-performance liquid chromatography . Also anions such as thiosulphate and polythionates were separated from environmental samples by HPLC (anion-exchange) . The sensitive detection was made possible by reaction with Ce(lV) after separation and fluorimetry of Ce(lII) ions. 

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