Mass spectrometric characterization

In order to overcome these difficulties, we developed a precision synthesis method on the basis of high-resolution size separation and mass spectrometric characterization. 

Figure 2. Experimental flow chart showing fractionation of as-prepared Au SR clusters by size and mass spectrometric characterization.

McClean S, O Kane EJ, Smyth WR 2000. Electrospray ionization-mass spectrometric characterization of selected antipsychotic drugs and their detection and determination in human hair samples by liquid chromatography-tandem mass spectrometry. J Chromatogr B Biomed Sci Appl 740 141. 

Wei, L.Y. Gas chromatographic and mass spectrometric characterization of terbufos sulfoxide and its hydration product, J. Environ. Sci Health, B25(5) 607-613, 1990. 

Thomas, C.E., Kelleher, N.L. and Mizzen, C.A. (2006) Mass spectrometric characterization of human histone H3 ... 

Novotny M, Merli F, Wiesler D, et al. 1982. Fractionation and capillary gas chromatographic-mass spectrometric characterization of the neutral components in marijuana and tobacco smoke condensates. J Chromatogr 238(1) 141-150. 

Gas Chromatography-Mass Spectrometric Characterization of Polynuclear Aromatic Hydrocarbons in Particulate Diesel Emissions... 

Increased use of liquid chromatography/mass spectrometry (lc/ms) for structural identification and trace analysis has become apparent. Thermo-spray lc/ms has been used to identify by-products in phenyl isocyanate precolumn derivatization reactions Liquid chromatography/thermospray mass spectrometric characterization of chemical adducts of DNA formed during in vitro reaction lias been proposed as an analytical technique to detect and identify those contaminants in aqueous environmental samples which have a propensity to be genotoxic, t.e.. to covalently bond to DNA. 

The relatively high volatility of complexes of this type makes their purification by sublimation routine and also lends itself to mass spectrometric characterization. To date a complex of stoichiometry M[N(SiMe3)2]4 has not been isolated, even the large metals Th4+ and U4+ giving only partially substituted derivatives, e.g. QTh[N(SiMe3)2]3.98... 

Vetter W, Alder L, Palavinskas R (1999) Mass Spectrometric Characterization of Ql, a C9H3CI7N2 Contaminant in Environmental Samples. Rapid Commun Mass Spectrom 13 2118... 

Mass spectrometric characterization of highly hydrogenated fullerenes showed also that some intermediate products are formed before the cage structure of fulleranes collapses fragmented fulleranes. The name fragmented fullerenes is used when fullerene molecule loses some carbon atoms, thus forming e.g. C58, C56, C54 etc. These molecules can be produced from C60 in the form of atomic clusters in gas phase and were typically observed only by mass spectrometry (Murry et al. 1993 Hathiramani et al. 2000 Dunser et al. 1997). Fragmented fulleranes are stable and could be obtained in bulk amounts, see next section for more details. 

Mass Spectrometric Characterization of Complex Fullerane Mixtures... 

B. Barroso, R. Dijkstra, M. Geerts, F. Lagerwerf, P. van Veelen, and A. de Ru, On-line high-performance liquid chromatography/mass spectrometric characterization of native oligosaccharides from glycoproteins, Rapid Common. Mass Spectrom., 16 (2002) 1320-1329. 

C., Gruen, D. M. Stock, L. M. 1991 High yield synthesis, extraction and mass spectrometric characterization of fullerenes C60 to C266. J. Am. chem. Soc. 113, 7499-7503. 

Pi N, Meyers CLF, Pacholec M et al (2004) Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton. Proc Natl Acad Sci USA 101 10036-10041... 

E Mortz, T Sareneva, S Haebel, I Julkunen, P RoepstrofF. Mass spectrometric characterization of glycosylated interferon-y variants separated by gel electrophoresis. Electrophoresis 17 925-931, 1996. 

Mazzarino M et al (2010) Mass spectrometric characterization of tamoxifene metabolites in human urine utilizing different scan parameters on liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 24 749-760... 

El Hamidi, A., Tirsoaga, A., Novikov, A., Hussein, A., Caroff, M. Microextraction of bacterial lipid A easy and rapid method for mass spectrometric characterization. J Lipid Res 46 (2005) 1773-1778. 

Cheng, X.H., Morin, P.E., Harms, A.C. et al. (1996) Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.l DNA binding domain. Anal. Biochem., 239 (1), 35 40. 

Both cationic and anionic precursors were used to generate the 02SOH radical (44) which is the presumed intermediate of OH radical attack on S02 to form S03 in the last step of the sulfur oxidation cascade [198]. The hydrogen sulfite cation was produced by dissociative ionization of methanesulfonic acid and used to generate radical 44, albeit in a low yield [209]. A improved preparation and NR mass spectrometric characterization of 44 relied on an anion precursor, 02SOH , which was made by termolecular associative reaction of S02 with OH-[210]. Neutralization with Xe of 02SOH- produced stable radical 44 that gave rise to a dominant survivor cation in the -NR+ mass spectrum [211]. 

In the field of natural red algal galactans, MS was used for the first time in the structural analysis of odonthalan.353 Galactose and 6-O-methylgalactose, the hydrolysis products of the polysaccharide, were separated by column chromatography and identified by MS in the form of their di-O-isopropylidene derivatives. Cyclic derivatives of this type had been shown previously to be especially suitable for mass-spectrometric characterization of the structure and (in certain instances) stereochemistry of monosaccharides.349,354 A combination of GLC and MS was also used for identification of methyl 4.6-O-0 -methoxycarbonylethylidene)-D-galactosides (as their trifluoroacetates) in the methanolysis products of Ji-carrageenan from Petrocelis middendorfii,355... 

The mass spectrometric behavior of the isomeric 2-oxo-2H- and 4-oxo-4 -6,7,8,9-tetrahydropyrido[l,2-<3]pyrimidines was studied under electron-impact induced polarization (97RCM664). The molecular ion of the 2-OXO-2H isomer appeared to be much more stable than that of the 4-oxo-4 isomer. The fragmentation of the molecular ion (M+) of the 4-oxo-4 isomer is related mostly to the saturated piperidine ring, whereas that of the 2-oxo-2H isomer is much more selective, the only significant process is the primary loss of a CO molecule from the pyrimidinone ring via contraction of the ring. Electrospray ionization quadrupole ion-trap mass spectrometric characterization of risperidon (11) was presented and a possible mechanism for the observed fragmentation pattern was... 

S. Wang, Y. H. Liu, C. K. Smith, and B. N. Pramanik, Mass spectrometric characterization of MEKl phosphorylation during expression in insect cells, presented at the 52nd ASMS Conference on Mass Spectrometry, May 23-27,2004, Nashville, Tennessee. 

Tomy GT (1997) The mass spectrometric characterization of polychlorinated n-alkanes and the methodology for their analysis in the environment. PhD Thesis, University of Manitoba... 

Faix O, Meier D, Fortmann 1 (1990a) Thermal degradation products of wood. Gas chromatographic separation and mass spectrometric characterization of monomeric lignin derived products. Holz Roh- Werkst 48 281-285... 

Canton L. and Grimalt J. O. (1992) Gas chromatographic-mass spectrometric characterization of polycyclic aromatic hydrocarbon mixtures in polluted coastal sediments. J. Chromatogr. 607, 279-286. 

An obvious next step is the combination of the two techniques, BIA/MS with bioreactive mass spectrometry probe tips. Such an approach would thereby allow (all on a single surface), the real-time observance of affinity interaction followed by enzymatic modification and mass spectrometric characterization of retained ligands. 

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