High-performance liquid chromatography procedures, analysis derivatized

A large number of analytical methods for detection of CWA degradation products are available. Gas chromatography/mass spectrometry (GC/MS) and high performance liquid chromatography (HPLC) with fluorescence detection are the most widely used.6,7 However, limitations of these methods include complicated derivatization procedures, long analysis time, and difficulty in miniaturization. 

Recently we completed an evaluation of the effectiveness of the Aflatest (Vicam) immunoaffinity column for analysis of corn, peanuts and peanut butter for aflatoxin (11). In this procedure the sample is extracted with Me0H/H20, filtered, and the extract diluted to <30% MeOH with water. An aliquot is then applied to the immunoaffinity column. The column is then washed with water and the aflatoxins eluted with methanol. Total aflatoxins are then determined by solution fluorometry with bromine (SFB), or individual toxins by reverse phase high performance liquid chromatography with post column I2 derivatization (PCD). In the collaborative study corn samples naturally contaminated with aflatoxins and samples of corn, peanuts and peanut butter spiked at 30, 20 and 10 ng/g, were analyzed by 24 collaborators. The results of this study are tabulated in Table V. 

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