Liquid chromatography-mass characterization

Figure 5.6 Positive-ion electrospray spectrum obtained from the major component in the LC-MS analysis of a purified recombinant 62 kDa protein using a Cig microbore 50 X 1 mm column and a flow rate of 50 p.lmin . The starting buffer (buffer A ) was 0.1% TEA in water, while the gradient buffer (buffer B ) consisted of 0.1% TEA in acetonitrile-water (9 1 vol/vol). The running conditions consisted of 0% B for 5 min, followed by a linear gradient of 100% B for 55 min. Reprinted from J. Chromatogr., B, 685, McAtee, C. P., Zhang, Y., Yarbough, P. O., Fuerst, T. R., Stone, K. L., Samander, S. and Williams, K. R., Purification and characterization of a recombinant hepatitis E protein vaccine candidate by liquid chromatography-mass spectrometry , 91-104, Copyright (1996), with permission from Elsevier Science.

Hiserodt, R. et al.. Characterization of powdered turmeric by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry/ Chromatogr. A, 740, 51, 1996.,... [Pg.85]

Wang, H., Race, E.J., and Shrikhande, A.J., Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry, J. Agric. Food Chem.,... [Pg.271]

Maynard, D.M., Masuda, J., Yang, X., Kowalak, J.A., Markey, S.P. (2004). Characterizing complex peptide mixtures using a multi-dimensional liquid chromatography-mass spectrometry system Saccharomyces cerevisiae as a model system. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 810, 69-76. [Pg.286]

Opiteck, G.J., Jorgenson, J.W., MacNair, J.E., Moseley, M.A., 3rd (1997). Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides. Rapid separation and characterization of protein and peptide mixtures using 1.5 microns diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry. Anal. Chem. 69, 2283-2291. [Pg.287]

Sanz-Nebot, V., Benavente, F., Barbosa, J. (2002). Liquid chromatography-mass spectrometry and capillary electrophoresis combined approach for separation and characterization of multicomponent peptide mixtures application to crude products of leuprolide synthesis. J. Chromatogr. A 950, 99-111. [Pg.383]

Cheguillaume, G., Buchmann, W., Desmazieres, B., Tortajada, J. (2004). Liquid chromatography-mass spectrometry hyphenation for exhaustive and unambiguous characterization of polyoxyethylene surfactants. Chromatographia 60(9/10), 561-566. [Pg.443]

W. Potts, R. van Horn, K. Anderson, T. Blake, E. Garver, G. Joseph, G. Dreyer, A. Shu, R. Heys, K. L. Fong, Characterization of the Metabolites of the Peptidomimetic Human Immunodeficiency Virus Type 1 Protease Inhibitor SK F 107461 in Rats Using Liquid Chromatography/Mass Spectrometry , Drug Metab. Dispos. 1995, 23, 799 - 805. [Pg.381]

Mitamura K, Yatera M, Shimada K. 2000. Studies on neurosteroids. Part XIII. Characterization of catechol estrogens in rat brains using liquid chromatography-mass spectrometry-mass spectrometry. Analyst 125 811. [Pg.173]

Semak I, Korik E, Naumova M, Wortsman J, Slominski A. 2004. Serotonin metabolism in rat skin characterization by liquid chromatography-mass spectrometry. Arch Biochem Biophys 421 61. [Pg.175]

Shimada K, Mukai Y, Yago KJ. 1998. Studies of neurosteroids. VII. Characterization of pregnenolone, its sulfate and dehydroepiandrosterone in rat brains using liquid chromatography/mass spectrometry. J Liq Chrom Rel Technol 21 765-775. [Pg.191]

As a result of advances in detection technology, newer HPLC detection techniques may be utilized. For example, evaporative light-scattering detection and refractive index detection may be used to quantify impurities with poor or no UV chromophore. Conductivity detection may also be used. In some cases, when no chromophore is present, chemical derivatization may be used to add a chromophore. Increasingly, tandem techniques such as liquid chromatography mass spectrometry (LC-MS) and GC-MS are utilized for impurity characterization. The highly selective nature of LC-MS ensures that few impurities are undetected. [Pg.12]

Slimestad, R. and Hostettmann, K., Characterization of phenolic constituents from juvenile and mature needles of Norway spruce by means of high performance liquid chromatography-mass spectrometry, Phytochem. Anal, 7, 42, 1996. [Pg.128]

Maillard, M.-N., Giampaoli, P., and Cuvelier, M.-E., Atmospheric pressure chemical ionization (APCI) liquid chromatography-mass spectrometry characterization of natural antioxidants, Talanta, 43, 339, 1996. [Pg.134]

Grayer, R.J. et al.. The application of atmospheric pressure chemical ionization liquid chromatography-mass spectrometry in the chemotaxonomic study of flavonoids characterization of flavonoids from Ocimum gratissimum var., Phytochem. Anal., 11, 257, 2000. [Pg.907]

This extension of the Smith analysis, involving methylation of the polyalcohol, has been studied in detail by Bose, both on model systems and on the Ti fructan.84 When a ffuctan is used, this method may give l-hydroxy-3-methoxy-2-propanone and l,3-dimethoxy-2-propa-none, together with methylated glycerols. These compounds may react further under methanolysis conditions to yield 2,5-dimethoxy-2,5-bis(methoxymethyl)-l,4-dioxane and l,3-dimethoxy-2-propanone dimethyl acetal, respectively. Several of these compounds may be obtained from sucrose by a model reaction. In view of the wide variety of hydroxy compounds obtained in this study,84 their separation and identification as benzeneboronates was investigated, and certain of the latter derivatives were purified by gas-liquid chromatography. The characterization of 1,2- and 1,3-diols by the mass spectra of their cyclic benzeneboronates has also been described.634... [Pg.98]

Increased use of liquid chromatography/mass spectrometry (lc/ms) for structural identification and trace analysis has become apparent. Thermo-spray lc/ms has been used to identify by-products in phenyl isocyanate precolumn derivatization reactions Liquid chromatography/thermospray mass spectrometric characterization of chemical adducts of DNA formed during in vitro reaction lias been proposed as an analytical technique to detect and identify those contaminants in aqueous environmental samples which have a propensity to be genotoxic, t.e.. to covalently bond to DNA. [Pg.1627]

D Barcelo, J Albaiges. Characterization of organophosphorus compounds and phenylurea herbicides by positive and negative ion thermospray liquid chromatography-mass spectrometry. J Chromatogr 474 163-173,1989. [Pg.760]

When this method, in a slightly modified form, was applied to Salmonella free lipid A, mainly two compounds were liberated which were detected by gas-liquid chromatography and characterized by mass spectrometry. The spectrum of the first compound was identical with that of authentic 3-hydroxytetradecanoic acid, 3-0-acylated by dodecanoic acid (3-0-dodecanoyl-tetradecanoic acid methyl ester, 3-0(12 0)-14 0) (27). The mass spectrum of the second peak showed, inter alia, characteristic fragments at 496 (M ), 465 (M-31), 239, 240 and 241, thus corresponding to 3-0-hexadecanoyl-tetradecanoic acid methyl ester (3—0(16 0)—14 0). Therefore, the two components present in Salmonella lipid A in amide linkage and released by the above described procedure... [Pg.205]

B. Barroso, R. Dijkstra, M. Geerts, F. Lagerwerf, P. van Veelen, and A. de Ru, On-line high-performance liquid chromatography/mass spectrometric characterization of native oligosaccharides from glycoproteins, Rapid Common. Mass Spectrom., 16 (2002) 1320-1329. [Pg.133]

Tiller, P. R. Land, A. P Jardine, I. Murphy, D. M. Sozio, R. Ayrton, A. Schaefer, W. H. 1998. Application of liquid chromatography-mass spectrometry analyses to the characterization of novel glyburide metabolites formed in vitro. /. Chromatogr. A, 794,15-25. [Pg.229]

Zeng, L. Burton, L. Yung, K. Shushan, B. Kassel, D. B. 1998b. Automated analytical/preparative high-performance liquid chromatography-mass spectrometry system for the rapid characterization and purification of compound libraries. /. Chromatogr. A, 794,3-13. [Pg.234]

Guo X, Bruins AP, Covey TR (2006) Characterization of typical chemical background interferences in atmospheric pressure ionization liquid chromatography-mass spectrometry. Rapid Commun Mass Spectrom 20 3145-3150... [Pg.124]

We will briefly highlight some examples using NMR methods [14, 19] but the remainder of this chapter will focus on MS and liquid chromatography-mass spectrometry (LC-MS) metabolomics [5, 15, 18]. We should also point out that there are a number of examples of both NMR and MS methods being used in the discovery of medicinally important biomarkers [23, 31] however, this review will focus more on the use of metabolomics to characterize proteins. [Pg.140]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...