High-performance liquid chromatography on-line

Hansen, S.H. et al.. High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L., Anal. Chem., 71, 5235, 1999. 

Apffel, A. Chakel, J. Udiavar, S. Hancock, W. S. Souders, C. Pungor, E. Jr. 1995. Application of capillary electrophoresis, high-performance liquid chromatography, on-line electrospray mass spectrometry and matrix-assisted laser desorption ionization—time of flight mass spectrometry to the characterization of single-chain plasminogen activator. . /. Chromatogr. A, 717,41-60. 

L. N. Liang, G. B. Jiang, J. G. Liu, J. T. Hu, Speciation analysis of mercury in seafood by using high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry via a post column microwave digestion, Anal. Chim. Acta, Ml (2003), 131-137. 

Wangkam, S., Pergantis, S. A. High-speed separation of arsenic compounds using narrow-bore high-performance liquid chromatography on-line with inductively coupled plasma mass spectrometry. J Anal At Spectrom 2000, 15, 627-633. 

Wei, M.-C., et al., Determination of organic acids in fermentation products of milk with high performance liquid chromatography/on-lined micro-dialysis, Chwmatographia, 54, 601, 2001. 

T. Storm, C. Hartig, T. Reemtsma and M. Jekel, Exact mass measurements on-line with high-performance liquid chromatography on a quadrupole mass spectrometer. Anal. Chem., 73 (2001) 589-595. 

Figure 5.3 Analysis of 100 ml of (a) surface water and (b) drinking water sample spiked with 0.1 pig/ml of microcystins, using column-switching HPLC 1, microcystin-RR 2, microcystin-YR 3, microcystin-LR. Reprinted from Journal of Chromatography A, 848, H. S. Lee et al, On-line trace enrichment for the simultaneous determination of microcystins in aqueous samples using high performance liquid chromatography with diode-array detection , pp 179-184, copyright 1999, with permission from Elsevier Science.

Y. Oda, N. Asakawa, Y. Yoshida and T. Sato, On-line determination and resolution of the enantiomers of ketoprofen in plasma using coupled achiral-cliiral high-performance liquid chromatography , 7. Pharm. Biomed. Anal. 10 81-87 (1992). 

For off-bead analysis, coupling between chromatographic separation and mass spectrometric detection has proven especially powerful. The combination between high performance liquid chromatography (HPLC) and electrospray ionisation mass spectrometry has the advantage that purity of product mixtures can be coupled on-line with the product identification. 

Hentschel, P. et ah. Structure elucidation of deoxylutein 11 isomers by on-line capillary high performance liquid chromatography- H nuclear magnetic resonance spectroscopy, J. Chromatogr. A, 1112, 285, 2006. 

Li, H. and Westerholm, R., Determination of mono- and di-nitro polycyclic aromatic hydrocarbons by on-line reduction and high-performance liquid chromatography with chemiluminescence detection, /. Chromatogr. A, 664,177, 1994. 

Suzuki-Sawada, J., Umeda, Y., Kondo, A., and Kato, I., Analysis of oligosaccharides by on-line high-performance liquid chromatography and ion-spray spectrometry, Anal. Biochem., 207, 203, 1992. 

Stuting, H. H., Krull, I. S., Mhatre, R., Krzysko, S. C., and Barth, H. G., High performance liquid chromatography of biopolymers using on-line laser lightscattering technique, LC-GC, 7, 402, 1989. 

Because carotenoids are light- and oxygen-sensitive, a closed-loop hyphenated technique such as the on-line coupling of high performance liquid chromatography (HPLC) together with nuclear magnetic resonance (NMR) spectroscopy can be used for the artifact-free structural determination of the different isomers. 

J. Chao, J. Liu, M. Wen, J. Liu, G. Cai and G. Jiang, Determination of sulfonylurea hericides by continuous-flow hquid membrane extraction on-line coupled with high-performance liquid chromatography. J. ChromatogrA 955 (2002) 183-189. 

A. Rehorek, K. Urbig, R. Meurer, C. Schafer, A. Plum and G. Braun, Monitoring of azo dye degradation processes in a bioreactor by on-line high-performance liquid chromatography. J. Chromatogr.A, 949 (2002) 263-268. 

Olesen OV, Plougmann P, Linnet K. 2000. Determination of nortriptyline in human serum by fully automated solid-phase extraction and on-line high-performance liquid chromatography in the presence of antipsychotic drugs. J Chromatogr B 746(2) 233-239. 

Weigmann H, Hartter S, Hierake C. 1998. Automated determination of clomipramine and its major metabolites in human and rat serum by high-performance liquid chromatography with on-line column-switching. J Chromatogr B Biomed Sci App 710(1-2) 227-233. 

Weigmann H, Hartter S, Maehrlein S, Kiefer W, Kramer G, et al. 2001. Simultaneous determination of olanzapine, clozapine and demethylated metabolites in serum by on-line column-switching high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 759(1) 63-71. 

Peters R, Hellenbrand J, Mengerink Y, Wal Van der S. 2004. On-line determination of carboxylic acids, aldehydes and ketones by high-performance liquid chromatography-diode array detection-atmospheric pressure chemical ionization mass spectrometry after derivatization with 2-nitrophenylhydrazine. J Chromatogr A 1031 35. 

Hua, Y., et al., On-line high-performance liquid chromatography-electrospray ionization mass spectrometry for the determination of brevetoxins in red tide algae, Anal. Chem., 67, 11, 1815, 1995. 

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