High performance liquid chromatography reversed-phase columns

Homish, R. E. and Wiest, J. R., Quantitation of spectinomycin residues in bovine tissues by ion-exchange high-performance liquid chromatography with post-column derivatization and confirmation by reversed-phase high performance chromatography-atmospheric pressure chemical ionization tandem mass spectrometry, /. Chromatogr. A, 812, 123, 1998. 

CR Krishnamurti, AM Heindze, G Galzy. Application of reversed-phase high-performance liquid chromatography using pre-column derivatization with o-phthalaldehyde for the quantitative analysis of amino acids in adult and fetal sheep plasma, animal feeds and tissues. J Chromatogr 315 321-331, 1984. 

Reversed-phase high-performance liquid chromatography (RP-HPLC) columns (C8, C18) from semipreparative (7 mm internal 0) down to capillary (<1 mm). Phase porosity of 300 A and granulometry of 7 p,m are preferred. 

Investigation of Fulvic Acid—Cu Complexation by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography with Post-Column Fluorescence Quenching Titration... 

Recently we completed an evaluation of the effectiveness of the Aflatest (Vicam) immunoaffinity column for analysis of corn, peanuts and peanut butter for aflatoxin (11). In this procedure the sample is extracted with Me0H/H20, filtered, and the extract diluted to <30% MeOH with water. An aliquot is then applied to the immunoaffinity column. The column is then washed with water and the aflatoxins eluted with methanol. Total aflatoxins are then determined by solution fluorometry with bromine (SFB), or individual toxins by reverse phase high performance liquid chromatography with post column I2 derivatization (PCD). In the collaborative study corn samples naturally contaminated with aflatoxins and samples of corn, peanuts and peanut butter spiked at 30, 20 and 10 ng/g, were analyzed by 24 collaborators. The results of this study are tabulated in Table V. 

Sun, H.-L. Liu, H.-M. Tsai, S.-J. Quantitative analysis of manganese, chromium and molybdenum by ion-pair reversed-phase high-performance liquid chromatography with pre-column derivatization and UV-visible detection. J. Chromatogr. A, 1999, 857, 351-357. 

Ion-Exchange, Hydrophobic, and Reverse Phase High-Performance Liquid Chromatography Disk Monolithic Columns... 

Takahashi, K., Fukuwatari, T., and Shibata, K., 2009. Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column deri-vatization. Journal of Chromatography B. 877 2168-2172. 

Reversed-phase Cig chromatography column. Keystone Scientific Betasil, 100 x 2.0-mm i.d., 5-pm particle size, 100 A, Part No. 105-701-2-CPF TSQ 7000 LC/MS/MS system with electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) interface and gradient high-performance liquid chromatography (HPLC) unit, or equivalent Vacuum manifold for use with SPE cartridges (Varian Vac Elut 10 or equivalent)... 

Reversed-phase high-performance liquid chromatography (HPLC) column 50 mm x 3.2-mm i.d. with Kromasil 5- um Gig packing High-performance liquid chromatograph coupled to a triple-quadrupole mass spectrometer with an atmospheric pressure chemical ionization (APCI) source Gel permeation chromatograph with a 60 mm x 25-mm i.d. column packed with Bio-Beads SX-3 (50-g)... 

Koyama, J., Nomura, J., Shiojima, Y., Ohtsu, Y., and Horii, I., Effect of column length and elution mechanism on the separation of proteins by reversed-phase high-performance liquid chromatography, /. Chromatogr., 625, 217, 1992. 

McCalley, D. V., Effect of organic solvent modifier and nature of solute on the performance of bonded silica reversed-phase columns for the analysis of strongly basic compounds by high-performance liquid chromatography, /. Chromatogr A, 738(2), 169, 1996. 

Kirkland, J. J., Henderson, J. W., DeStefano, J. J., van Straten, M. A., and Claessens, H. A., Stability of silica-based, endcapped columns with pH 7 and 11 mobile phase for reversed-phase high-performance liquid chromatography, J. Chromatogr., A, 762(1 2), 97, 1997. 

Imaz, C., Navajas, R., Carreras, D., Rodriguez, C., and Rodriguez, F.A., Comparison of various reversed-phase columns for the simultaneous determination of ephedrines in urine by high-performance liquid chromatography, /. Chromatogr. A, 870 (1 2), 23, 2000. 

R. Ricker, L. Sandoval, B. Permar, and B. Boyes, Improved reversed-phase high performance liquid chromatography columns for biopharmaceutical analysis, J. Pharmaceut. Biomed. Anal., 14, 93 (1995). 

Nimura, N., Itoh, H., Kinoshita, T., Nagae, N., Nomura, M. (1991). Fast protein separation by reversed-phase high-performance liquid-chromatography on octadecylsilyl-bonded non-porous silica-gel — effect of particle-size of column packing on column efficiency. J. Chromatogr. 585(2), 207-211. 

Tanaka, M., Yamazaki, H. (1996). Direct determination of pantoprazole enantiomers in human serum by reverse-phase high-performance liquid chromatography using a cellulose-based chiral stationary phase and column-switching system as a sample cleanup procedure. Anal. Chem. 68, 1513-16. 

Turner and Warnock [56] determined miconazole in human saliva using high performance liquid chromatography. Deproteinated human saliva samples containing miconazole was chromatographed on a C8 reversed-phase radial compression column using 77% methanol in 0.01 M EDTA with 0.005 M w-nonylamine at a flow... 

Procedure Flavonoids are then further purified with 2 ml of methanolic HC1 (2 N), followed by centrifugation (2 min, 15 600 g), hydrolyzation of 150 il of suspension in an autoclave (15 min, 120 C). A reverse osmosis-Millipore UF Plus water purification system is used in high performance liquid chromatography (HPLC) with an autosampler. After injections of 5 pg of samples, the mobile phases flow at a rate of 1 ml/minute with isocratic elution in a column at 30 C. 

---