High-performance liquid chromatography phase

Cass, Q. B., Bassi, A. L., and Matlin, S. A. (1999). First direct resolution of gossypol enantiomers on a chiral high-performance liquid chromatography phase. Chirality 11, 46-49. 

High Performance Liquid Chromatography. Although chiral mobile phase additives have been used in high performance Hquid chromatography (hplc), the large amounts of solvent, thus chiral mobile phase additive, required to pre-equiUbrate the stationary phase renders this approach much less attractive than for dc and is not discussed here. 

DETERMINATION OF SODIUM ACYLISETHIONATE IN THE COMBI SOAP BY REVERSED - PHASE HIGH - PERFORMANCE LIQUID CHROMATOGRAPHY... 

As a method of research, has been used high-performance liquid chromatography in reversed - phase regime (RP HPLC). The advantage of the present method is the following the additional information about AIST and FAS composition (homologous distribution) simple preparation of samples (dilution of a CS sample of in a mobile phase). 

Selectivity of chromatographic separation is known to be varied by changing both the nonstationary phase composition and adsorbent nature. It is shown that the less are the values of the reached selectivity coefficient the higher are the requirements to column effectiveness. In this connection the choice of stationai y phase with high and predicted selectivity coefficient for the compounds being separated is still remains a topical problem of high-performance liquid chromatography. 

The aim of the work is investigate possibilities of application of Cartridges Packed DIAPAK for concentrating antibiotics Cefazoline and Levomycetine and analyze them by Reversed Phase High Performance Liquid Chromatography (RP HPLC). 

Commercial grades of PVP, K-15, K-30, K-90, and K-120 and the quaternized copolymer of vinylpyrrolidone and dimthylaminoethylmethacrylate (poly-VP/ DMAEMA) made by International Specialty Products (ISP) were used in this study. PEO standard calibration kits were purchased from Polymer Laboratories Ltd. (PL), American Polymer Standards Corporation (APSC), Polymer Standards Service (PSS), and Tosoh Corporation (TSK). In addition, two narrow NIST standards, 1923 and 1924, were used to evaluate commercial PEO standards. Deionized, filtered water, and high-performance liquid chromatography grade methanol purchased from Aldrich or Fischer Scientific were used in this study. Lithium nitrate (LiN03) from Aldrich was the salt added to the mobile phases to control for polyelectrolyte effects. 

PACKED CAPILLARY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-CAPILLARY ZONE ELECTROPHORESIS... 

W. G. Button, K. D. Nugent, T. K. Slattery, B. R. Summers and L. R. Snyder, Separation of proteins by reversed-phase high-performance liquid chromatography , 7. Chromatogr. 443 363-379(1988). 

K.-M. Chu, S.-M. Sliieh, S.-H. Wu and O. Y.-P. Hu, Enantiomeric separation of a cardiotonic agent pimobendan and its major active metabolite, UD-CG 212 BS, by coupled achiral-cliiral normal-phase high-performance liquid chromatography , 7. Chromatogr. Sci 30 171-176(1992). 

K. S. Boos and C. H. Grimm, High performance liquid chromatography integated with solid-phase exti action in bioanalysis using resti icted access precolumn packings . Trends Anal. Chem. 18 175-180(1999). 

Despite the difficulties caused by the rapidly expanding literature, the use of chiral stationary phases (CSPs) as the method of choice for analysis or preparation of enantiomers is today well established and has become almost routine. It results from the development of chiral chromatographic methods that more than 1000 chiral stationary phases exemplified by several thousands of enantiomer separations have been described for high-performance liquid chromatography (HPLC). 

High-performance liquid chromatography is in some respects more versatile than gas chromatography since (a) it is not limited to volatile and thermally stable samples, and (b) the choice of mobile and stationary phases is wider. 

Commercially available poiybrominated aromatic ethers have been analyzed by reversed phase high performance liquid chromatography. NMR spectra of material isolated by preparative methods served to identify the observed peaks as congeners of tetrabromo to nonabromo diphenyl ether. A bromination pathway was clearly indicated. 

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