Liquid chromatography procedures

Liquid Chromatography Procedure for Polyolefin Additives, WAPP-100, Waters, Milford, MA (1978). 

Spectrophotometric determination with 4-hexylresorcinol and a fluorometric method with m-aminophenol are the most commonly used procedures for the determination of acrolein. However, gas chromatography and high-performance liquid chromatography procedures are also used (USEPA 1980 Kissel etal. 1981 Nishikawa and Hayakawa 1986). Acrolein concentrations in rainwater between 4 and 200 pg/L can be measured rapidly (less than 80 min) without interference from related compounds the method involves acrolein bromination and analysis by gas chromatography with electron capture detection (Nishikawa and Hayakawa 1986). Kissel etal. (1981) emphasize that water samples from potential acrolein treatment systems require the use of water from that system in preparing blanks, controls, and standards and that acrolein measurements should be made at the anticipated use concentrations. 

High-performance liquid chromatography (HPLC) grade Solvents of suitable purity for use in liquid chromatography procedures. 

G. L. Lensmeyer and M. A. Evenson, Stabilized analysis of antidepressant drugs by solvent-recycled liquid chromatography procedure and proposed resolution mechanisms of chromatography, Clin. Chem., 50 1774 (1984). 

G. Carignan, B. A., B. A. Lodge, and W. Skakum, General re-versed-phase high-performance liquid chromatography procedure for the analysis of oral contraceptive formulations, J. Chromatogr., 375 470(1984). 

Various workers [23-32] have studied the application of high performance liquid chromatography to the determination of PAHs in water samples. Hagenmaier et al. [31] used a reversed phase high-pressure liquid chromatography procedure for the determination of trace amounts of polycyclic aromatic hydrocarbons in water. Different column packing materials were tested, in conjunction with non polar stationary phases of various polarities, for separation efficiency, detection limits and long-term stability. The method was suitable for concentrations as low as 2ng L 1 in a IL sample. Compounds... 

Barua, A.B. 2001. Improved normal-phase and reversed-phase gradient high-performance liquid chromatography procedures for the analysis of retinoids and carotenoids in human serum, plant and animal tissues. J. Chromatogr. A 936 71-82. 

Part of this material (340 mg) was purified even more by reversed-phase medium-pressure liquid chromatography procedure using a column (2.5 x 45 cm) of Whatman LRP-I (C-18-bonded silica gel, 13-24 xm) eluted with a gradient from 15-60% acetonitrile in 0.1% TFA. Aliquots from the main peak were pooled and lyophilized to give 75 mg of almost pure peptide, which counted a 18.5% yield based on resin incorporation. The final purification was achieved by a preparative RP-HPLC... 

Recently, minute doses of nonradioactive filtration markers such as iothalamate and diatrizoate meglumine (or Hypaque) have been used for assessing GFR. The development of high-performance liquid chromatography procedures for measurement of these nonradioactive filtration markers in plasma and urine provides a practical alternative to the laboratorian wary of the hazards associated with a radiolabel. 

JR Deschamps, EK Hidreth, D Derr, JT August. A high-performance liquid chromatography procedure for the purification of mouse monoclonal antibodies. Anal Biochem 147 451, 1985. 

Preparative liquid chromatography procedures are described for isolating highly pure... 

Pawlosky, R., Flanagan, V., and Doherty, R. 2003. A mass spectrometric validated high-performance liquid chromatography procedure for the determination of folates in foods. J. Agric. Food Chem. 51 3726-3730. 

MA Kutnink, H Vannucchi, HE Sauberlich. A simple high performance liquid chromatography procedure for the determination of lV -methylnicotinamide in urine. J Liq Chromatogr 7 969-977, 1984. 

Table 1. Pyrolysis-Gas Liquid Chromatography Procedures and Conditions. 

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