Reversed-phase high-pressure liquid chromatography

During, A. et al., Assay of P-carotene 15,15-dioxygenase activity by reverse phase high-pressure liquid chromatography, Anal. Biochem., 241, 199, 1996. 

Bennett, H.P.J. Browne, C.A. Goltzman, D. and Solomon, S. Isolation of peptide hormones by reverse-phase high pressure liquid chromatography. In Gross, E., and Meinenhofer, J., ed, Pepti des. Structure and Bioloai cal Function. Rockford,... 

Unger, S. H. Cook, J. R. Hollenberg, J. S., Simple procedure for determining octanol-aqueous partition, distribution, and ionization coefficients by reverse-phase high-pressure liquid chromatography, J. Pharm. Sci. 67, 1364—1366 (1978). 

Reverse Phase High-Pressure Liquid Chromatography of Halcino-nide. Progesterone is used as internal standard. 

JN Thompson, WB Maxwell. Reverse-phase high-pressure liquid chromatography of vitamin A in margarine, infant formula, and fortified milk. J Assoc Off Anal Chem 60 766-771, 1977. 

DS Smyly, BB Woodward, EC Conrad. Determination of saccharin, sodium benzoate and caffeine in beverages by reverse phase high pressure liquid chromatography. J Assoc Off Anal Chem 59(1) 14-19, 1976. 

A series of closely related oligosaccharides with increasing complexity and in decreasing abundance were observed on reverse-phase high pressure liquid chromatography as the peracetylated derivatives [procedure adapted from those of Wells and Lester... 

Oxyphenbutazone, and other non-narcotic analgesics are screened by reverse-phase high-pressure liquid chromatography (39). The column is LiChrosorb RP8 and the mobile phase is a acetonitrile-water mixture. Blood and organs are homogenized in 4% perchloric acid before extraction with the solvents. Urine is extracted without primary treatment. 

The removal of unmodified RNA fragments following the dye-labeling step is accomplished by reverse-phase high-pressure liquid chromatography (HPLC) (Fig. 2.4B) (Walter, 2003). Reversed-phase HPLC uses a column... 

B Flouret, D Mengin-Lecreulx, J van Heijenoort. Reverse-phase high-pressure liquid chromatography of uridine diphosphate N-acetylmuramyl peptide precursors of bacterial cell wall peptidoglycan. Anal Biochem 114 59-63, 1981. 

A. Richard and G. Andermann, Simultaneous determination of atropine sulfate and tropic acid by reversed-phase high pressure liquid chromatography, Pharmazie, 39 866 (1984). 

Various workers [23-32] have studied the application of high performance liquid chromatography to the determination of PAHs in water samples. Hagenmaier et al. [31] used a reversed phase high-pressure liquid chromatography procedure for the determination of trace amounts of polycyclic aromatic hydrocarbons in water. Different column packing materials were tested, in conjunction with non polar stationary phases of various polarities, for separation efficiency, detection limits and long-term stability. The method was suitable for concentrations as low as 2ng L 1 in a IL sample. Compounds... 

Reverse phase high pressure liquid chromatography (HPLC) was used to further fractionate the sample and add another dimension of specificity (8,21). The extract was evaported to dryness and taken up in CHCI3. The entire extract was injected onto a DuPont Zorbax ODS column at 40°C using 2 cc/min. CH3OH mobile phase. Typical chromatograms are shown in Figure 2. 

Arveldano, M. I., van Rollins, M., and Horrocks, L. A. (1983) Separation and quantitation of free fatty acid and fatty acid methyl esters by reverse phase high pressure liquid chromatography, J. Lipid Res. 24, 83-93. 

Powell WS (1985) Reversed-phase high-pressure liquid chromatography of arachidonic acid metabolites formed by cyclo-oxygenase and lipoxygenases. Analyt Biochem 148 59-69... 

The D4 content of the samples was determined by reverse-phase high-pressure liquid chromatography (HPLC) with a Varian 5500 liquid chromatograph. A DuPont Zorbax ODS (Cis) column was used with a Wilmad infrared detector set at 12.45 xm to monitor the Si-CHa vibration. The mobile phase was an 83 17 mixture of acetonitrile and acetone at a flow rate of 0.8 mL/min. A Rheodyne injector valve operating on compressed air was used with a 10-p,L sample loop for reproducible injection volumes. Ethyl acetate was used to dissolve the samples for analysis. 

Sithole, B.B. Guy, R.D. Separation of nitroimidazoles by reversed-phase high-pressure liquid chromatography. Talanta 1986, 33, 95-97. 

Unger, S.H., Cook, J.R. and Hollenberg, J.S. (1978). Simple Procedure for Determining Octanol-Aqueous Partition, Distribution, and Ionization Coefficients by Reversed-Phase High-Pressure Liquid Chromatography. J.Pharm.Sci., 67,1364-1367. 

Recently, it was found that chemoselective O-H insertions of dimeric a-CyD hosts by their encapsulated carbene 21 guests produced 2-0-(2-adamantanyl)a-CyD (2-25a) and 3-0- (2-ada m a n ta n y 1) a - CyD (3-25a).108 Within the cavities of /i-CyD at 30 °C, these innermolecular insertion products were isolated in 35% yield. Correspondingly, in the smaller confines of a-CyD, as much as 58% of 2- and 3-25a were formed.109 In contrast, the yield of the intramolecular insertion product 22 remains unchanged at 10%. Innermolecular insertion products 2- and 3-25a were separated via reversed-phase high-pressure liquid chromatography (RP HPLC),109... 

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